Probing the Viromic Frontiers

Probing the Viromic Frontiers

ABSTRACT Modern molecular technology, and particularly high-throughput sequencing (HTS), has revolutionized virus discovery and expanded the depth and breadth of the virome. Recent HTS was used to identify and discover a previously undescribed member of the family Flaviviridae that has genomic featu...

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Autor principal: Angela L. Rasmussen
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2015
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Microbiology
QR1-502
Acceso en línea:https://doaj.org/article/577aae8d6ebd4e01a0e2415634516892
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spelling oai:doaj.org-article:577aae8d6ebd4e01a0e24156345168922021-11-15T15:41:24ZProbing the Viromic Frontiers10.1128/mBio.01767-152150-7511https://doaj.org/article/577aae8d6ebd4e01a0e24156345168922015-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01767-15https://doaj.org/toc/2150-7511ABSTRACT Modern molecular technology, and particularly high-throughput sequencing (HTS), has revolutionized virus discovery and expanded the depth and breadth of the virome. Recent HTS was used to identify and discover a previously undescribed member of the family Flaviviridae that has genomic features characteristic of both hepaciviruses and pegiviruses. This virus, designated human hepegivirus-1 (HHpgV-1), may represent a previously undescribed new genus in the Flaviviridae family with implications for public health and blood supply safety. Detecting uncharacterized viruses such as HHpgV-1 in clinical samples requires an unbiased screening method that is as sensitive as PCR, while simultaneously detecting multiple rare viral sequences. The virome-capture-sequencing platform for vertebrate viruses (VirCapSeq-VERT) uses positive-selection oligonucleotide capture to sensitively detect sequences from every known vertebrate virus, even in high-background specimens with low-abundance viruses. VirCapSeq-VERT can also detect uncharacterized viruses with sequence homology to known viruses, enabling a new paradigm for virus detection.Angela L. RasmussenAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 6 (2015)
institution DOAJ
collection DOAJ
language EN
topic Microbiology
QR1-502
spellingShingle Microbiology
QR1-502
Angela L. Rasmussen
Probing the Viromic Frontiers
description ABSTRACT Modern molecular technology, and particularly high-throughput sequencing (HTS), has revolutionized virus discovery and expanded the depth and breadth of the virome. Recent HTS was used to identify and discover a previously undescribed member of the family Flaviviridae that has genomic features characteristic of both hepaciviruses and pegiviruses. This virus, designated human hepegivirus-1 (HHpgV-1), may represent a previously undescribed new genus in the Flaviviridae family with implications for public health and blood supply safety. Detecting uncharacterized viruses such as HHpgV-1 in clinical samples requires an unbiased screening method that is as sensitive as PCR, while simultaneously detecting multiple rare viral sequences. The virome-capture-sequencing platform for vertebrate viruses (VirCapSeq-VERT) uses positive-selection oligonucleotide capture to sensitively detect sequences from every known vertebrate virus, even in high-background specimens with low-abundance viruses. VirCapSeq-VERT can also detect uncharacterized viruses with sequence homology to known viruses, enabling a new paradigm for virus detection.
format article
author Angela L. Rasmussen
author_facet Angela L. Rasmussen
author_sort Angela L. Rasmussen
title Probing the Viromic Frontiers
title_short Probing the Viromic Frontiers
title_full Probing the Viromic Frontiers
title_fullStr Probing the Viromic Frontiers
title_full_unstemmed Probing the Viromic Frontiers
title_sort probing the viromic frontiers
publisher American Society for Microbiology
publishDate 2015
url https://doaj.org/article/577aae8d6ebd4e01a0e2415634516892
work_keys_str_mv AT angelalrasmussen probingtheviromicfrontiers
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