Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis

Qi Fei,* Xiaoyu Li,* Jisheng Lin,* Lingjia Yu,* Yong Yang* Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qi Fei Tel +86 10 6313 8353Fax +86 10 8391...

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Autores principales:	Fei Q, Li X, Lin J, Yu L, Yang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	male osteoporosis rna sequencing differentially expressed lncrnas differentially expressed genes osteoclast differentiation in vitro validation Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/57be0f4f380d4fdaadefaadc9414a0fc
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spelling	oai:doaj.org-article:57be0f4f380d4fdaadefaadc9414a0fc2021-12-02T11:49:04ZIdentification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis1178-1998https://doaj.org/article/57be0f4f380d4fdaadefaadc9414a0fc2020-09-01T00:00:00Zhttps://www.dovepress.com/identification-of-aberrantly-expressed-long-non-coding-rnas-and-nearby-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Qi Fei,* Xiaoyu Li,* Jisheng Lin,* Lingjia Yu,* Yong Yang* Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qi Fei Tel +86 10 6313 8353Fax +86 10 8391 1029Email spinefei@126.comPurpose: To investigate different expression profiles of long non-coding RNAs (lncRNAs) and mRNAs between male osteoporosis and normal control by high throughput RNA sequencing.Methods: We obtained the different expression profiles of long non-coding RNAs (lncRNAs) and mRNAs between male osteoporosis and normal control by high throughput RNA sequencing. Compared to normal control, we identified the differentially expressed genes (DEGs), differentially expressed lncRNAs (DElncRNAs) and the nearby targeted DEGs of DElncRNAs in male osteoporosis. Functional annotation was used to further study the functions of DEGs in male osteoporosis. The DElncRNAs–DEGs interaction network was constructed. One DElncRNA-nearby targeted DEG interaction pair of LINC02009-CCR2 was validated in vitro.Results: Totally, 3296 DEGs, 204 DElncRNAs and 168 DElncRNAs-nearby targeted DEGs pairs were obtained. The most significantly up-regulated and down-regulated DElncRNAs in male osteoporosis were Loc105372801 and KCNQ1OT1, respectively. Osteoclast differentiation and chemokine signaling pathway were significantly enriched pathways in male osteoporosis. Based on the DElncRNAs–DEGs interaction network in male osteoporosis, we obtained several interaction pairs including SNHG5-SYNCRIP-HBA1-HBB, HCG27-HLA-C, LINC02009-CCR2, and LOC101926887-IFIT1-IFIT2/IFIT3/IFIT5. The expression of LINC02009 and CCR2 was down-regulated in keeping with the RNA sequencing data.Conclusion: Identified DElncRNAs–DEGs interaction pairs may be involved in the development of male osteoporosis, which make a contribution to underlying the mechanism of male osteoporosis. Among which, the validated DElncRNAs-nearby targeted DEGs interaction pair of LINC02009-CCR2 may be important regulators in the development of male osteoporosis.Keywords: male osteoporosis, RNA sequencing, differentially expressed lncRNAs, differentially expressed genes, osteoclast differentiation, in vitro validationFei QLi XLin JYu LYang YDove Medical Pressarticlemale osteoporosisrna sequencingdifferentially expressed lncrnasdifferentially expressed genesosteoclast differentiationin vitro validationGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 15, Pp 1779-1792 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	male osteoporosis rna sequencing differentially expressed lncrnas differentially expressed genes osteoclast differentiation in vitro validation Geriatrics RC952-954.6
spellingShingle	male osteoporosis rna sequencing differentially expressed lncrnas differentially expressed genes osteoclast differentiation in vitro validation Geriatrics RC952-954.6 Fei Q Li X Lin J Yu L Yang Y Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis
description	Qi Fei,* Xiaoyu Li,* Jisheng Lin,* Lingjia Yu,* Yong Yang* Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qi Fei Tel +86 10 6313 8353Fax +86 10 8391 1029Email spinefei@126.comPurpose: To investigate different expression profiles of long non-coding RNAs (lncRNAs) and mRNAs between male osteoporosis and normal control by high throughput RNA sequencing.Methods: We obtained the different expression profiles of long non-coding RNAs (lncRNAs) and mRNAs between male osteoporosis and normal control by high throughput RNA sequencing. Compared to normal control, we identified the differentially expressed genes (DEGs), differentially expressed lncRNAs (DElncRNAs) and the nearby targeted DEGs of DElncRNAs in male osteoporosis. Functional annotation was used to further study the functions of DEGs in male osteoporosis. The DElncRNAs–DEGs interaction network was constructed. One DElncRNA-nearby targeted DEG interaction pair of LINC02009-CCR2 was validated in vitro.Results: Totally, 3296 DEGs, 204 DElncRNAs and 168 DElncRNAs-nearby targeted DEGs pairs were obtained. The most significantly up-regulated and down-regulated DElncRNAs in male osteoporosis were Loc105372801 and KCNQ1OT1, respectively. Osteoclast differentiation and chemokine signaling pathway were significantly enriched pathways in male osteoporosis. Based on the DElncRNAs–DEGs interaction network in male osteoporosis, we obtained several interaction pairs including SNHG5-SYNCRIP-HBA1-HBB, HCG27-HLA-C, LINC02009-CCR2, and LOC101926887-IFIT1-IFIT2/IFIT3/IFIT5. The expression of LINC02009 and CCR2 was down-regulated in keeping with the RNA sequencing data.Conclusion: Identified DElncRNAs–DEGs interaction pairs may be involved in the development of male osteoporosis, which make a contribution to underlying the mechanism of male osteoporosis. Among which, the validated DElncRNAs-nearby targeted DEGs interaction pair of LINC02009-CCR2 may be important regulators in the development of male osteoporosis.Keywords: male osteoporosis, RNA sequencing, differentially expressed lncRNAs, differentially expressed genes, osteoclast differentiation, in vitro validation
format	article
author	Fei Q Li X Lin J Yu L Yang Y
author_facet	Fei Q Li X Lin J Yu L Yang Y
author_sort	Fei Q
title	Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis
title_short	Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis
title_full	Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis
title_fullStr	Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis
title_full_unstemmed	Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis
title_sort	identification of aberrantly expressed long non-coding rnas and nearby targeted genes in male osteoporosis
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/57be0f4f380d4fdaadefaadc9414a0fc
work_keys_str_mv	AT feiq identificationofaberrantlyexpressedlongnoncodingrnasandnearbytargetedgenesinmaleosteoporosis AT lix identificationofaberrantlyexpressedlongnoncodingrnasandnearbytargetedgenesinmaleosteoporosis AT linj identificationofaberrantlyexpressedlongnoncodingrnasandnearbytargetedgenesinmaleosteoporosis AT yul identificationofaberrantlyexpressedlongnoncodingrnasandnearbytargetedgenesinmaleosteoporosis AT yangy identificationofaberrantlyexpressedlongnoncodingrnasandnearbytargetedgenesinmaleosteoporosis
_version_	1718395227096481792

Identification of Aberrantly Expressed Long Non-Coding RNAs and Nearby Targeted Genes in Male Osteoporosis

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