Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation

Abstract Bagging is an efficient method to improve fruit colour development. This work reported a transcriptome analysis using bagging-treated red Chinese sand pear peels. In total, 8,870 differentially expressed genes were further analysed by a weighted gene co-expression network analysis and early...

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Autores principales:	Songling Bai, Yongwang Sun, Minjie Qian, Fengxia Yang, Junbei Ni, Ruiyan Tao, Lin Li, Qun Shu, Dong Zhang, Yuanwen Teng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57d455864db643a2ae76c721d96f949c
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spelling	oai:doaj.org-article:57d455864db643a2ae76c721d96f949c2021-12-02T12:30:46ZTranscriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation10.1038/s41598-017-00069-z2045-2322https://doaj.org/article/57d455864db643a2ae76c721d96f949c2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00069-zhttps://doaj.org/toc/2045-2322Abstract Bagging is an efficient method to improve fruit colour development. This work reported a transcriptome analysis using bagging-treated red Chinese sand pear peels. In total, 8,870 differentially expressed genes were further analysed by a weighted gene co-expression network analysis and early-, middle- and late light-responsive genes were identified. An annotation analysis revealed several pathways involved in the different responsive stages. The presence of LONG HYPOCOTLY 5, CRY-DASH and a CONSTANS-like transcription factors among the early light-responsive genes indicated the pivotal role of light, especially blue light, in the biological changes that occurred after bag removal. Other light-responsive transcription factors were also identified from the three light-responsive stages. In addition, the light-responsive pattern of anthocyanin biosynthetic genes differed among the biosynthetic steps. Although yeast-one hybrid assay showed that most of the structural genes were regulated by PpMYB10, their different temporal expressive pattern suggested that besides PpMYB10, other light-responsive transcriptional factors were also involved in the regulation of anthocyanin biosynthesis. In summary, our transcriptome analysis provides knowledge of the transcriptional regulatory network operating during light responses, which results in anthocyanin accumulation and other significant physiological changes in red Chinese sand pear peels after bag removal.Songling BaiYongwang SunMinjie QianFengxia YangJunbei NiRuiyan TaoLin LiQun ShuDong ZhangYuanwen TengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Songling Bai Yongwang Sun Minjie Qian Fengxia Yang Junbei Ni Ruiyan Tao Lin Li Qun Shu Dong Zhang Yuanwen Teng Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation
description	Abstract Bagging is an efficient method to improve fruit colour development. This work reported a transcriptome analysis using bagging-treated red Chinese sand pear peels. In total, 8,870 differentially expressed genes were further analysed by a weighted gene co-expression network analysis and early-, middle- and late light-responsive genes were identified. An annotation analysis revealed several pathways involved in the different responsive stages. The presence of LONG HYPOCOTLY 5, CRY-DASH and a CONSTANS-like transcription factors among the early light-responsive genes indicated the pivotal role of light, especially blue light, in the biological changes that occurred after bag removal. Other light-responsive transcription factors were also identified from the three light-responsive stages. In addition, the light-responsive pattern of anthocyanin biosynthetic genes differed among the biosynthetic steps. Although yeast-one hybrid assay showed that most of the structural genes were regulated by PpMYB10, their different temporal expressive pattern suggested that besides PpMYB10, other light-responsive transcriptional factors were also involved in the regulation of anthocyanin biosynthesis. In summary, our transcriptome analysis provides knowledge of the transcriptional regulatory network operating during light responses, which results in anthocyanin accumulation and other significant physiological changes in red Chinese sand pear peels after bag removal.
format	article
author	Songling Bai Yongwang Sun Minjie Qian Fengxia Yang Junbei Ni Ruiyan Tao Lin Li Qun Shu Dong Zhang Yuanwen Teng
author_facet	Songling Bai Yongwang Sun Minjie Qian Fengxia Yang Junbei Ni Ruiyan Tao Lin Li Qun Shu Dong Zhang Yuanwen Teng
author_sort	Songling Bai
title	Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation
title_short	Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation
title_full	Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation
title_fullStr	Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation
title_full_unstemmed	Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation
title_sort	transcriptome analysis of bagging-treated red chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/57d455864db643a2ae76c721d96f949c
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Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation

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