Visualization of cortical projection neurons with retrograde TET-off lentiviral vector.
We are interested in identifying and characterizing various projection neurons that constitute the neocortical circuit. For this purpose, we developed a novel lentiviral vector that carries the tetracycline transactivator (tTA) and the transgene under the TET Responsive Element promoter (TRE) on a s...
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2012
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oai:doaj.org-article:57aa2bff172e4145975923a13a0557492021-11-18T08:13:07ZVisualization of cortical projection neurons with retrograde TET-off lentiviral vector.1932-620310.1371/journal.pone.0046157https://doaj.org/article/57aa2bff172e4145975923a13a0557492012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23071541/?tool=EBIhttps://doaj.org/toc/1932-6203We are interested in identifying and characterizing various projection neurons that constitute the neocortical circuit. For this purpose, we developed a novel lentiviral vector that carries the tetracycline transactivator (tTA) and the transgene under the TET Responsive Element promoter (TRE) on a single backbone. By pseudotyping such a vector with modified rabies G-protein, we were able to express palmitoylated-GFP (palGFP) or turboFP635 (RFP) in corticothalamic, corticocortical, and corticopontine neurons of mice. The high-level expression of the transgene achieved by the TET-Off system enabled us to observe characteristic elaboration of neuronal processes for each cell type. At higher magnification, we were able to observe fine structures such as boutons and spines as well. We also injected our retrograde TET-Off vector to the marmoset cortex and proved that it can be used to label the long-distance cortical connectivity of millimeter scale. In conclusion, our novel retrograde tracer provides an attractive option to investigate the morphologies of identified cortical projection neurons of various species.Akiya WatakabeShigeki KatoKazuto KobayashiMasafumi TakajiYuki NakagamiOsamu SadakaneMasanari OhtsukaHiroyuki HiokiTakeshi KanekoHiroyuki OkunoTakashi KawashimaHaruhiko BitoYoshihiro KitamuraTetsuo YamamoriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e46157 (2012) |
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Medicine R Science Q Akiya Watakabe Shigeki Kato Kazuto Kobayashi Masafumi Takaji Yuki Nakagami Osamu Sadakane Masanari Ohtsuka Hiroyuki Hioki Takeshi Kaneko Hiroyuki Okuno Takashi Kawashima Haruhiko Bito Yoshihiro Kitamura Tetsuo Yamamori Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. |
| description |
We are interested in identifying and characterizing various projection neurons that constitute the neocortical circuit. For this purpose, we developed a novel lentiviral vector that carries the tetracycline transactivator (tTA) and the transgene under the TET Responsive Element promoter (TRE) on a single backbone. By pseudotyping such a vector with modified rabies G-protein, we were able to express palmitoylated-GFP (palGFP) or turboFP635 (RFP) in corticothalamic, corticocortical, and corticopontine neurons of mice. The high-level expression of the transgene achieved by the TET-Off system enabled us to observe characteristic elaboration of neuronal processes for each cell type. At higher magnification, we were able to observe fine structures such as boutons and spines as well. We also injected our retrograde TET-Off vector to the marmoset cortex and proved that it can be used to label the long-distance cortical connectivity of millimeter scale. In conclusion, our novel retrograde tracer provides an attractive option to investigate the morphologies of identified cortical projection neurons of various species. |
| format |
article |
| author |
Akiya Watakabe Shigeki Kato Kazuto Kobayashi Masafumi Takaji Yuki Nakagami Osamu Sadakane Masanari Ohtsuka Hiroyuki Hioki Takeshi Kaneko Hiroyuki Okuno Takashi Kawashima Haruhiko Bito Yoshihiro Kitamura Tetsuo Yamamori |
| author_facet |
Akiya Watakabe Shigeki Kato Kazuto Kobayashi Masafumi Takaji Yuki Nakagami Osamu Sadakane Masanari Ohtsuka Hiroyuki Hioki Takeshi Kaneko Hiroyuki Okuno Takashi Kawashima Haruhiko Bito Yoshihiro Kitamura Tetsuo Yamamori |
| author_sort |
Akiya Watakabe |
| title |
Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. |
| title_short |
Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. |
| title_full |
Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. |
| title_fullStr |
Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. |
| title_full_unstemmed |
Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. |
| title_sort |
visualization of cortical projection neurons with retrograde tet-off lentiviral vector. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/57aa2bff172e4145975923a13a055749 |
| work_keys_str_mv |
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| _version_ |
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