Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)

Abstract Background Black pepper (Piper nigrum L.), an important and long-cultivated spice crop, is native to South India and grown in the tropics. Piperine is the main pungent and bioactive alkaloid in the berries of black pepper, but the molecular mechanism for piperine biosynthesis has not been d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuanhao Ding, Yuyuan Mao, Yi Cen, Lisong Hu, Yuefeng Su, Xuemin Ma, Lu Long, Haiyan Hu, Chaoyun Hao, Jie Luo
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Black pepper miRNA Secondary metabolism Alkaloids Piperine biosynthesis Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/57c9920020ed437e954578fe648001ac
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:57c9920020ed437e954578fe648001ac
record_format	dspace
spelling	oai:doaj.org-article:57c9920020ed437e954578fe648001ac2021-11-21T12:26:25ZSmall RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)10.1186/s12864-021-08154-41471-2164https://doaj.org/article/57c9920020ed437e954578fe648001ac2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08154-4https://doaj.org/toc/1471-2164Abstract Background Black pepper (Piper nigrum L.), an important and long-cultivated spice crop, is native to South India and grown in the tropics. Piperine is the main pungent and bioactive alkaloid in the berries of black pepper, but the molecular mechanism for piperine biosynthesis has not been determined. MicroRNAs (miRNAs), which are classical endogenous noncoding small RNAs, play important roles in regulating secondary metabolism in many species, but less is known regarding black pepper or piperine biosynthesis. Results To dissect the functions of miRNAs in secondary metabolism especially in piperine biosynthesis, 110 known miRNAs, 18 novel miRNAs and 1007 individual targets were identified from different tissues of black pepper by small RNA sequencing. qRT-PCR and 5′-RLM-RACE experiments were conducted to validate the reliability of the sequencing data and predicted targets. We found 3 miRNAs along with their targets including miR166-4CL, miR396-PER and miR397-CCR modules that are involved in piperine biosynthesis. Conclusion MiRNA regulation of secondary metabolism is a common phenomenon in plants. Our study revealed new miRNAs that regulate piperine biosynthesis, which are special alkaloids in the piper genus, and they might be useful for future piperine genetic improvement of black pepper.Yuanhao DingYuyuan MaoYi CenLisong HuYuefeng SuXuemin MaLu LongHaiyan HuChaoyun HaoJie LuoBMCarticleBlack peppermiRNASecondary metabolismAlkaloidsPiperine biosynthesisBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Black pepper miRNA Secondary metabolism Alkaloids Piperine biosynthesis Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Black pepper miRNA Secondary metabolism Alkaloids Piperine biosynthesis Biotechnology TP248.13-248.65 Genetics QH426-470 Yuanhao Ding Yuyuan Mao Yi Cen Lisong Hu Yuefeng Su Xuemin Ma Lu Long Haiyan Hu Chaoyun Hao Jie Luo Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)
description	Abstract Background Black pepper (Piper nigrum L.), an important and long-cultivated spice crop, is native to South India and grown in the tropics. Piperine is the main pungent and bioactive alkaloid in the berries of black pepper, but the molecular mechanism for piperine biosynthesis has not been determined. MicroRNAs (miRNAs), which are classical endogenous noncoding small RNAs, play important roles in regulating secondary metabolism in many species, but less is known regarding black pepper or piperine biosynthesis. Results To dissect the functions of miRNAs in secondary metabolism especially in piperine biosynthesis, 110 known miRNAs, 18 novel miRNAs and 1007 individual targets were identified from different tissues of black pepper by small RNA sequencing. qRT-PCR and 5′-RLM-RACE experiments were conducted to validate the reliability of the sequencing data and predicted targets. We found 3 miRNAs along with their targets including miR166-4CL, miR396-PER and miR397-CCR modules that are involved in piperine biosynthesis. Conclusion MiRNA regulation of secondary metabolism is a common phenomenon in plants. Our study revealed new miRNAs that regulate piperine biosynthesis, which are special alkaloids in the piper genus, and they might be useful for future piperine genetic improvement of black pepper.
format	article
author	Yuanhao Ding Yuyuan Mao Yi Cen Lisong Hu Yuefeng Su Xuemin Ma Lu Long Haiyan Hu Chaoyun Hao Jie Luo
author_facet	Yuanhao Ding Yuyuan Mao Yi Cen Lisong Hu Yuefeng Su Xuemin Ma Lu Long Haiyan Hu Chaoyun Hao Jie Luo
author_sort	Yuanhao Ding
title	Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)
title_short	Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)
title_full	Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)
title_fullStr	Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)
title_full_unstemmed	Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)
title_sort	small rna sequencing reveals various micrornas involved in piperine biosynthesis in black pepper (piper nigrum l.)
publisher	BMC
publishDate	2021
url	https://doaj.org/article/57c9920020ed437e954578fe648001ac
work_keys_str_mv	AT yuanhaoding smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT yuyuanmao smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT yicen smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT lisonghu smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT yuefengsu smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT xueminma smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT lulong smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT haiyanhu smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT chaoyunhao smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml AT jieluo smallrnasequencingrevealsvariousmicrornasinvolvedinpiperinebiosynthesisinblackpepperpipernigruml
_version_	1718419007762071552

Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.)

Ejemplares similares