Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response

Atopic dermatitis (AD) leads to skin barrier abnormalities and immune dysfunction. As the topical steroids commonly used to treat AD have side effects from long-term use, research into safer treatments for AD is greatly needed. The medicinal herb Gardenia jasminoides improves AD symptoms via skin ba...

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Autores principales:	Sun Haeng Park, Seol Jang, Ho Kyoung Kim
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Atopic dermatitis Gardenia jasminoides Dermatophagoides farina Immune regulation IgE Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/57deb292b44d494889cd67716b474229
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spelling	oai:doaj.org-article:57deb292b44d494889cd67716b4742292021-11-28T04:28:04ZGardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response0753-332210.1016/j.biopha.2021.112344https://doaj.org/article/57deb292b44d494889cd67716b4742292022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011288https://doaj.org/toc/0753-3322Atopic dermatitis (AD) leads to skin barrier abnormalities and immune dysfunction. As the topical steroids commonly used to treat AD have side effects from long-term use, research into safer treatments for AD is greatly needed. The medicinal herb Gardenia jasminoides improves AD symptoms via skin barrier activation and T helper 2-mediated immune response regulation. Crocin, a bioactive component within the extract, is dispensible for its restorative effects. As such, this work explored the effects of Gardenia jasminoides extract without crocin (GjexCr) on AD symptoms in a DfE-induced AD model in 6-week-old male NC/Nga mice (25.0 ± 0.25 g, n = 10 each, 6 groups). Using histological and behavioral assays, the effects of GjexCr on dermatitis scores, scratching behavior, skin barrier activation, and serum levels of IgE, chemokines, and cytokines were analyzed. In addition, the major components from the GjexCr extract were analyzed by high-performance liquid chromatography and validated in the AD model. GjexCr reduced ear thickness due to hyperkeratosis, dermal thickening, and scratching behavior and restored dermatitis scores in AD-induced mice. GjexCr administration also decreased inflammation and mast cell infiltration, as well as modulated skin barrier recovery by upregulating the production of epidermal proteins. Moreover, GjexCr administration attenuated imbalanced immune responses. Furthermore, geniposide, the main component of GjexCr, improved AD symptoms in DfE-treated NC/Nga mice. Thus, GjexCr could be a suitable treatment for protecting the skin barrier in AD-like skin lesions and a potential therapy for AD.Sun Haeng ParkSeol JangHo Kyoung KimElsevierarticleAtopic dermatitisGardenia jasminoidesDermatophagoides farinaImmune regulationIgETherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112344- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Atopic dermatitis Gardenia jasminoides Dermatophagoides farina Immune regulation IgE Therapeutics. Pharmacology RM1-950
spellingShingle	Atopic dermatitis Gardenia jasminoides Dermatophagoides farina Immune regulation IgE Therapeutics. Pharmacology RM1-950 Sun Haeng Park Seol Jang Ho Kyoung Kim Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response
description	Atopic dermatitis (AD) leads to skin barrier abnormalities and immune dysfunction. As the topical steroids commonly used to treat AD have side effects from long-term use, research into safer treatments for AD is greatly needed. The medicinal herb Gardenia jasminoides improves AD symptoms via skin barrier activation and T helper 2-mediated immune response regulation. Crocin, a bioactive component within the extract, is dispensible for its restorative effects. As such, this work explored the effects of Gardenia jasminoides extract without crocin (GjexCr) on AD symptoms in a DfE-induced AD model in 6-week-old male NC/Nga mice (25.0 ± 0.25 g, n = 10 each, 6 groups). Using histological and behavioral assays, the effects of GjexCr on dermatitis scores, scratching behavior, skin barrier activation, and serum levels of IgE, chemokines, and cytokines were analyzed. In addition, the major components from the GjexCr extract were analyzed by high-performance liquid chromatography and validated in the AD model. GjexCr reduced ear thickness due to hyperkeratosis, dermal thickening, and scratching behavior and restored dermatitis scores in AD-induced mice. GjexCr administration also decreased inflammation and mast cell infiltration, as well as modulated skin barrier recovery by upregulating the production of epidermal proteins. Moreover, GjexCr administration attenuated imbalanced immune responses. Furthermore, geniposide, the main component of GjexCr, improved AD symptoms in DfE-treated NC/Nga mice. Thus, GjexCr could be a suitable treatment for protecting the skin barrier in AD-like skin lesions and a potential therapy for AD.
format	article
author	Sun Haeng Park Seol Jang Ho Kyoung Kim
author_facet	Sun Haeng Park Seol Jang Ho Kyoung Kim
author_sort	Sun Haeng Park
title	Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response
title_short	Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response
title_full	Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response
title_fullStr	Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response
title_full_unstemmed	Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response
title_sort	gardenia jasminoides extract ameliorates dfe-induced atopic dermatitis in mice through restoration of barrier function and t-helper 2-mediated immune response
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/57deb292b44d494889cd67716b474229
work_keys_str_mv	AT sunhaengpark gardeniajasminoidesextractamelioratesdfeinducedatopicdermatitisinmicethroughrestorationofbarrierfunctionandthelper2mediatedimmuneresponse AT seoljang gardeniajasminoidesextractamelioratesdfeinducedatopicdermatitisinmicethroughrestorationofbarrierfunctionandthelper2mediatedimmuneresponse AT hokyoungkim gardeniajasminoidesextractamelioratesdfeinducedatopicdermatitisinmicethroughrestorationofbarrierfunctionandthelper2mediatedimmuneresponse
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Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response

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