Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease

Abstract Nonalcoholic steatohepatitis (NASH) is considered as a progressive form of nonalcoholic fatty liver disease (NAFLD). To distinguish NASH from nonalcoholic fatty liver (NAFL), we evaluated the diagnostic value of circulating miRNAs. Small RNA sequencing was performed on 12 NAFL patients and...

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Autores principales:	Tae Hyung Kim, Yoonseok Lee, Young-Sun Lee, Jeong-An Gim, Eunjung Ko, Sun Young Yim, Young Kul Jung, SeongHee Kang, Moon Young Kim, Hayeon Kim, Baek-hui Kim, Ji Hoon Kim, Yeon Seok Seo, Hyung Joon Yim, Jong Eun Yeon, Soon Ho Um, Kwan Soo Byun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57af42f491184a57a296eb4ffbc08ce0
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spelling	oai:doaj.org-article:57af42f491184a57a296eb4ffbc08ce02021-12-02T16:17:17ZCirculating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease10.1038/s41598-021-94115-62045-2322https://doaj.org/article/57af42f491184a57a296eb4ffbc08ce02021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94115-6https://doaj.org/toc/2045-2322Abstract Nonalcoholic steatohepatitis (NASH) is considered as a progressive form of nonalcoholic fatty liver disease (NAFLD). To distinguish NASH from nonalcoholic fatty liver (NAFL), we evaluated the diagnostic value of circulating miRNAs. Small RNA sequencing was performed on 12 NAFL patients and 12 NASH patients, and the miRNA expression was compared. After selecting miRNAs for the diagnosis of NASH, we analyzed the diagnostic accuracy of each miRNA and the combination of miRNAs. External validation was performed using quantitative reverse transcription PCR. Among the 2,588 miRNAs, 26 miRNAs significantly increased in the NASH group than in the NAFL group. Among the 26 elevated miRNAs in the NASH group, 8 miRNAs were selected, and in silico analysis was performed. Only four miRNAs (miR-21-5p, miR-151a-3p, miR-192-5p, and miR-4449) showed significant area under the receiver operating characteristic curve (AUC) values for NASH diagnosis. The combination of the four miRNAs showed satisfactory diagnostic accuracy for NASH (AUC 0.875; 95% CI 0.676–0.973). External validation revealed similar diagnostic accuracy for NASH (AUC 0.874; 95% CI 0.724–0.960). NASH represents significantly distinct miRNA expression profile compared with NAFL. The combination of serum circulating miRNAs can be used as a novel biomarker for the NASH diagnosis in NAFLD.Tae Hyung KimYoonseok LeeYoung-Sun LeeJeong-An GimEunjung KoSun Young YimYoung Kul JungSeongHee KangMoon Young KimHayeon KimBaek-hui KimJi Hoon KimYeon Seok SeoHyung Joon YimJong Eun YeonSoon Ho UmKwan Soo ByunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tae Hyung Kim Yoonseok Lee Young-Sun Lee Jeong-An Gim Eunjung Ko Sun Young Yim Young Kul Jung SeongHee Kang Moon Young Kim Hayeon Kim Baek-hui Kim Ji Hoon Kim Yeon Seok Seo Hyung Joon Yim Jong Eun Yeon Soon Ho Um Kwan Soo Byun Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease
description	Abstract Nonalcoholic steatohepatitis (NASH) is considered as a progressive form of nonalcoholic fatty liver disease (NAFLD). To distinguish NASH from nonalcoholic fatty liver (NAFL), we evaluated the diagnostic value of circulating miRNAs. Small RNA sequencing was performed on 12 NAFL patients and 12 NASH patients, and the miRNA expression was compared. After selecting miRNAs for the diagnosis of NASH, we analyzed the diagnostic accuracy of each miRNA and the combination of miRNAs. External validation was performed using quantitative reverse transcription PCR. Among the 2,588 miRNAs, 26 miRNAs significantly increased in the NASH group than in the NAFL group. Among the 26 elevated miRNAs in the NASH group, 8 miRNAs were selected, and in silico analysis was performed. Only four miRNAs (miR-21-5p, miR-151a-3p, miR-192-5p, and miR-4449) showed significant area under the receiver operating characteristic curve (AUC) values for NASH diagnosis. The combination of the four miRNAs showed satisfactory diagnostic accuracy for NASH (AUC 0.875; 95% CI 0.676–0.973). External validation revealed similar diagnostic accuracy for NASH (AUC 0.874; 95% CI 0.724–0.960). NASH represents significantly distinct miRNA expression profile compared with NAFL. The combination of serum circulating miRNAs can be used as a novel biomarker for the NASH diagnosis in NAFLD.
format	article
author	Tae Hyung Kim Yoonseok Lee Young-Sun Lee Jeong-An Gim Eunjung Ko Sun Young Yim Young Kul Jung SeongHee Kang Moon Young Kim Hayeon Kim Baek-hui Kim Ji Hoon Kim Yeon Seok Seo Hyung Joon Yim Jong Eun Yeon Soon Ho Um Kwan Soo Byun
author_facet	Tae Hyung Kim Yoonseok Lee Young-Sun Lee Jeong-An Gim Eunjung Ko Sun Young Yim Young Kul Jung SeongHee Kang Moon Young Kim Hayeon Kim Baek-hui Kim Ji Hoon Kim Yeon Seok Seo Hyung Joon Yim Jong Eun Yeon Soon Ho Um Kwan Soo Byun
author_sort	Tae Hyung Kim
title	Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease
title_short	Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease
title_full	Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease
title_fullStr	Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease
title_full_unstemmed	Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease
title_sort	circulating mirna is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/57af42f491184a57a296eb4ffbc08ce0
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Circulating miRNA is a useful diagnostic biomarker for nonalcoholic steatohepatitis in nonalcoholic fatty liver disease

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