Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure

Abstract We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbation differential scanning calorimetry (PPC) that studies a system on the whole by giving access to...

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Autores principales:	J. Peters, J. Marion, F. J. Becher, M. Trapp, T. Gutberlet, D. J. Bicout, T. Heimburg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57c3499640cc4f67867d4c378b8b2703
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spelling	oai:doaj.org-article:57c3499640cc4f67867d4c378b8b27032021-12-02T11:52:15ZThermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure10.1038/s41598-017-15582-42045-2322https://doaj.org/article/57c3499640cc4f67867d4c378b8b27032017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15582-4https://doaj.org/toc/2045-2322Abstract We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbation differential scanning calorimetry (PPC) that studies a system on the whole by giving access to relevant thermodynamic quantities, and elastic incoherent neutron scattering (EINS) that probes local motions of a system at the atomic level by allowing extraction of dynamical parameters. PPC revealed that the volume expansion coefficient of DMPC and DMPC/Cholesterol samples with 13 and 25 mol% cholesterol is a linear function of the heat capacity measured by differential scanning calorimetry. Neutron backscattering spectroscopy showed that the mean square displacements of H atoms do exhibit an increase with temperature and a decrease under increasing pressure. Cholesterol added at concentrations of 25 and 50 mol% led to suppression of the main phase transition. Taking advantage of these results, the present study aims (i) to show that calorimetry and EINS using the Bicout and Zaccai model equally permit to get access to thermodynamic quantities characterizing pure DMPC and DMPC/cholesterol mixtures, thus directly confirming the theoretical method, and (ii) to validate our approach as function of temperature and of pressure, as both are equally important and complementary thermodynamic variables.J. PetersJ. MarionF. J. BecherM. TrappT. GutberletD. J. BicoutT. HeimburgNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. Peters J. Marion F. J. Becher M. Trapp T. Gutberlet D. J. Bicout T. Heimburg Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
description	Abstract We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbation differential scanning calorimetry (PPC) that studies a system on the whole by giving access to relevant thermodynamic quantities, and elastic incoherent neutron scattering (EINS) that probes local motions of a system at the atomic level by allowing extraction of dynamical parameters. PPC revealed that the volume expansion coefficient of DMPC and DMPC/Cholesterol samples with 13 and 25 mol% cholesterol is a linear function of the heat capacity measured by differential scanning calorimetry. Neutron backscattering spectroscopy showed that the mean square displacements of H atoms do exhibit an increase with temperature and a decrease under increasing pressure. Cholesterol added at concentrations of 25 and 50 mol% led to suppression of the main phase transition. Taking advantage of these results, the present study aims (i) to show that calorimetry and EINS using the Bicout and Zaccai model equally permit to get access to thermodynamic quantities characterizing pure DMPC and DMPC/cholesterol mixtures, thus directly confirming the theoretical method, and (ii) to validate our approach as function of temperature and of pressure, as both are equally important and complementary thermodynamic variables.
format	article
author	J. Peters J. Marion F. J. Becher M. Trapp T. Gutberlet D. J. Bicout T. Heimburg
author_facet	J. Peters J. Marion F. J. Becher M. Trapp T. Gutberlet D. J. Bicout T. Heimburg
author_sort	J. Peters
title	Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
title_short	Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
title_full	Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
title_fullStr	Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
title_full_unstemmed	Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
title_sort	thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/57c3499640cc4f67867d4c378b8b2703
work_keys_str_mv	AT jpeters thermodynamicsoflipidmultilamellarvesiclesinpresenceofsterolsathighhydrostaticpressure AT jmarion thermodynamicsoflipidmultilamellarvesiclesinpresenceofsterolsathighhydrostaticpressure AT fjbecher thermodynamicsoflipidmultilamellarvesiclesinpresenceofsterolsathighhydrostaticpressure AT mtrapp thermodynamicsoflipidmultilamellarvesiclesinpresenceofsterolsathighhydrostaticpressure AT tgutberlet thermodynamicsoflipidmultilamellarvesiclesinpresenceofsterolsathighhydrostaticpressure AT djbicout thermodynamicsoflipidmultilamellarvesiclesinpresenceofsterolsathighhydrostaticpressure AT theimburg thermodynamicsoflipidmultilamellarvesiclesinpresenceofsterolsathighhydrostaticpressure
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Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure

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