Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats

Mehwish Yousaf,1 Asima Tayyeb,2 Gibran Ali1 1National Centre of Excellence in Molecular Biology, University of the Punjab, 2School of Biological Sciences, University of the Punjab, Lahore, Pakistan Abstract: Culturing of primary hepatocytes and stem cell-derived hepatocytes faces a major issue of de...

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Autores principales:	Yousaf M, Tayyeb A, Ali G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Liver adhesion prenatal postnatal Cytology QH573-671
Acceso en línea:	https://doaj.org/article/578e0f7826784a5899541d6a44e4ea78
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spelling	oai:doaj.org-article:578e0f7826784a5899541d6a44e4ea782021-12-02T02:18:09ZExpression profiling of adhesion proteins during prenatal and postnatal liver development in rats1178-6957https://doaj.org/article/578e0f7826784a5899541d6a44e4ea782017-09-01T00:00:00Zhttps://www.dovepress.com/expression-profiling-of-adhesion-proteins-during-prenatal-and-postnata-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957Mehwish Yousaf,1 Asima Tayyeb,2 Gibran Ali1 1National Centre of Excellence in Molecular Biology, University of the Punjab, 2School of Biological Sciences, University of the Punjab, Lahore, Pakistan Abstract: Culturing of primary hepatocytes and stem cell-derived hepatocytes faces a major issue of dedifferentiation due to absence of cell–cell adhesion and 3D structures. One of the possible ways to eliminate the problem of dedifferentiation is mimicking the expression pattern of adhesion proteins during the normal developmental process of liver cells. The purpose of this study was to evaluate the expression pattern of some key adhesion proteins, namely, E-cadherin, N-cadherin, epithelial CAM (EpCAM), intracellular CAM (ICAM), collagen 1α1, α-actinin, β-catenin and vimentin, in the liver tissue during prenatal and postnatal stages. Furthermore, differences in their expression between prenatal, early postnatal and adult stages were highlighted. Wistar rats were used to isolate livers at prenatal Day 14 and 17 as well as on postnatal Day 1, 3, 7 and 14. The liver from adult rats was used as control. Both conventional and real-time quantitative polymerase chain reactions (PCRs) were performed. For most of the adhesion proteins such as E-cadherin, N-cadherin, EpCAM, ICAM, collagen 1α1 and α-actinin, low expression was observed around prenatal Day 14 and an increasing expression was observed in the postnatal period. Moreover, β-catenin and vimentin showed higher expression in the early prenatal period, which decreased gradually in the postnatal period, but still this low expression was considerably higher than that in the adult control rats. This basic knowledge of the regulation of expression of adhesion proteins during different developmental stages indicates their vital role in liver development. This pattern can be further studied and imitated under in vitro conditions to achieve better cell–cell interactions. Keywords: CAMS, cadherins, embryonic, hepaticYousaf MTayyeb AAli GDove Medical PressarticleLiveradhesionprenatalpostnatalCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 10, Pp 21-28 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Liver adhesion prenatal postnatal Cytology QH573-671
spellingShingle	Liver adhesion prenatal postnatal Cytology QH573-671 Yousaf M Tayyeb A Ali G Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats
description	Mehwish Yousaf,1 Asima Tayyeb,2 Gibran Ali1 1National Centre of Excellence in Molecular Biology, University of the Punjab, 2School of Biological Sciences, University of the Punjab, Lahore, Pakistan Abstract: Culturing of primary hepatocytes and stem cell-derived hepatocytes faces a major issue of dedifferentiation due to absence of cell–cell adhesion and 3D structures. One of the possible ways to eliminate the problem of dedifferentiation is mimicking the expression pattern of adhesion proteins during the normal developmental process of liver cells. The purpose of this study was to evaluate the expression pattern of some key adhesion proteins, namely, E-cadherin, N-cadherin, epithelial CAM (EpCAM), intracellular CAM (ICAM), collagen 1α1, α-actinin, β-catenin and vimentin, in the liver tissue during prenatal and postnatal stages. Furthermore, differences in their expression between prenatal, early postnatal and adult stages were highlighted. Wistar rats were used to isolate livers at prenatal Day 14 and 17 as well as on postnatal Day 1, 3, 7 and 14. The liver from adult rats was used as control. Both conventional and real-time quantitative polymerase chain reactions (PCRs) were performed. For most of the adhesion proteins such as E-cadherin, N-cadherin, EpCAM, ICAM, collagen 1α1 and α-actinin, low expression was observed around prenatal Day 14 and an increasing expression was observed in the postnatal period. Moreover, β-catenin and vimentin showed higher expression in the early prenatal period, which decreased gradually in the postnatal period, but still this low expression was considerably higher than that in the adult control rats. This basic knowledge of the regulation of expression of adhesion proteins during different developmental stages indicates their vital role in liver development. This pattern can be further studied and imitated under in vitro conditions to achieve better cell–cell interactions. Keywords: CAMS, cadherins, embryonic, hepatic
format	article
author	Yousaf M Tayyeb A Ali G
author_facet	Yousaf M Tayyeb A Ali G
author_sort	Yousaf M
title	Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats
title_short	Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats
title_full	Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats
title_fullStr	Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats
title_full_unstemmed	Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats
title_sort	expression profiling of adhesion proteins during prenatal and postnatal liver development in rats
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/578e0f7826784a5899541d6a44e4ea78
work_keys_str_mv	AT yousafm expressionprofilingofadhesionproteinsduringprenatalandpostnatalliverdevelopmentinrats AT tayyeba expressionprofilingofadhesionproteinsduringprenatalandpostnatalliverdevelopmentinrats AT alig expressionprofilingofadhesionproteinsduringprenatalandpostnatalliverdevelopmentinrats
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Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats

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