CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer

Abstract Gastric cancer is the third most common cause of cancer mortality, and the survival rate of stage IV advanced gastric cancer (AGC) patients with distant metastasis is very low. Thus, the detection and eradication of disseminated cancer cells by targeting cell surface molecules in AGC would...

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Autores principales:	Eun-Seok Choi, Hyunjin Kim, Hyung-Pyo Kim, Yongdoo Choi, Sung-Ho Goh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57c5306b483b4325a372f9761049980d
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spelling	oai:doaj.org-article:57c5306b483b4325a372f9761049980d2021-12-02T12:32:26ZCD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer10.1038/s41598-017-05247-72045-2322https://doaj.org/article/57c5306b483b4325a372f9761049980d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05247-7https://doaj.org/toc/2045-2322Abstract Gastric cancer is the third most common cause of cancer mortality, and the survival rate of stage IV advanced gastric cancer (AGC) patients with distant metastasis is very low. Thus, the detection and eradication of disseminated cancer cells by targeting cell surface molecules in AGC would improve patient survival. The hyaluronic acid receptor, CD44, has various isoforms generated by alternative splicing, and some isoforms are known to be correlated to gastric cancer. In this study, to find out the most appropriate CD44v for targeting AGC, we analysed the expression differences of CD44 isoforms at the mRNA level in stomach cancer cell lines as well as in 74 patients with AGC by using exon-specific qRT-PCR. Among the CD44v isoforms, CD44v8-10 was determined as the most promising biomarker for the development of theranostic agents of gastric cancer. Next, we synthesised the conjugate of anti-CD44v9 antibody with near-infrared fluorophore or photosensitiser, and then demonstrated its feasibility for target cell-specific imaging and photoimmunotherapy in gastric cancer. As a result, these conjugates have clearly demarcated the surface of CD44v8-10 expressing cancer cells and showed efficient phototoxic effects. Therefore, this study revealed that CD44v8-10 is the efficient theranostic biomarker to target disseminated cancer cells in AGC.Eun-Seok ChoiHyunjin KimHyung-Pyo KimYongdoo ChoiSung-Ho GohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eun-Seok Choi Hyunjin Kim Hyung-Pyo Kim Yongdoo Choi Sung-Ho Goh CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer
description	Abstract Gastric cancer is the third most common cause of cancer mortality, and the survival rate of stage IV advanced gastric cancer (AGC) patients with distant metastasis is very low. Thus, the detection and eradication of disseminated cancer cells by targeting cell surface molecules in AGC would improve patient survival. The hyaluronic acid receptor, CD44, has various isoforms generated by alternative splicing, and some isoforms are known to be correlated to gastric cancer. In this study, to find out the most appropriate CD44v for targeting AGC, we analysed the expression differences of CD44 isoforms at the mRNA level in stomach cancer cell lines as well as in 74 patients with AGC by using exon-specific qRT-PCR. Among the CD44v isoforms, CD44v8-10 was determined as the most promising biomarker for the development of theranostic agents of gastric cancer. Next, we synthesised the conjugate of anti-CD44v9 antibody with near-infrared fluorophore or photosensitiser, and then demonstrated its feasibility for target cell-specific imaging and photoimmunotherapy in gastric cancer. As a result, these conjugates have clearly demarcated the surface of CD44v8-10 expressing cancer cells and showed efficient phototoxic effects. Therefore, this study revealed that CD44v8-10 is the efficient theranostic biomarker to target disseminated cancer cells in AGC.
format	article
author	Eun-Seok Choi Hyunjin Kim Hyung-Pyo Kim Yongdoo Choi Sung-Ho Goh
author_facet	Eun-Seok Choi Hyunjin Kim Hyung-Pyo Kim Yongdoo Choi Sung-Ho Goh
author_sort	Eun-Seok Choi
title	CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer
title_short	CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer
title_full	CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer
title_fullStr	CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer
title_full_unstemmed	CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer
title_sort	cd44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/57c5306b483b4325a372f9761049980d
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CD44v8-10 as a potential theranostic biomarker for targeting disseminated cancer cells in advanced gastric cancer

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