REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS

Abstract. Enhanced transendothelial migration of leukocytes during infection and inflammation occurs due to increased expression of ICAM-1 and VCAM-1 adherence molecules of the immunoglobulin superfamily, and increased secretion of chemokines by endothelial cells. Our previous in vitro studies with...

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Autores principales:	E. A. Starikova, A. M. Lebedeva, N. A. Aktureeva, L. A. Burova, I. S. Freidlin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	thp-1 cells transendothelial migration streptococcus pyogenes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/57d422a75a694f798f6ec63fa5f76b9c
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spelling	oai:doaj.org-article:57d422a75a694f798f6ec63fa5f76b9c2021-11-18T08:03:43ZREGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS1563-06252313-741X10.15789/1563-0625-2013-5-457-464https://doaj.org/article/57d422a75a694f798f6ec63fa5f76b9c2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/662https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Enhanced transendothelial migration of leukocytes during infection and inflammation occurs due to increased expression of ICAM-1 and VCAM-1 adherence molecules of the immunoglobulin superfamily, and increased secretion of chemokines by endothelial cells. Our previous in vitro studies with human monocytic THP-1 cells showed that some components of Streptococcus pyogenes caused a sufficient enhancement of transendothelial cell migration. However, expression levels of adhesion molecules remained unchanged on endothelial cells. The purpose of the work was to study mechanisms underlying the enhanced transendothelial migration of THP-1 cells treated with supernatante of streptococcal cells (SSC). SSC exhibited chemoattractive activity towards THP-1 cells. SSC increased chemotaxis and transmigration of THP-1 cells. Meanwhile, a 24-hour preincubation of THP-1 cells with SSC caused suppression of the both effects, being accompanied by decreased level of Focal Adhesion Kinase phosphorylation. Preincubation of THP-1 cells with pertussis toxin did reduce the transmigration intensity in presence of the SSC down to the level of spontaneous migration. These results suggest that increased transmigration of THP-1 cells under the influence of SSC may be determined by its chemoattractive action upon the migrating cells.E. A. StarikovaA. M. LebedevaN. A. AktureevaL. A. BurovaI. S. FreidlinSPb RAACIarticlethp-1 cellstransendothelial migrationstreptococcus pyogenesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 5, Pp 457-464 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	thp-1 cells transendothelial migration streptococcus pyogenes Immunologic diseases. Allergy RC581-607
spellingShingle	thp-1 cells transendothelial migration streptococcus pyogenes Immunologic diseases. Allergy RC581-607 E. A. Starikova A. M. Lebedeva N. A. Aktureeva L. A. Burova I. S. Freidlin REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS
description	Abstract. Enhanced transendothelial migration of leukocytes during infection and inflammation occurs due to increased expression of ICAM-1 and VCAM-1 adherence molecules of the immunoglobulin superfamily, and increased secretion of chemokines by endothelial cells. Our previous in vitro studies with human monocytic THP-1 cells showed that some components of Streptococcus pyogenes caused a sufficient enhancement of transendothelial cell migration. However, expression levels of adhesion molecules remained unchanged on endothelial cells. The purpose of the work was to study mechanisms underlying the enhanced transendothelial migration of THP-1 cells treated with supernatante of streptococcal cells (SSC). SSC exhibited chemoattractive activity towards THP-1 cells. SSC increased chemotaxis and transmigration of THP-1 cells. Meanwhile, a 24-hour preincubation of THP-1 cells with SSC caused suppression of the both effects, being accompanied by decreased level of Focal Adhesion Kinase phosphorylation. Preincubation of THP-1 cells with pertussis toxin did reduce the transmigration intensity in presence of the SSC down to the level of spontaneous migration. These results suggest that increased transmigration of THP-1 cells under the influence of SSC may be determined by its chemoattractive action upon the migrating cells.
format	article
author	E. A. Starikova A. M. Lebedeva N. A. Aktureeva L. A. Burova I. S. Freidlin
author_facet	E. A. Starikova A. M. Lebedeva N. A. Aktureeva L. A. Burova I. S. Freidlin
author_sort	E. A. Starikova
title	REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS
title_short	REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS
title_full	REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS
title_fullStr	REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS
title_full_unstemmed	REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS
title_sort	regulatory mechanisms of human monocytic thp-1 cells transendothelial migration induced by streptococcus pyogenes components
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/57d422a75a694f798f6ec63fa5f76b9c
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REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS

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