Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level

Protein Carbonic Anhydrase IX (CA IX), which is expressed in various hypoxic solid tumors in order to maintain proper pH, is also related to cancer cell adhesion, invasion, and metastasis processes. Here, we investigated whether CA IX inhibition by a highly CA IX selective agent benzenesulfonamide V...

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Autores principales:	Agne Janoniene, Linas Mazutis, Daumantas Matulis, Vilma Petrikaite
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carbonic anhydrase IX single cell migration breast cancer sulfonamide microfluidic system Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/579aaccde4884c30b746cc687a0197b9
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spelling	oai:doaj.org-article:579aaccde4884c30b746cc687a0197b92021-11-11T17:03:07ZInhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level10.3390/ijms2221115711422-00671661-6596https://doaj.org/article/579aaccde4884c30b746cc687a0197b92021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11571https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Protein Carbonic Anhydrase IX (CA IX), which is expressed in various hypoxic solid tumors in order to maintain proper pH, is also related to cancer cell adhesion, invasion, and metastasis processes. Here, we investigated whether CA IX inhibition by a highly CA IX selective agent benzenesulfonamide VD11-4-2 triggers changes in individual cell motility. We seeded breast cancer cells on an extracellular matrix-coated glass-bottomed dish and in a microfluidic device with a gradient flow of epidermal growth factor (EGF), tracked individual cell movement, calculated their migration speeds, and/or followed movement direction. Our results showed that the inhibitor VD11-4-2 decreased the speed of CA IX positive breast cancer cells by 20–26% while not affecting non-cancerous cell migration. The inhibitor suppressed the cell migration velocity increment and hindered cells from reaching their maximum speed. VD11-4-2 also reduced CA IX, expressing cell movement towards the growth factor as a chemoattractant. Such a single cell-based migration assay enabled the comprehensive investigation of the cell motility and revealed that VD11-4-2 shows the ability to suppress breast cancer cell migration at a lower concentration than previously tested CA IX inhibitors.Agne JanonieneLinas MazutisDaumantas MatulisVilma PetrikaiteMDPI AGarticlecarbonic anhydrase IXsingle cell migrationbreast cancersulfonamidemicrofluidic systemBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11571, p 11571 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbonic anhydrase IX single cell migration breast cancer sulfonamide microfluidic system Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	carbonic anhydrase IX single cell migration breast cancer sulfonamide microfluidic system Biology (General) QH301-705.5 Chemistry QD1-999 Agne Janoniene Linas Mazutis Daumantas Matulis Vilma Petrikaite Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
description	Protein Carbonic Anhydrase IX (CA IX), which is expressed in various hypoxic solid tumors in order to maintain proper pH, is also related to cancer cell adhesion, invasion, and metastasis processes. Here, we investigated whether CA IX inhibition by a highly CA IX selective agent benzenesulfonamide VD11-4-2 triggers changes in individual cell motility. We seeded breast cancer cells on an extracellular matrix-coated glass-bottomed dish and in a microfluidic device with a gradient flow of epidermal growth factor (EGF), tracked individual cell movement, calculated their migration speeds, and/or followed movement direction. Our results showed that the inhibitor VD11-4-2 decreased the speed of CA IX positive breast cancer cells by 20–26% while not affecting non-cancerous cell migration. The inhibitor suppressed the cell migration velocity increment and hindered cells from reaching their maximum speed. VD11-4-2 also reduced CA IX, expressing cell movement towards the growth factor as a chemoattractant. Such a single cell-based migration assay enabled the comprehensive investigation of the cell motility and revealed that VD11-4-2 shows the ability to suppress breast cancer cell migration at a lower concentration than previously tested CA IX inhibitors.
format	article
author	Agne Janoniene Linas Mazutis Daumantas Matulis Vilma Petrikaite
author_facet	Agne Janoniene Linas Mazutis Daumantas Matulis Vilma Petrikaite
author_sort	Agne Janoniene
title	Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
title_short	Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
title_full	Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
title_fullStr	Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
title_full_unstemmed	Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level
title_sort	inhibition of carbonic anhydrase ix suppresses breast cancer cell motility at the single-cell level
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/579aaccde4884c30b746cc687a0197b9
work_keys_str_mv	AT agnejanoniene inhibitionofcarbonicanhydraseixsuppressesbreastcancercellmotilityatthesinglecelllevel AT linasmazutis inhibitionofcarbonicanhydraseixsuppressesbreastcancercellmotilityatthesinglecelllevel AT daumantasmatulis inhibitionofcarbonicanhydraseixsuppressesbreastcancercellmotilityatthesinglecelllevel AT vilmapetrikaite inhibitionofcarbonicanhydraseixsuppressesbreastcancercellmotilityatthesinglecelllevel
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Inhibition of Carbonic Anhydrase IX Suppresses Breast Cancer Cell Motility at the Single-Cell Level

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