Recombinant expression and characterization of the cytoplasmic rice β-glucosidase Os1BGlu4.
The Os1BGlu4 β-glucosidase is the only glycoside hydrolase family 1 member in rice that is predicted to be localized in the cytoplasm. To characterize the biochemical function of rice Os1BGlu4, the Os1bglu4 cDNA was cloned and used to express a thioredoxin fusion protein in Escherichia coli. After r...
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Main Authors: | Chen Rouyi, Supaporn Baiya, Sang-Kyu Lee, Bancha Mahong, Jong-Seong Jeon, James R Ketudat-Cairns, Mariena Ketudat-Cairns |
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Format: | article |
Language: | EN |
Published: |
Public Library of Science (PLoS)
2014
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Online Access: | https://doaj.org/article/57a7e37b954740ab8fbf2e6c67e03259 |
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