Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection

ABSTRACT Most DNA viruses associate with, and reorganize, nuclear domain 10 (ND10) bodies upon entry into the host nucleus. In this study, we examine the roles of the ND10 components PML, Sp100, and Daxx in the establishment of human papillomavirus type 18 (HPV18) infection of primary human keratino...

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Autores principales:	Wesley H. Stepp, Jordan M. Meyers, Alison A. McBride
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/579dbd214bd5406e970c60d4b9dce74d
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spelling	oai:doaj.org-article:579dbd214bd5406e970c60d4b9dce74d2021-11-15T15:42:32ZSp100 Provides Intrinsic Immunity against Human Papillomavirus Infection10.1128/mBio.00845-132150-7511https://doaj.org/article/579dbd214bd5406e970c60d4b9dce74d2013-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00845-13https://doaj.org/toc/2150-7511ABSTRACT Most DNA viruses associate with, and reorganize, nuclear domain 10 (ND10) bodies upon entry into the host nucleus. In this study, we examine the roles of the ND10 components PML, Sp100, and Daxx in the establishment of human papillomavirus type 18 (HPV18) infection of primary human keratinocytes. HPV18 DNA or HPV18 quasivirus was introduced into primary human keratinocytes depleted of each ND10 protein by small interfering RNA technology, and genome establishment was determined by using a quantitative immortalization assay and measurements of viral transcription and DNA replication. Keratinocyte depletion of Sp100 resulted in a substantial increase in the number of HPV18-immortalized colonies and a corresponding increase in viral transcription and DNA replication. However, Sp100 repressed viral transcription and replication only during the initial stages of viral establishment, suggesting that Sp100 acts as a repressor of incoming HPV DNA. IMPORTANCE The intrinsic immune system provides a first-line defense against invading pathogens. Host cells contain nuclear bodies (ND10) that are important for antiviral defense, yet many DNA viruses localize here upon cell entry. However, viruses also disrupt, reorganize, and modify individual components of the bodies. In this study, we show that one of the ND10 components, Sp100, limits the infection of human skin cells by human papillomavirus (HPV). HPVs are important pathogens that cause many types of infection of the cutaneous and mucosal epithelium and are the causative agents of several human cancers. Understanding how host cells counteract HPV infection could provide insight into antimicrobial therapies that could limit initial infection.Wesley H. SteppJordan M. MeyersAlison A. McBrideAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 6 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Wesley H. Stepp Jordan M. Meyers Alison A. McBride Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection
description	ABSTRACT Most DNA viruses associate with, and reorganize, nuclear domain 10 (ND10) bodies upon entry into the host nucleus. In this study, we examine the roles of the ND10 components PML, Sp100, and Daxx in the establishment of human papillomavirus type 18 (HPV18) infection of primary human keratinocytes. HPV18 DNA or HPV18 quasivirus was introduced into primary human keratinocytes depleted of each ND10 protein by small interfering RNA technology, and genome establishment was determined by using a quantitative immortalization assay and measurements of viral transcription and DNA replication. Keratinocyte depletion of Sp100 resulted in a substantial increase in the number of HPV18-immortalized colonies and a corresponding increase in viral transcription and DNA replication. However, Sp100 repressed viral transcription and replication only during the initial stages of viral establishment, suggesting that Sp100 acts as a repressor of incoming HPV DNA. IMPORTANCE The intrinsic immune system provides a first-line defense against invading pathogens. Host cells contain nuclear bodies (ND10) that are important for antiviral defense, yet many DNA viruses localize here upon cell entry. However, viruses also disrupt, reorganize, and modify individual components of the bodies. In this study, we show that one of the ND10 components, Sp100, limits the infection of human skin cells by human papillomavirus (HPV). HPVs are important pathogens that cause many types of infection of the cutaneous and mucosal epithelium and are the causative agents of several human cancers. Understanding how host cells counteract HPV infection could provide insight into antimicrobial therapies that could limit initial infection.
format	article
author	Wesley H. Stepp Jordan M. Meyers Alison A. McBride
author_facet	Wesley H. Stepp Jordan M. Meyers Alison A. McBride
author_sort	Wesley H. Stepp
title	Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection
title_short	Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection
title_full	Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection
title_fullStr	Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection
title_full_unstemmed	Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection
title_sort	sp100 provides intrinsic immunity against human papillomavirus infection
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/579dbd214bd5406e970c60d4b9dce74d
work_keys_str_mv	AT wesleyhstepp sp100providesintrinsicimmunityagainsthumanpapillomavirusinfection AT jordanmmeyers sp100providesintrinsicimmunityagainsthumanpapillomavirusinfection AT alisonamcbride sp100providesintrinsicimmunityagainsthumanpapillomavirusinfection
_version_	1718427585191346176

Sp100 Provides Intrinsic Immunity against Human Papillomavirus Infection

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