Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells

Supardi N, Sumarpo A, Sutejo R, Putra ABN, Kageyama N, Kawano M, Sugahara T. 2018. Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells. Biodiversitas 19: 330-335. Asam kandis (dried Garcinia xanthocymus Hook.f.e...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	NADYA SUPARDI, ANTON SUMARPO, RICHARD SUTEJO, AGUS BUDIAWAN NARO PUTRA, NAOKI KAGEYAMA, MIHO KAWANO, TAKUYA SUGAHARA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	asam kandis garcinia xanthocymus ige Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/57bbf33c17a14fa88229aa7b760a8872
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:57bbf33c17a14fa88229aa7b760a8872
record_format	dspace
spelling	oai:doaj.org-article:57bbf33c17a14fa88229aa7b760a88722021-11-16T14:01:05ZShort Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells1412-033X2085-472210.13057/biodiv/d190145https://doaj.org/article/57bbf33c17a14fa88229aa7b760a88722018-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2369https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Supardi N, Sumarpo A, Sutejo R, Putra ABN, Kageyama N, Kawano M, Sugahara T. 2018. Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells. Biodiversitas 19: 330-335. Asam kandis (dried Garcinia xanthocymus Hook.f.ex.T.Anderson) is popularly used as a spice in many Indonesian cuisines. Although Garcinia xanthocymus has been reported to possess various pharmacological properties, its immunologic functions have been poorly studied. Therefore, in this study, we aimed to determine immunoglobulin E (IgE) production-suppressing effect of Asam Kandis extract (AKE) using mouse hybridoma aDNP-Hy cells. AKE was prepared through 24 hours maceration in distilled water. IgE production-suppressing activity of AKE by aDNP-Hy cells was examined in time-wise manner. Cell viability and IgE mRNA expression were assessed using trypan blue assay and qRT-PCR, respectively. IgE production-suppressing activity of heat-treated (100°C) AKE was also examined. As a result, AKE significantly reduced IgE production by aDNP-Hy cells in a concentration-dependent manner both in 24 and 36 h incubation periods. Furthermore, AKE exhibited its IgE production-suppressing activity without any cytotoxicity and also significantly decreased IgE mRNA expression. Heat-treated AKE showed no IgE production-suppressing activity, indicating that the bioactive substances are heat labile. More interestingly, heat-treated AKE showed cytotoxic effect by aDNP-Hy cells. We showed that AKE exhibits IgE production-suppressing effect by aDNP-Hy cells. Further studies are necessary to discover underlying bioactive compounds of asam kandis and substance that responsible for the cytotoxic effect of heat-treated AKE.NADYA SUPARDIANTON SUMARPORICHARD SUTEJOAGUS BUDIAWAN NARO PUTRANAOKI KAGEYAMAMIHO KAWANOTAKUYA SUGAHARAMBI & UNS Soloarticleasam kandisgarcinia xanthocymusigeBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 1, Pp 330-335 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	asam kandis garcinia xanthocymus ige Biology (General) QH301-705.5
spellingShingle	asam kandis garcinia xanthocymus ige Biology (General) QH301-705.5 NADYA SUPARDI ANTON SUMARPO RICHARD SUTEJO AGUS BUDIAWAN NARO PUTRA NAOKI KAGEYAMA MIHO KAWANO TAKUYA SUGAHARA Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells
description	Supardi N, Sumarpo A, Sutejo R, Putra ABN, Kageyama N, Kawano M, Sugahara T. 2018. Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells. Biodiversitas 19: 330-335. Asam kandis (dried Garcinia xanthocymus Hook.f.ex.T.Anderson) is popularly used as a spice in many Indonesian cuisines. Although Garcinia xanthocymus has been reported to possess various pharmacological properties, its immunologic functions have been poorly studied. Therefore, in this study, we aimed to determine immunoglobulin E (IgE) production-suppressing effect of Asam Kandis extract (AKE) using mouse hybridoma aDNP-Hy cells. AKE was prepared through 24 hours maceration in distilled water. IgE production-suppressing activity of AKE by aDNP-Hy cells was examined in time-wise manner. Cell viability and IgE mRNA expression were assessed using trypan blue assay and qRT-PCR, respectively. IgE production-suppressing activity of heat-treated (100°C) AKE was also examined. As a result, AKE significantly reduced IgE production by aDNP-Hy cells in a concentration-dependent manner both in 24 and 36 h incubation periods. Furthermore, AKE exhibited its IgE production-suppressing activity without any cytotoxicity and also significantly decreased IgE mRNA expression. Heat-treated AKE showed no IgE production-suppressing activity, indicating that the bioactive substances are heat labile. More interestingly, heat-treated AKE showed cytotoxic effect by aDNP-Hy cells. We showed that AKE exhibits IgE production-suppressing effect by aDNP-Hy cells. Further studies are necessary to discover underlying bioactive compounds of asam kandis and substance that responsible for the cytotoxic effect of heat-treated AKE.
format	article
author	NADYA SUPARDI ANTON SUMARPO RICHARD SUTEJO AGUS BUDIAWAN NARO PUTRA NAOKI KAGEYAMA MIHO KAWANO TAKUYA SUGAHARA
author_facet	NADYA SUPARDI ANTON SUMARPO RICHARD SUTEJO AGUS BUDIAWAN NARO PUTRA NAOKI KAGEYAMA MIHO KAWANO TAKUYA SUGAHARA
author_sort	NADYA SUPARDI
title	Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells
title_short	Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells
title_full	Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells
title_fullStr	Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells
title_full_unstemmed	Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells
title_sort	short communication: ige production-suppressing effect of asam kandis (dried garcinia xantochymus) extracts by mouse hybridoma adnp-hy cells
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/57bbf33c17a14fa88229aa7b760a8872
work_keys_str_mv	AT nadyasupardi shortcommunicationigeproductionsuppressingeffectofasamkandisdriedgarciniaxantochymusextractsbymousehybridomaadnphycells AT antonsumarpo shortcommunicationigeproductionsuppressingeffectofasamkandisdriedgarciniaxantochymusextractsbymousehybridomaadnphycells AT richardsutejo shortcommunicationigeproductionsuppressingeffectofasamkandisdriedgarciniaxantochymusextractsbymousehybridomaadnphycells AT agusbudiawannaroputra shortcommunicationigeproductionsuppressingeffectofasamkandisdriedgarciniaxantochymusextractsbymousehybridomaadnphycells AT naokikageyama shortcommunicationigeproductionsuppressingeffectofasamkandisdriedgarciniaxantochymusextractsbymousehybridomaadnphycells AT mihokawano shortcommunicationigeproductionsuppressingeffectofasamkandisdriedgarciniaxantochymusextractsbymousehybridomaadnphycells AT takuyasugahara shortcommunicationigeproductionsuppressingeffectofasamkandisdriedgarciniaxantochymusextractsbymousehybridomaadnphycells
_version_	1718426421595996160

Short Communication: IgE production-suppressing effect of asam kandis (dried Garcinia xantochymus) extracts by mouse hybridoma aDNP-Hy Cells

Ejemplares similares