Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo

Xueyang Fang, Kwok-Ho Lui, Shiying Li, Wai-Sum Lo, Xin Li, Yanjuan Gu, Wing-tak Wong Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, People’s Republic of ChinaCorrespondence: Yanjuan Gu; Wing-tak WongDepa...

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Autores principales:	Fang X, Lui KH, Li S, Lo WS, Li X, Gu Y, Wong W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	gold-nanocage nanocomposites nir pa imaging synergistic therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/57927fb2684944bfab22a6d07c3d06f4
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spelling	oai:doaj.org-article:57927fb2684944bfab22a6d07c3d06f42021-12-02T13:47:45ZMultifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo1178-2013https://doaj.org/article/57927fb2684944bfab22a6d07c3d06f42020-12-01T00:00:00Zhttps://www.dovepress.com/multifunctional-nanotheranostic-gold-nanocageselenium-core-shell-for-p-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xueyang Fang, Kwok-Ho Lui, Shiying Li, Wai-Sum Lo, Xin Li, Yanjuan Gu, Wing-tak Wong Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, People’s Republic of ChinaCorrespondence: Yanjuan Gu; Wing-tak WongDepartment of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, People’s Republic of ChinaTel +852 3400 8858; +852 3400 8789Fax +852 2364 9932Email yanjuan.gu@polyu.edu.hk; w.t.wong@polyu.edu.hkIntroduction: Cancer theragnosis involving cancer diagnosis and targeted therapy simultaneously in one integrated system would be a promising solution of cancer treatment. Herein, a convenient and practical cancer theragnosis agent was constructed by combining gold nanocages (AuNCs) covered with selenium and a chitosan (CS) shell (AuNCs/Se) to incorporate the anti-cancer drug doxorubicin (DOX) as a multifunctional targeting nanocomposite (AuNCs/DOX@Se-iRGD) for photoacoustic imaging (PAI)-guided chemo-photothermal synergistic therapy that contributes to enhanced anti-cancer efficacy. The novel design of AuNCs/DOX@Se-iRGD gives the nanocomposite two outstanding properties: (1) AuNCs, with excellent LSPR property in the NIR region, act as a contrast agent for enhanced PAI and photothermal therapy (PTT); (2) Se acts as an anti-cancer nanoagent and drug delivery cargo.Methods: The photothermal performance of these nanocomposites was evaluated in different concentrations with laser powder densities. These nanocomposites were also incubated in pH 5.3, 6.5, 7.4 PBS and NIR laser to study their drug release ability. The cellular uptake was studied by measuring the Se and Au concentrations inside the cells using inductively coupled plasma-mass spectrometry (ICP-MS). Besides, in vitro and in vivo anti-tumor activity were carried out by cytotoxicity assay MTT and tumor model nude mice, respectively. As for imaging, the PA value and images of these nanocomposites accumulated in the tumor site were sequentially collected at specific time points for 48 h.Results and Discussion: The prepared AuNCs/DOX@Se-iRGD showed excellent biocompatibility and physiological stability in different media. In vivo results indicated that the targeting nanocomposite presented the strongest contrast-enhanced PAI signals, which could provide contour and location information of tumor, 24 h after intravenous injection. Likewise, the combined treatment of chemo- and photothermal synergistic therapy significantly inhibited tumor growth when compared with the two treatments carried out separately and showed negligible acute toxicity to the major organs.Conclusion: This study demonstrates that AuNCs/DOX@Se-iRGD has great prospect to become a multifunctional anti-tumor nanosystem for PAI-guided chemo- and photothermal synergistic therapy.Keywords: gold nanocage, nanocomposites, NIR, PA imaging, synergistic therapyFang XLui KHLi SLo WSLi XGu YWong WDove Medical Pressarticlegold-nanocagenanocompositesnirpa imagingsynergistic therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 10271-10284 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold-nanocage nanocomposites nir pa imaging synergistic therapy Medicine (General) R5-920
spellingShingle	gold-nanocage nanocomposites nir pa imaging synergistic therapy Medicine (General) R5-920 Fang X Lui KH Li S Lo WS Li X Gu Y Wong W Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo
description	Xueyang Fang, Kwok-Ho Lui, Shiying Li, Wai-Sum Lo, Xin Li, Yanjuan Gu, Wing-tak Wong Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, People’s Republic of ChinaCorrespondence: Yanjuan Gu; Wing-tak WongDepartment of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, People’s Republic of ChinaTel +852 3400 8858; +852 3400 8789Fax +852 2364 9932Email yanjuan.gu@polyu.edu.hk; w.t.wong@polyu.edu.hkIntroduction: Cancer theragnosis involving cancer diagnosis and targeted therapy simultaneously in one integrated system would be a promising solution of cancer treatment. Herein, a convenient and practical cancer theragnosis agent was constructed by combining gold nanocages (AuNCs) covered with selenium and a chitosan (CS) shell (AuNCs/Se) to incorporate the anti-cancer drug doxorubicin (DOX) as a multifunctional targeting nanocomposite (AuNCs/DOX@Se-iRGD) for photoacoustic imaging (PAI)-guided chemo-photothermal synergistic therapy that contributes to enhanced anti-cancer efficacy. The novel design of AuNCs/DOX@Se-iRGD gives the nanocomposite two outstanding properties: (1) AuNCs, with excellent LSPR property in the NIR region, act as a contrast agent for enhanced PAI and photothermal therapy (PTT); (2) Se acts as an anti-cancer nanoagent and drug delivery cargo.Methods: The photothermal performance of these nanocomposites was evaluated in different concentrations with laser powder densities. These nanocomposites were also incubated in pH 5.3, 6.5, 7.4 PBS and NIR laser to study their drug release ability. The cellular uptake was studied by measuring the Se and Au concentrations inside the cells using inductively coupled plasma-mass spectrometry (ICP-MS). Besides, in vitro and in vivo anti-tumor activity were carried out by cytotoxicity assay MTT and tumor model nude mice, respectively. As for imaging, the PA value and images of these nanocomposites accumulated in the tumor site were sequentially collected at specific time points for 48 h.Results and Discussion: The prepared AuNCs/DOX@Se-iRGD showed excellent biocompatibility and physiological stability in different media. In vivo results indicated that the targeting nanocomposite presented the strongest contrast-enhanced PAI signals, which could provide contour and location information of tumor, 24 h after intravenous injection. Likewise, the combined treatment of chemo- and photothermal synergistic therapy significantly inhibited tumor growth when compared with the two treatments carried out separately and showed negligible acute toxicity to the major organs.Conclusion: This study demonstrates that AuNCs/DOX@Se-iRGD has great prospect to become a multifunctional anti-tumor nanosystem for PAI-guided chemo- and photothermal synergistic therapy.Keywords: gold nanocage, nanocomposites, NIR, PA imaging, synergistic therapy
format	article
author	Fang X Lui KH Li S Lo WS Li X Gu Y Wong W
author_facet	Fang X Lui KH Li S Lo WS Li X Gu Y Wong W
author_sort	Fang X
title	Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo
title_short	Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo
title_full	Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo
title_fullStr	Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo
title_full_unstemmed	Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo
title_sort	multifunctional nanotheranostic gold nanocage/selenium core-shell for pai-guided chemo-photothermal synergistic therapy in vivo
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/57927fb2684944bfab22a6d07c3d06f4
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_version_	1718392497210654720

Multifunctional Nanotheranostic Gold Nanocage/Selenium Core-Shell for PAI-Guided Chemo-Photothermal Synergistic Therapy in vivo

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