Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients
Micro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the miRNA profile of...
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2021
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oai:doaj.org-article:577ff739dce0467d943d6fe40ec95f682021-11-25T16:49:45ZDifferent HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients10.3390/biomedicines91116272227-9059https://doaj.org/article/577ff739dce0467d943d6fe40ec95f682021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1627https://doaj.org/toc/2227-9059Micro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the miRNA profile of PBMCs for each type of infection. We massively sequenced small RNAs of PBMCs from 117 HIV+ infected patients: 45 HIV+ patients chronically infected with HCV (HIV/HCV+), 36 HIV+ that spontaneously clarified HCV after acute infection (HIV/HCV-) and 36 HIV+ patients without previous HCV infection (HIV). Thirty-two healthy patients were used as healthy controls (HC). Differential expression analysis showed significantly differentially expressed (SDE) miRNAs in HIV/HCV+ (<i>n</i> = 153), HIV/HCV- (<i>n</i> = 169) and HIV (<i>n</i> = 153) patients. We found putative dysregulated pathways, such as infectious-related and PI3K signaling pathways, common in all contrasts. Specifically, putatively targeted genes involved in antifolate resistance (HIV/HV+), cancer-related pathways (HIV/HCV-) and HIF-signaling (HIV) were identified, among others. Our findings revealed that HCV strongly influences the expression profile of PBMCs from HIV patients through the disruption of its miRNome. Thus, different HCV exposure can be identified by specific miRNA signatures in PBMCs.Daniel Valle-MillaresÓscar Brochado-KithLuz Martín-CarboneroLourdes Domínguez-DomínguezPablo RyanIgnacio De los SantosSara De la FuenteJuan M. CastroMaría LagardeGuillermo CuevasMario Mayoral-MuñozMariano MatarranzVictorino DíezAlicia Gómez-SanzPaula Martínez-RománCelia Crespo-BermejoClaudia PalladinoMaría Muñoz-MuñozMaría A. Jiménez-SousaSalvador ResinoVerónica BrizAmanda Fernández-Rodríguezon Behalf of Multidisciplinary Group of Viral Coinfection HIV/Hepatitis (COVIHEP)MDPI AGarticleHCVHIVmicroRNAsspontaneous viral clearancehigh throughput sequenceBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1627, p 1627 (2021) |
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| topic |
HCV HIV microRNAs spontaneous viral clearance high throughput sequence Biology (General) QH301-705.5 |
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HCV HIV microRNAs spontaneous viral clearance high throughput sequence Biology (General) QH301-705.5 Daniel Valle-Millares Óscar Brochado-Kith Luz Martín-Carbonero Lourdes Domínguez-Domínguez Pablo Ryan Ignacio De los Santos Sara De la Fuente Juan M. Castro María Lagarde Guillermo Cuevas Mario Mayoral-Muñoz Mariano Matarranz Victorino Díez Alicia Gómez-Sanz Paula Martínez-Román Celia Crespo-Bermejo Claudia Palladino María Muñoz-Muñoz María A. Jiménez-Sousa Salvador Resino Verónica Briz Amanda Fernández-Rodríguez on Behalf of Multidisciplinary Group of Viral Coinfection HIV/Hepatitis (COVIHEP) Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
| description |
Micro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the miRNA profile of PBMCs for each type of infection. We massively sequenced small RNAs of PBMCs from 117 HIV+ infected patients: 45 HIV+ patients chronically infected with HCV (HIV/HCV+), 36 HIV+ that spontaneously clarified HCV after acute infection (HIV/HCV-) and 36 HIV+ patients without previous HCV infection (HIV). Thirty-two healthy patients were used as healthy controls (HC). Differential expression analysis showed significantly differentially expressed (SDE) miRNAs in HIV/HCV+ (<i>n</i> = 153), HIV/HCV- (<i>n</i> = 169) and HIV (<i>n</i> = 153) patients. We found putative dysregulated pathways, such as infectious-related and PI3K signaling pathways, common in all contrasts. Specifically, putatively targeted genes involved in antifolate resistance (HIV/HV+), cancer-related pathways (HIV/HCV-) and HIF-signaling (HIV) were identified, among others. Our findings revealed that HCV strongly influences the expression profile of PBMCs from HIV patients through the disruption of its miRNome. Thus, different HCV exposure can be identified by specific miRNA signatures in PBMCs. |
| format |
article |
| author |
Daniel Valle-Millares Óscar Brochado-Kith Luz Martín-Carbonero Lourdes Domínguez-Domínguez Pablo Ryan Ignacio De los Santos Sara De la Fuente Juan M. Castro María Lagarde Guillermo Cuevas Mario Mayoral-Muñoz Mariano Matarranz Victorino Díez Alicia Gómez-Sanz Paula Martínez-Román Celia Crespo-Bermejo Claudia Palladino María Muñoz-Muñoz María A. Jiménez-Sousa Salvador Resino Verónica Briz Amanda Fernández-Rodríguez on Behalf of Multidisciplinary Group of Viral Coinfection HIV/Hepatitis (COVIHEP) |
| author_facet |
Daniel Valle-Millares Óscar Brochado-Kith Luz Martín-Carbonero Lourdes Domínguez-Domínguez Pablo Ryan Ignacio De los Santos Sara De la Fuente Juan M. Castro María Lagarde Guillermo Cuevas Mario Mayoral-Muñoz Mariano Matarranz Victorino Díez Alicia Gómez-Sanz Paula Martínez-Román Celia Crespo-Bermejo Claudia Palladino María Muñoz-Muñoz María A. Jiménez-Sousa Salvador Resino Verónica Briz Amanda Fernández-Rodríguez on Behalf of Multidisciplinary Group of Viral Coinfection HIV/Hepatitis (COVIHEP) |
| author_sort |
Daniel Valle-Millares |
| title |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
| title_short |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
| title_full |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
| title_fullStr |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
| title_full_unstemmed |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
| title_sort |
different hcv exposure drives specific mirna profile in pbmcs of hiv patients |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/577ff739dce0467d943d6fe40ec95f68 |
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