Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation

Vin&iacute;cius Raphael de Almeida Borges, Alice Simon, Adrian Ricardo Cuello Sena, L&uacute;cio Mendes Cabral, Val&eacute;ria Pereira de Sousa Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Background: Topical a...

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Autores principales:	Borges VRA, Simon A, Sena ARC, Cabral LM, de Sousa VP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/57cd6f76629d4f68ac147a855590dac2
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spelling	oai:doaj.org-article:57cd6f76629d4f68ac147a855590dac22021-12-02T02:28:08ZNanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation1176-91141178-2013https://doaj.org/article/57cd6f76629d4f68ac147a855590dac22013-02-01T00:00:00Zhttp://www.dovepress.com/nanoemulsion-containing-dapsone-for-topical-administration-a-study-of--a12169https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Vin&iacute;cius Raphael de Almeida Borges, Alice Simon, Adrian Ricardo Cuello Sena, L&uacute;cio Mendes Cabral, Val&eacute;ria Pereira de Sousa Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Background: Topical administration of dapsone can be an alternative route for treatment of leprosy and can also provide new therapeutic applications for an established drug. However, the physicochemical properties of dapsone make it difficult to incorporate into conventional formulations. The current study was directed toward developing a stable nanoemulsion that contains dapsone which can be adapted for topical use. Methods: Nanoemulsions were prepared using isopropyl myristate or n-methyl-pyrrolidone as the oil phase, and characterized according to their mean droplet size, conductivity, refractive index, pH, drug content, and stability. The in vitro release of dapsone and its ability to permeate the epidermis were also evaluated. Results: Physicochemical characterization demonstrated that nanosystems were formed, which had a uniform droplet distribution and a pH compatible with the skin surface. Use of n-methyl-pyrrolidone provided a greater nanoemulsion region and higher solubilization of dapsone, and increased the in vitro release rate when compared with a nanoemulsion prepared using isopropyl myristate. However, use of isopropyl myristate promoted an increase in in vitro epidermal permeation that followed the Higuchi model. This demonstrates the ability of a nanosystem to influence permeation of dapsone through the skin barrier. Furthermore, the nanoemulsions developed and evaluated here had ideal physicochemical stability over a 3-month period. Conclusion: Incorporation of dapsone into a nanoemulsion may be a promising system for enabling topical delivery of dapsone, while minimizing skin permeation, for the treatment of acne. The method developed here used isopropyl myristate as the oil phase, and promoted permeation of dapsone through the skin barrier for the treatment of leprosy upon use of n-methyl-pyrrolidone as the oil phase. Keywords: dapsone, nanoemulsion, topical, release, permeationBorges VRASimon ASena ARCCabral LMde Sousa VPDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 535-544 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Borges VRA Simon A Sena ARC Cabral LM de Sousa VP Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation
description	Vin&iacute;cius Raphael de Almeida Borges, Alice Simon, Adrian Ricardo Cuello Sena, L&uacute;cio Mendes Cabral, Val&eacute;ria Pereira de Sousa Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Background: Topical administration of dapsone can be an alternative route for treatment of leprosy and can also provide new therapeutic applications for an established drug. However, the physicochemical properties of dapsone make it difficult to incorporate into conventional formulations. The current study was directed toward developing a stable nanoemulsion that contains dapsone which can be adapted for topical use. Methods: Nanoemulsions were prepared using isopropyl myristate or n-methyl-pyrrolidone as the oil phase, and characterized according to their mean droplet size, conductivity, refractive index, pH, drug content, and stability. The in vitro release of dapsone and its ability to permeate the epidermis were also evaluated. Results: Physicochemical characterization demonstrated that nanosystems were formed, which had a uniform droplet distribution and a pH compatible with the skin surface. Use of n-methyl-pyrrolidone provided a greater nanoemulsion region and higher solubilization of dapsone, and increased the in vitro release rate when compared with a nanoemulsion prepared using isopropyl myristate. However, use of isopropyl myristate promoted an increase in in vitro epidermal permeation that followed the Higuchi model. This demonstrates the ability of a nanosystem to influence permeation of dapsone through the skin barrier. Furthermore, the nanoemulsions developed and evaluated here had ideal physicochemical stability over a 3-month period. Conclusion: Incorporation of dapsone into a nanoemulsion may be a promising system for enabling topical delivery of dapsone, while minimizing skin permeation, for the treatment of acne. The method developed here used isopropyl myristate as the oil phase, and promoted permeation of dapsone through the skin barrier for the treatment of leprosy upon use of n-methyl-pyrrolidone as the oil phase. Keywords: dapsone, nanoemulsion, topical, release, permeation
format	article
author	Borges VRA Simon A Sena ARC Cabral LM de Sousa VP
author_facet	Borges VRA Simon A Sena ARC Cabral LM de Sousa VP
author_sort	Borges VRA
title	Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation
title_short	Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation
title_full	Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation
title_fullStr	Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation
title_full_unstemmed	Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation
title_sort	nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/57cd6f76629d4f68ac147a855590dac2
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Nanoemulsion containing dapsone for topical administration: a study of in vitro release and epidermal permeation

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