V m -related extracellular potentials observed in red blood cells

Abstract Even in nonexcitable cells, the membrane potential V m is fundamental to cell function, with roles from ion channel regulation, development, to cancer metastasis. V m arises from transmembrane ion concentration gradients; standard models assume homogeneous extracellular and intracellular io...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Michael Pycraft Hughes, Emily J. Kruchek, Andrew D. Beale, Stephen J. Kitcatt, Sara Qureshi, Zachary P. Trott, Oriane Charbonnel, Paul A. Agbaje, Erin A. Henslee, Robert A. Dorey, Rebecca Lewis, Fatima H. Labeed
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57b96bc6687c43699ffc369c723f0e5f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:57b96bc6687c43699ffc369c723f0e5f
record_format	dspace
spelling	oai:doaj.org-article:57b96bc6687c43699ffc369c723f0e5f2021-12-02T17:18:22ZV m -related extracellular potentials observed in red blood cells10.1038/s41598-021-98102-92045-2322https://doaj.org/article/57b96bc6687c43699ffc369c723f0e5f2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98102-9https://doaj.org/toc/2045-2322Abstract Even in nonexcitable cells, the membrane potential V m is fundamental to cell function, with roles from ion channel regulation, development, to cancer metastasis. V m arises from transmembrane ion concentration gradients; standard models assume homogeneous extracellular and intracellular ion concentrations, and that V m only exists across the cell membrane and has no significance beyond it. Using red blood cells, we show that this is incorrect, or at least incomplete; V m is detectable beyond the cell surface, and modulating V m produces quantifiable and consistent changes in extracellular potential. Evidence strongly suggests this is due to capacitive coupling between V m and the electrical double layer, rather than molecular transporters. We show that modulating V m changes the extracellular ion composition, mimicking the behaviour if voltage-gated ion channels in non-excitable channels. We also observed V m -synchronised circadian rhythms in extracellular potential, with significant implications for cell–cell interactions and cardiovascular disease.Michael Pycraft HughesEmily J. KruchekAndrew D. BealeStephen J. KitcattSara QureshiZachary P. TrottOriane CharbonnelPaul A. AgbajeErin A. HensleeRobert A. DoreyRebecca LewisFatima H. LabeedNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael Pycraft Hughes Emily J. Kruchek Andrew D. Beale Stephen J. Kitcatt Sara Qureshi Zachary P. Trott Oriane Charbonnel Paul A. Agbaje Erin A. Henslee Robert A. Dorey Rebecca Lewis Fatima H. Labeed V m -related extracellular potentials observed in red blood cells
description	Abstract Even in nonexcitable cells, the membrane potential V m is fundamental to cell function, with roles from ion channel regulation, development, to cancer metastasis. V m arises from transmembrane ion concentration gradients; standard models assume homogeneous extracellular and intracellular ion concentrations, and that V m only exists across the cell membrane and has no significance beyond it. Using red blood cells, we show that this is incorrect, or at least incomplete; V m is detectable beyond the cell surface, and modulating V m produces quantifiable and consistent changes in extracellular potential. Evidence strongly suggests this is due to capacitive coupling between V m and the electrical double layer, rather than molecular transporters. We show that modulating V m changes the extracellular ion composition, mimicking the behaviour if voltage-gated ion channels in non-excitable channels. We also observed V m -synchronised circadian rhythms in extracellular potential, with significant implications for cell–cell interactions and cardiovascular disease.
format	article
author	Michael Pycraft Hughes Emily J. Kruchek Andrew D. Beale Stephen J. Kitcatt Sara Qureshi Zachary P. Trott Oriane Charbonnel Paul A. Agbaje Erin A. Henslee Robert A. Dorey Rebecca Lewis Fatima H. Labeed
author_facet	Michael Pycraft Hughes Emily J. Kruchek Andrew D. Beale Stephen J. Kitcatt Sara Qureshi Zachary P. Trott Oriane Charbonnel Paul A. Agbaje Erin A. Henslee Robert A. Dorey Rebecca Lewis Fatima H. Labeed
author_sort	Michael Pycraft Hughes
title	V m -related extracellular potentials observed in red blood cells
title_short	V m -related extracellular potentials observed in red blood cells
title_full	V m -related extracellular potentials observed in red blood cells
title_fullStr	V m -related extracellular potentials observed in red blood cells
title_full_unstemmed	V m -related extracellular potentials observed in red blood cells
title_sort	v m -related extracellular potentials observed in red blood cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/57b96bc6687c43699ffc369c723f0e5f
work_keys_str_mv	AT michaelpycrafthughes vmrelatedextracellularpotentialsobservedinredbloodcells AT emilyjkruchek vmrelatedextracellularpotentialsobservedinredbloodcells AT andrewdbeale vmrelatedextracellularpotentialsobservedinredbloodcells AT stephenjkitcatt vmrelatedextracellularpotentialsobservedinredbloodcells AT saraqureshi vmrelatedextracellularpotentialsobservedinredbloodcells AT zacharyptrott vmrelatedextracellularpotentialsobservedinredbloodcells AT orianecharbonnel vmrelatedextracellularpotentialsobservedinredbloodcells AT paulaagbaje vmrelatedextracellularpotentialsobservedinredbloodcells AT erinahenslee vmrelatedextracellularpotentialsobservedinredbloodcells AT robertadorey vmrelatedextracellularpotentialsobservedinredbloodcells AT rebeccalewis vmrelatedextracellularpotentialsobservedinredbloodcells AT fatimahlabeed vmrelatedextracellularpotentialsobservedinredbloodcells
_version_	1718381086304632832

V m -related extracellular potentials observed in red blood cells

Ejemplares similares