Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.

Ovarian high grade serous carcinoma (HGSC) is a lethal form of ovarian cancer (OVCA). In most cases it is detected at late stages as the symptoms are non-specific during early stages. Emerging information suggests that the oviductal fimbria is a site of origin of ovarian HGSC. Currently available te...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elizabeth A Paris, Janice M Bahr, Pincas Bitterman, Sanjib Basu, Jacques S Abramowicz, Animesh Barua
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57de66ce9e5e491b933141bb8bedee63
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:57de66ce9e5e491b933141bb8bedee63
record_format	dspace
spelling	oai:doaj.org-article:57de66ce9e5e491b933141bb8bedee632021-12-02T20:06:23ZIncidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.1932-620310.1371/journal.pone.0255007https://doaj.org/article/57de66ce9e5e491b933141bb8bedee632021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255007https://doaj.org/toc/1932-6203Ovarian high grade serous carcinoma (HGSC) is a lethal form of ovarian cancer (OVCA). In most cases it is detected at late stages as the symptoms are non-specific during early stages. Emerging information suggests that the oviductal fimbria is a site of origin of ovarian HGSC. Currently available tests cannot detect ovarian HGSC at early stage. The lack of a preclinical model with oviductal fimbria that develops spontaneous ovarian HGSC is a significant barrier to developing an early detection test for this disease. The goal of this study was to examine if the oviductal fimbria in hens is a site of origin of HGSC and whether it expresses several putative markers expressed in ovarian HGSC in patients. A total of 135 laying hens (4 years old) were selected from a flock using transvaginal ultrasound (TVUS) imaging, followed by euthanasia and gross examination for the presence of solid masses and ascites. Histological types of carcinomas were determined by hematoxylin and eosin staining. Expression of WT-1, mutant p53, CA-125, PAX2 and Ki67 in normal or malignant fimbriae or ovaries were examined using immunohistochemistry, immunoblotting and gene expression assays. This study detected tumors in oviductal fimbriae in hens and routine staining revealed ovarian HGSC-like microscopic features in these tumors. These tumors showed similarities to ovarian HGSC in patients in expressing several markers. Compared with normal fimbriae, intensities of expression of WT-1, mutant p53, CA-125, and Ki67 were significantly (P<0.05) higher in fimbrial tumors. In contrast, expression of PAX2 decreased gradually as the tumor progressed to late stages. The patterns of expression of these markers were similar to those in ovarian HGSC patients. Thus, tumors of the oviductal fimbria in hens may offer a preclinical model to study different aspects of spontaneous ovarian HGSC in women including its early detection.Elizabeth A ParisJanice M BahrPincas BittermanSanjib BasuJacques S AbramowiczAnimesh BaruaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255007 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elizabeth A Paris Janice M Bahr Pincas Bitterman Sanjib Basu Jacques S Abramowicz Animesh Barua Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
description	Ovarian high grade serous carcinoma (HGSC) is a lethal form of ovarian cancer (OVCA). In most cases it is detected at late stages as the symptoms are non-specific during early stages. Emerging information suggests that the oviductal fimbria is a site of origin of ovarian HGSC. Currently available tests cannot detect ovarian HGSC at early stage. The lack of a preclinical model with oviductal fimbria that develops spontaneous ovarian HGSC is a significant barrier to developing an early detection test for this disease. The goal of this study was to examine if the oviductal fimbria in hens is a site of origin of HGSC and whether it expresses several putative markers expressed in ovarian HGSC in patients. A total of 135 laying hens (4 years old) were selected from a flock using transvaginal ultrasound (TVUS) imaging, followed by euthanasia and gross examination for the presence of solid masses and ascites. Histological types of carcinomas were determined by hematoxylin and eosin staining. Expression of WT-1, mutant p53, CA-125, PAX2 and Ki67 in normal or malignant fimbriae or ovaries were examined using immunohistochemistry, immunoblotting and gene expression assays. This study detected tumors in oviductal fimbriae in hens and routine staining revealed ovarian HGSC-like microscopic features in these tumors. These tumors showed similarities to ovarian HGSC in patients in expressing several markers. Compared with normal fimbriae, intensities of expression of WT-1, mutant p53, CA-125, and Ki67 were significantly (P<0.05) higher in fimbrial tumors. In contrast, expression of PAX2 decreased gradually as the tumor progressed to late stages. The patterns of expression of these markers were similar to those in ovarian HGSC patients. Thus, tumors of the oviductal fimbria in hens may offer a preclinical model to study different aspects of spontaneous ovarian HGSC in women including its early detection.
format	article
author	Elizabeth A Paris Janice M Bahr Pincas Bitterman Sanjib Basu Jacques S Abramowicz Animesh Barua
author_facet	Elizabeth A Paris Janice M Bahr Pincas Bitterman Sanjib Basu Jacques S Abramowicz Animesh Barua
author_sort	Elizabeth A Paris
title	Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
title_short	Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
title_full	Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
title_fullStr	Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
title_full_unstemmed	Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
title_sort	incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/57de66ce9e5e491b933141bb8bedee63
work_keys_str_mv	AT elizabethaparis incidenceofmalignanttransformationintheoviductalfimbriainlayinghensapreclinicalmodelofspontaneousovariancancer AT janicembahr incidenceofmalignanttransformationintheoviductalfimbriainlayinghensapreclinicalmodelofspontaneousovariancancer AT pincasbitterman incidenceofmalignanttransformationintheoviductalfimbriainlayinghensapreclinicalmodelofspontaneousovariancancer AT sanjibbasu incidenceofmalignanttransformationintheoviductalfimbriainlayinghensapreclinicalmodelofspontaneousovariancancer AT jacquessabramowicz incidenceofmalignanttransformationintheoviductalfimbriainlayinghensapreclinicalmodelofspontaneousovariancancer AT animeshbarua incidenceofmalignanttransformationintheoviductalfimbriainlayinghensapreclinicalmodelofspontaneousovariancancer
_version_	1718375360966426624

Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.

Ejemplares similares