In vivo Imaging of Retina and Choroid in Guinea Pigs

Purpose: To evaluate the feasibility of in-vivo imaging of the retina and choroid using spectral domain optical coherence tomography (OCT) in guinea pigs.Methods: The study included 19 pigmented guinea pigs (age: 3–4 weeks) which underwent sonographic axial length measurements and OCT imaging. At st...

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Autores principales:	Li Dong, Yi Fan Li, Xue Jiang, Yin Jun Lan, Lei Shao, Jost B. Jonas, Wen Bin Wei
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	in vivo imaging OCT retina repeatability reproducibility Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/579f6c0007014aa2b1b903c4cd2cc553
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spelling	oai:doaj.org-article:579f6c0007014aa2b1b903c4cd2cc5532021-12-02T09:16:31ZIn vivo Imaging of Retina and Choroid in Guinea Pigs2296-858X10.3389/fmed.2021.730494https://doaj.org/article/579f6c0007014aa2b1b903c4cd2cc5532021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.730494/fullhttps://doaj.org/toc/2296-858XPurpose: To evaluate the feasibility of in-vivo imaging of the retina and choroid using spectral domain optical coherence tomography (OCT) in guinea pigs.Methods: The study included 19 pigmented guinea pigs (age: 3–4 weeks) which underwent sonographic axial length measurements and OCT imaging. At study end, the animals were sacrificed and histomorphometric examinations of the retina and choroid were performed. We assessed the reproducibility of the OCT measurements and compared in-vivo measurements to histomorphometric data.Results: The mean thickness of the retina and choroid near the optic nerve head was 175.6 ± 25.8 and 63.4 ± 16.5 μm, respectively, and mean Bruch's membrane opening (BMO) diameter was 831 ± 121 μm. The intra-observer comparison of measurements of retinal thickness (intraclass correlation coefficient (ICC) = 0.92, 95% CI: 0.86–0.96; P < 0.001), choroidal thickness (ICC = 0.92, 95% CI: 0.86–0.96; P < 0.001), and BMO diameter (ICC = 0.92, 95% CI: 0.86–0.96; P < 0.001) showed a high correlation. A high agreement was present also for the inter-observer reproducibility of the measurements of retinal thickness (Pearson correlation coefficient (R) = 0.98; P < 0.001), choroidal thickness (R = 0.96; P < 0.001), and BMO diameter (R = 0.98; P < 0.001). The Bland-Altman plots showed that 2.6% (1/38), 5.3% (2/38), and 7.9% (3/38) of the measurement points of retinal thickness, choroidal thickness and BMO diameter, respectively, were located outside of the 95% limits of agreement. The OCT-based thickness measurements of retina and choroid were significantly higher than those measured by histomorphometry (both P-values <0.01).Conclusion: OCT-based in-vivo morphometric imaging of the retina and choroid in guinea pigs is feasible with an acceptable intra-observer repeatability and inter-observer reproducibility.Li DongYi Fan LiXue JiangYin Jun LanLei ShaoJost B. JonasJost B. JonasJost B. JonasWen Bin WeiFrontiers Media S.A.articlein vivo imagingOCTretinarepeatabilityreproducibilityMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	in vivo imaging OCT retina repeatability reproducibility Medicine (General) R5-920
spellingShingle	in vivo imaging OCT retina repeatability reproducibility Medicine (General) R5-920 Li Dong Yi Fan Li Xue Jiang Yin Jun Lan Lei Shao Jost B. Jonas Jost B. Jonas Jost B. Jonas Wen Bin Wei In vivo Imaging of Retina and Choroid in Guinea Pigs
description	Purpose: To evaluate the feasibility of in-vivo imaging of the retina and choroid using spectral domain optical coherence tomography (OCT) in guinea pigs.Methods: The study included 19 pigmented guinea pigs (age: 3–4 weeks) which underwent sonographic axial length measurements and OCT imaging. At study end, the animals were sacrificed and histomorphometric examinations of the retina and choroid were performed. We assessed the reproducibility of the OCT measurements and compared in-vivo measurements to histomorphometric data.Results: The mean thickness of the retina and choroid near the optic nerve head was 175.6 ± 25.8 and 63.4 ± 16.5 μm, respectively, and mean Bruch's membrane opening (BMO) diameter was 831 ± 121 μm. The intra-observer comparison of measurements of retinal thickness (intraclass correlation coefficient (ICC) = 0.92, 95% CI: 0.86–0.96; P < 0.001), choroidal thickness (ICC = 0.92, 95% CI: 0.86–0.96; P < 0.001), and BMO diameter (ICC = 0.92, 95% CI: 0.86–0.96; P < 0.001) showed a high correlation. A high agreement was present also for the inter-observer reproducibility of the measurements of retinal thickness (Pearson correlation coefficient (R) = 0.98; P < 0.001), choroidal thickness (R = 0.96; P < 0.001), and BMO diameter (R = 0.98; P < 0.001). The Bland-Altman plots showed that 2.6% (1/38), 5.3% (2/38), and 7.9% (3/38) of the measurement points of retinal thickness, choroidal thickness and BMO diameter, respectively, were located outside of the 95% limits of agreement. The OCT-based thickness measurements of retina and choroid were significantly higher than those measured by histomorphometry (both P-values <0.01).Conclusion: OCT-based in-vivo morphometric imaging of the retina and choroid in guinea pigs is feasible with an acceptable intra-observer repeatability and inter-observer reproducibility.
format	article
author	Li Dong Yi Fan Li Xue Jiang Yin Jun Lan Lei Shao Jost B. Jonas Jost B. Jonas Jost B. Jonas Wen Bin Wei
author_facet	Li Dong Yi Fan Li Xue Jiang Yin Jun Lan Lei Shao Jost B. Jonas Jost B. Jonas Jost B. Jonas Wen Bin Wei
author_sort	Li Dong
title	In vivo Imaging of Retina and Choroid in Guinea Pigs
title_short	In vivo Imaging of Retina and Choroid in Guinea Pigs
title_full	In vivo Imaging of Retina and Choroid in Guinea Pigs
title_fullStr	In vivo Imaging of Retina and Choroid in Guinea Pigs
title_full_unstemmed	In vivo Imaging of Retina and Choroid in Guinea Pigs
title_sort	in vivo imaging of retina and choroid in guinea pigs
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/579f6c0007014aa2b1b903c4cd2cc553
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_version_	1718398185839263744

In vivo Imaging of Retina and Choroid in Guinea Pigs

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