Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.

Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrew P Liguori, Stephanie D Warrington, Jennifer L Ginther, Talima Pearson, Jolene Bowers, Mindy B Glass, Mark Mayo, Vanaporn Wuthiekanun, David Engelthaler, Sharon J Peacock, Bart J Currie, David M Wagner, Paul Keim, Apichai Tuanyok
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57b73549784849339c5b150d41ebe90c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:57b73549784849339c5b150d41ebe90c
record_format	dspace
spelling	oai:doaj.org-article:57b73549784849339c5b150d41ebe90c2021-11-18T07:32:04ZDiversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.1932-620310.1371/journal.pone.0029323https://doaj.org/article/57b73549784849339c5b150d41ebe90c2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22195045/?tool=EBIhttps://doaj.org/toc/1932-6203Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species.Andrew P LiguoriStephanie D WarringtonJennifer L GintherTalima PearsonJolene BowersMindy B GlassMark MayoVanaporn WuthiekanunDavid EngelthalerSharon J PeacockBart J CurrieDavid M WagnerPaul KeimApichai TuanyokPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e29323 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrew P Liguori Stephanie D Warrington Jennifer L Ginther Talima Pearson Jolene Bowers Mindy B Glass Mark Mayo Vanaporn Wuthiekanun David Engelthaler Sharon J Peacock Bart J Currie David M Wagner Paul Keim Apichai Tuanyok Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
description	Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species.
format	article
author	Andrew P Liguori Stephanie D Warrington Jennifer L Ginther Talima Pearson Jolene Bowers Mindy B Glass Mark Mayo Vanaporn Wuthiekanun David Engelthaler Sharon J Peacock Bart J Currie David M Wagner Paul Keim Apichai Tuanyok
author_facet	Andrew P Liguori Stephanie D Warrington Jennifer L Ginther Talima Pearson Jolene Bowers Mindy B Glass Mark Mayo Vanaporn Wuthiekanun David Engelthaler Sharon J Peacock Bart J Currie David M Wagner Paul Keim Apichai Tuanyok
author_sort	Andrew P Liguori
title	Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
title_short	Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
title_full	Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
title_fullStr	Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
title_full_unstemmed	Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
title_sort	diversity of 16s-23s rdna internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/57b73549784849339c5b150d41ebe90c
work_keys_str_mv	AT andrewpliguori diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT stephaniedwarrington diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT jenniferlginther diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT talimapearson diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT jolenebowers diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT mindybglass diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT markmayo diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT vanapornwuthiekanun diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT davidengelthaler diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT sharonjpeacock diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT bartjcurrie diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT davidmwagner diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT paulkeim diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors AT apichaituanyok diversityof16s23srdnainternaltranscribedspaceritsrevealsphylogeneticrelationshipsinburkholderiapseudomalleianditsnearneighbors
_version_	1718423285658550272

Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.

Ejemplares similares