Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications

Yiming Zhu, 1 Zuochao Yao, 2 Yushu Liu, 3 Wen Zhang, 2 Lele Geng, 2 Tao Ni 2 1Department of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Huangpu, Shanghai, China; 2Department of Plastic and Reconstructive Surgery, Shanghai Ninth Pe...

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Autores principales:	Zhu Y, Yao Z, Liu Y, Zhang W, Geng L, Ni T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	substance p metal-organic framework nanoparticles hydrogel wound healing Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5791cedbbaa445a99742bc2c291643dc
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spelling	oai:doaj.org-article:5791cedbbaa445a99742bc2c291643dc2021-12-02T04:39:59ZIncorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications1178-2013https://doaj.org/article/5791cedbbaa445a99742bc2c291643dc2020-01-01T00:00:00Zhttps://www.dovepress.com/incorporation-of-ros-responsive-substance-p-loaded-zeolite-imidazolate-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yiming Zhu, 1 Zuochao Yao, 2 Yushu Liu, 3 Wen Zhang, 2 Lele Geng, 2 Tao Ni 2 1Department of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Huangpu, Shanghai, China; 2Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Huangpu, Shanghai, China; 3Department of Burns and Plastic Surgery, Binzhou Medical University Hospital, Binzhou City, Shandong Province, ChinaCorrespondence: Tao NiDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, No. 639 Zhizaoju Road, Huangpu District, Shanghai 200011, People’s Republic of ChinaEmail prsnt123456@126.comPurpose: Wound healing, especially of extensive full-thickness wounds, is one of the most difficult problems in clinical studies. In this study, we prepared a novel substance P (SP)-delivery system using zeolite imidazolate framework-8 (ZIF-8) nanoparticles.Methods: We synthesized ZIF-8 nanoparticles using a modified biomimetic mineralization method. We then coated SP-loaded ZIF-8 nanoparticles (SP@ZIF-8) with polyethylene glycol-thioketal (PEG-TK) to fabricate SP@ZIF-8-PEG-TK nanoparticles, and encapsulated them in injectable hydrogel composed of sodium alginate and pectin and cross-linked using calcium chloride. The final hydrogel wound dressing containing SP@ZIF-8-PEG-TK nanoparticles was called SP@ZIF-8-PEG-TK@CA.Results: The fabricated ZIF-8 nanoparticles had high SP-loading efficiency. SP-release assay showed that the SP@ZIF-8-PEG-TK nanoparticles maintained drug activity and showed responsive release under stimulation by reactive oxygen species. The SP@ZIF-8-PEG-TK nanoparticles promoted proliferation of human dermal fibroblasts, up-regulated expression levels of inflammation-related genes in macrophages, and exhibited favorable cytocompatibility in vitro. Full-thickness excision wound models in vivo confirmed that SP@ZIF-8-PEG-TK@CA dressings had excellent wound-healing efficacy by promoting an early inflammatory response and subsequent M2 macrophage polarization in the wound-healing process.Conclusion: In conclusion, these findings indicated that SP@ZIF-8-PEG-TK@CA dressings might be useful for wound dressing applications in the clinic.Keywords: substance P, metal-organic framework, nanoparticles, hydrogel, wound healingZhu YYao ZLiu YZhang WGeng LNi TDove Medical Pressarticlesubstance pmetal-organic frameworknanoparticleshydrogelwound healingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 333-346 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	substance p metal-organic framework nanoparticles hydrogel wound healing Medicine (General) R5-920
spellingShingle	substance p metal-organic framework nanoparticles hydrogel wound healing Medicine (General) R5-920 Zhu Y Yao Z Liu Y Zhang W Geng L Ni T Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications
description	Yiming Zhu, 1 Zuochao Yao, 2 Yushu Liu, 3 Wen Zhang, 2 Lele Geng, 2 Tao Ni 2 1Department of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Huangpu, Shanghai, China; 2Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Huangpu, Shanghai, China; 3Department of Burns and Plastic Surgery, Binzhou Medical University Hospital, Binzhou City, Shandong Province, ChinaCorrespondence: Tao NiDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, No. 639 Zhizaoju Road, Huangpu District, Shanghai 200011, People’s Republic of ChinaEmail prsnt123456@126.comPurpose: Wound healing, especially of extensive full-thickness wounds, is one of the most difficult problems in clinical studies. In this study, we prepared a novel substance P (SP)-delivery system using zeolite imidazolate framework-8 (ZIF-8) nanoparticles.Methods: We synthesized ZIF-8 nanoparticles using a modified biomimetic mineralization method. We then coated SP-loaded ZIF-8 nanoparticles (SP@ZIF-8) with polyethylene glycol-thioketal (PEG-TK) to fabricate SP@ZIF-8-PEG-TK nanoparticles, and encapsulated them in injectable hydrogel composed of sodium alginate and pectin and cross-linked using calcium chloride. The final hydrogel wound dressing containing SP@ZIF-8-PEG-TK nanoparticles was called SP@ZIF-8-PEG-TK@CA.Results: The fabricated ZIF-8 nanoparticles had high SP-loading efficiency. SP-release assay showed that the SP@ZIF-8-PEG-TK nanoparticles maintained drug activity and showed responsive release under stimulation by reactive oxygen species. The SP@ZIF-8-PEG-TK nanoparticles promoted proliferation of human dermal fibroblasts, up-regulated expression levels of inflammation-related genes in macrophages, and exhibited favorable cytocompatibility in vitro. Full-thickness excision wound models in vivo confirmed that SP@ZIF-8-PEG-TK@CA dressings had excellent wound-healing efficacy by promoting an early inflammatory response and subsequent M2 macrophage polarization in the wound-healing process.Conclusion: In conclusion, these findings indicated that SP@ZIF-8-PEG-TK@CA dressings might be useful for wound dressing applications in the clinic.Keywords: substance P, metal-organic framework, nanoparticles, hydrogel, wound healing
format	article
author	Zhu Y Yao Z Liu Y Zhang W Geng L Ni T
author_facet	Zhu Y Yao Z Liu Y Zhang W Geng L Ni T
author_sort	Zhu Y
title	Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications
title_short	Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications
title_full	Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications
title_fullStr	Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications
title_full_unstemmed	Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications
title_sort	incorporation of ros-responsive substance p-loaded zeolite imidazolate framework-8 nanoparticles into a ca2+-cross-linked alginate/pectin hydrogel for wound dressing applications
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/5791cedbbaa445a99742bc2c291643dc
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Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications

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