Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression

Abstract Peritoneal macrophages play a critical role in the control of infectious and inflammatory diseases. Although recent progress on murine peritoneal macrophages has revealed multiple aspects on their origin and mechanisms involved in their maintenance in this compartment, little is known on th...

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Autores principales:	Antonio José Ruiz-Alcaraz, Violeta Carmona-Martínez, María Tristán-Manzano, Francisco Machado-Linde, María Luisa Sánchez-Ferrer, Pilar García-Peñarrubia, María Martínez-Esparza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57a704814f6647368830708111e63ef6
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spelling	oai:doaj.org-article:57a704814f6647368830708111e63ef62021-12-02T15:07:56ZCharacterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression10.1038/s41598-018-30787-x2045-2322https://doaj.org/article/57a704814f6647368830708111e63ef62018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30787-xhttps://doaj.org/toc/2045-2322Abstract Peritoneal macrophages play a critical role in the control of infectious and inflammatory diseases. Although recent progress on murine peritoneal macrophages has revealed multiple aspects on their origin and mechanisms involved in their maintenance in this compartment, little is known on the characteristics of human peritoneal macrophages in homeostasis. Here, we have studied by flow cytometry several features of human peritoneal macrophages obtained from the peritoneal cavity of healthy women. Three peritoneal monocyte/macrophage subsets were established on the basis of CD14/CD16 expression (CD14++CD16−, CD14++CD16+ and CD14highCD16high), and analysis of CD11b, CD11c, CD40, CD62L, CD64, CD80, CD86, CD116, CD119, CD206, HLA-DR and Slan was carried out in each subpopulation. Intracellular expression of GATA6 and cytokines (pro-inflammatory IL-6 and TNF-α, anti-inflammatory IL-10) as well as their phagocytic/oxidative activities were also analyzed, in an attempt to identify genuine resident peritoneal macrophages. Results showed that human peritoneal macrophages are heterogeneous regarding their phenotype, cell complexity and functional abilities. A direct relationship of CD14/CD16 expression, intracellular content of GATA6, and activation/maturation markers like CD206 and HLA-DR, support that the CD14highCD16high subset represents the mature phenotype of steady-state human resident peritoneal macrophages. Furthermore, increased expression of CD14/CD16 is also related to the phagocytic activity.Antonio José Ruiz-AlcarazVioleta Carmona-MartínezMaría Tristán-ManzanoFrancisco Machado-LindeMaría Luisa Sánchez-FerrerPilar García-PeñarrubiaMaría Martínez-EsparzaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antonio José Ruiz-Alcaraz Violeta Carmona-Martínez María Tristán-Manzano Francisco Machado-Linde María Luisa Sánchez-Ferrer Pilar García-Peñarrubia María Martínez-Esparza Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression
description	Abstract Peritoneal macrophages play a critical role in the control of infectious and inflammatory diseases. Although recent progress on murine peritoneal macrophages has revealed multiple aspects on their origin and mechanisms involved in their maintenance in this compartment, little is known on the characteristics of human peritoneal macrophages in homeostasis. Here, we have studied by flow cytometry several features of human peritoneal macrophages obtained from the peritoneal cavity of healthy women. Three peritoneal monocyte/macrophage subsets were established on the basis of CD14/CD16 expression (CD14++CD16−, CD14++CD16+ and CD14highCD16high), and analysis of CD11b, CD11c, CD40, CD62L, CD64, CD80, CD86, CD116, CD119, CD206, HLA-DR and Slan was carried out in each subpopulation. Intracellular expression of GATA6 and cytokines (pro-inflammatory IL-6 and TNF-α, anti-inflammatory IL-10) as well as their phagocytic/oxidative activities were also analyzed, in an attempt to identify genuine resident peritoneal macrophages. Results showed that human peritoneal macrophages are heterogeneous regarding their phenotype, cell complexity and functional abilities. A direct relationship of CD14/CD16 expression, intracellular content of GATA6, and activation/maturation markers like CD206 and HLA-DR, support that the CD14highCD16high subset represents the mature phenotype of steady-state human resident peritoneal macrophages. Furthermore, increased expression of CD14/CD16 is also related to the phagocytic activity.
format	article
author	Antonio José Ruiz-Alcaraz Violeta Carmona-Martínez María Tristán-Manzano Francisco Machado-Linde María Luisa Sánchez-Ferrer Pilar García-Peñarrubia María Martínez-Esparza
author_facet	Antonio José Ruiz-Alcaraz Violeta Carmona-Martínez María Tristán-Manzano Francisco Machado-Linde María Luisa Sánchez-Ferrer Pilar García-Peñarrubia María Martínez-Esparza
author_sort	Antonio José Ruiz-Alcaraz
title	Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression
title_short	Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression
title_full	Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression
title_fullStr	Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression
title_full_unstemmed	Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression
title_sort	characterization of human peritoneal monocyte/macrophage subsets in homeostasis: phenotype, gata6, phagocytic/oxidative activities and cytokines expression
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/57a704814f6647368830708111e63ef6
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Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression

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