Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats

Abstract In diabetes, retinal dysfunctions exist prior to clinically detectable vasculopathy, however the pathology behind these functional deficits is still not fully established. Previously, our group published a detailed study on the retinal histopathology of type 1 diabetic (T1D) rat model, wher...

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Autores principales:	Klaudia Szabó, Anna Énzsöly, Bulcsú Dékány, Arnold Szabó, Rozina I. Hajdú, Tamás Radovits, Csaba Mátyás, Attila Oláh, Lenke K. Laurik, Gábor M. Somfai, Béla Merkely, Ágoston Szél, Ákos Lukáts
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57d0d283bf724bdb8426c37685ee16e6
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spelling	oai:doaj.org-article:57d0d283bf724bdb8426c37685ee16e62021-12-02T12:30:19ZHistological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats10.1038/s41598-017-09068-62045-2322https://doaj.org/article/57d0d283bf724bdb8426c37685ee16e62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09068-6https://doaj.org/toc/2045-2322Abstract In diabetes, retinal dysfunctions exist prior to clinically detectable vasculopathy, however the pathology behind these functional deficits is still not fully established. Previously, our group published a detailed study on the retinal histopathology of type 1 diabetic (T1D) rat model, where specific alterations were detected. Although the majority of human diabetic patients have type 2 diabetes (T2D), similar studies on T2D models are practically absent. To fill this gap, we examined Zucker Diabetic Fatty (ZDF) rats - a model for T2D - by immunohistochemistry at the age of 32 weeks. Glial reactivity was observed in all diabetic specimens, accompanied by an increase in the number of microglia cells. Prominent outer segment degeneration was detectable with changes in cone opsin expression pattern, without a decrease in the number of labelled elements. The immunoreactivity of AII amacrine cells was markedly decreased and changes were detectable in the number and staining of some other amacrine cell subtypes, while most other cells examined did not show any major alterations. Overall, the retinal histology of ZDF rats shows a surprising similarity to T1D rats indicating that despite the different evolution of the disease, the neuroretinal cells affected are the same in both subtypes of diabetes.Klaudia SzabóAnna ÉnzsölyBulcsú DékányArnold SzabóRozina I. HajdúTamás RadovitsCsaba MátyásAttila OláhLenke K. LaurikGábor M. SomfaiBéla MerkelyÁgoston SzélÁkos LukátsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Klaudia Szabó Anna Énzsöly Bulcsú Dékány Arnold Szabó Rozina I. Hajdú Tamás Radovits Csaba Mátyás Attila Oláh Lenke K. Laurik Gábor M. Somfai Béla Merkely Ágoston Szél Ákos Lukáts Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats
description	Abstract In diabetes, retinal dysfunctions exist prior to clinically detectable vasculopathy, however the pathology behind these functional deficits is still not fully established. Previously, our group published a detailed study on the retinal histopathology of type 1 diabetic (T1D) rat model, where specific alterations were detected. Although the majority of human diabetic patients have type 2 diabetes (T2D), similar studies on T2D models are practically absent. To fill this gap, we examined Zucker Diabetic Fatty (ZDF) rats - a model for T2D - by immunohistochemistry at the age of 32 weeks. Glial reactivity was observed in all diabetic specimens, accompanied by an increase in the number of microglia cells. Prominent outer segment degeneration was detectable with changes in cone opsin expression pattern, without a decrease in the number of labelled elements. The immunoreactivity of AII amacrine cells was markedly decreased and changes were detectable in the number and staining of some other amacrine cell subtypes, while most other cells examined did not show any major alterations. Overall, the retinal histology of ZDF rats shows a surprising similarity to T1D rats indicating that despite the different evolution of the disease, the neuroretinal cells affected are the same in both subtypes of diabetes.
format	article
author	Klaudia Szabó Anna Énzsöly Bulcsú Dékány Arnold Szabó Rozina I. Hajdú Tamás Radovits Csaba Mátyás Attila Oláh Lenke K. Laurik Gábor M. Somfai Béla Merkely Ágoston Szél Ákos Lukáts
author_facet	Klaudia Szabó Anna Énzsöly Bulcsú Dékány Arnold Szabó Rozina I. Hajdú Tamás Radovits Csaba Mátyás Attila Oláh Lenke K. Laurik Gábor M. Somfai Béla Merkely Ágoston Szél Ákos Lukáts
author_sort	Klaudia Szabó
title	Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats
title_short	Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats
title_full	Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats
title_fullStr	Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats
title_full_unstemmed	Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats
title_sort	histological evaluation of diabetic neurodegeneration in the retina of zucker diabetic fatty (zdf) rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/57d0d283bf724bdb8426c37685ee16e6
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Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats

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