Electronic control of redox reactions inside Escherichia coli using a genetic module.

Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we...

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Autores principales:	Moshe Baruch, Sara Tejedor-Sanz, Lin Su, Caroline M Ajo-Franklin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57832763d784496d80451e9843f0dda8
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id	oai:doaj.org-article:57832763d784496d80451e9843f0dda8
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spelling	oai:doaj.org-article:57832763d784496d80451e9843f0dda82021-12-02T20:12:50ZElectronic control of redox reactions inside Escherichia coli using a genetic module.1932-620310.1371/journal.pone.0258380https://doaj.org/article/57832763d784496d80451e9843f0dda82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258380https://doaj.org/toc/1932-6203Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism.Moshe BaruchSara Tejedor-SanzLin SuCaroline M Ajo-FranklinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0258380 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moshe Baruch Sara Tejedor-Sanz Lin Su Caroline M Ajo-Franklin Electronic control of redox reactions inside Escherichia coli using a genetic module.
description	Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism.
format	article
author	Moshe Baruch Sara Tejedor-Sanz Lin Su Caroline M Ajo-Franklin
author_facet	Moshe Baruch Sara Tejedor-Sanz Lin Su Caroline M Ajo-Franklin
author_sort	Moshe Baruch
title	Electronic control of redox reactions inside Escherichia coli using a genetic module.
title_short	Electronic control of redox reactions inside Escherichia coli using a genetic module.
title_full	Electronic control of redox reactions inside Escherichia coli using a genetic module.
title_fullStr	Electronic control of redox reactions inside Escherichia coli using a genetic module.
title_full_unstemmed	Electronic control of redox reactions inside Escherichia coli using a genetic module.
title_sort	electronic control of redox reactions inside escherichia coli using a genetic module.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/57832763d784496d80451e9843f0dda8
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_version_	1718374823810301952

Electronic control of redox reactions inside Escherichia coli using a genetic module.

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