Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery

Abstract Aims To analyze changes in oropharynx microbiota composition after receiving induced chemotherapy followed by surgery for hypopharyngeal squamous cell carcinoma (HPSCC) patients. Methods Clinical data and swab samples of 38 HPSCC patients (HPSCC group) and 30 patients with benign disease (c...

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Autores principales:	Hui-Ching Lau, Chi-Yao Hsueh, Hongli Gong, Ji Sun, Hui-Ying Huang, Ming Zhang, Liang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Hypopharyngeal carcinoma Induced chemotherapy 16S rRNA sequencing Fusobacterium Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/57decd9bd49142948bc867eb34371d50
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spelling	oai:doaj.org-article:57decd9bd49142948bc867eb34371d502021-11-14T12:08:46ZOropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery10.1186/s12866-021-02362-41471-2180https://doaj.org/article/57decd9bd49142948bc867eb34371d502021-11-01T00:00:00Zhttps://doi.org/10.1186/s12866-021-02362-4https://doaj.org/toc/1471-2180Abstract Aims To analyze changes in oropharynx microbiota composition after receiving induced chemotherapy followed by surgery for hypopharyngeal squamous cell carcinoma (HPSCC) patients. Methods Clinical data and swab samples of 38 HPSCC patients (HPSCC group) and 30 patients with benign disease (control group, CG) were enrolled in the study. HPSCC group was stratified into two groups: induced chemotherapy group (IC) of 10 patients and non-induced chemotherapy group (nIC) of 28 patients. The microbiota from oropharyngeal membrane was analyzed through 16S rRNA sequencing. Results Alpha-diversity (Shannon and Ace indexes) and weighted UniFrac based beta-diversity severely decreased in the HPSCC group when compared with CG. In pre-operative comparisons, PCoA and NMDS analyses showed microbial structures in the IC group were more similar to CG than nIC. Both IC group and nIC group yielded significantly diverse post-operative communities in contrast to their pre-operative counterparts, evident by the decrease in genera Veillonella and Fusobacterium and increase in genera Streptococcus and Gemella. Given that post-operative oropharynx microbiota showed no difference between IC and nIC groups, the IC group showed less accumulation in anaerobic communities. The abundance of genera Fusobacterium, Parvimonas, Actinomyces were enhanced in the advanced stages (III/IV). Conclusions Oropharynx microbiota in the HPSCC group presents dysbiosis with low diversity and abundance. Induced chemotherapy is beneficial in adjusting the oropharynx microbial environment leading to fewer amounts of anaerobe accumulation after operation. Higher amounts of Fusobacterium in advanced stages (III/IV) may influence the progression of HPSCC.Hui-Ching LauChi-Yao HsuehHongli GongJi SunHui-Ying HuangMing ZhangLiang ZhouBMCarticleHypopharyngeal carcinomaInduced chemotherapy16S rRNA sequencingFusobacteriumMicrobiologyQR1-502ENBMC Microbiology, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hypopharyngeal carcinoma Induced chemotherapy 16S rRNA sequencing Fusobacterium Microbiology QR1-502
spellingShingle	Hypopharyngeal carcinoma Induced chemotherapy 16S rRNA sequencing Fusobacterium Microbiology QR1-502 Hui-Ching Lau Chi-Yao Hsueh Hongli Gong Ji Sun Hui-Ying Huang Ming Zhang Liang Zhou Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
description	Abstract Aims To analyze changes in oropharynx microbiota composition after receiving induced chemotherapy followed by surgery for hypopharyngeal squamous cell carcinoma (HPSCC) patients. Methods Clinical data and swab samples of 38 HPSCC patients (HPSCC group) and 30 patients with benign disease (control group, CG) were enrolled in the study. HPSCC group was stratified into two groups: induced chemotherapy group (IC) of 10 patients and non-induced chemotherapy group (nIC) of 28 patients. The microbiota from oropharyngeal membrane was analyzed through 16S rRNA sequencing. Results Alpha-diversity (Shannon and Ace indexes) and weighted UniFrac based beta-diversity severely decreased in the HPSCC group when compared with CG. In pre-operative comparisons, PCoA and NMDS analyses showed microbial structures in the IC group were more similar to CG than nIC. Both IC group and nIC group yielded significantly diverse post-operative communities in contrast to their pre-operative counterparts, evident by the decrease in genera Veillonella and Fusobacterium and increase in genera Streptococcus and Gemella. Given that post-operative oropharynx microbiota showed no difference between IC and nIC groups, the IC group showed less accumulation in anaerobic communities. The abundance of genera Fusobacterium, Parvimonas, Actinomyces were enhanced in the advanced stages (III/IV). Conclusions Oropharynx microbiota in the HPSCC group presents dysbiosis with low diversity and abundance. Induced chemotherapy is beneficial in adjusting the oropharynx microbial environment leading to fewer amounts of anaerobe accumulation after operation. Higher amounts of Fusobacterium in advanced stages (III/IV) may influence the progression of HPSCC.
format	article
author	Hui-Ching Lau Chi-Yao Hsueh Hongli Gong Ji Sun Hui-Ying Huang Ming Zhang Liang Zhou
author_facet	Hui-Ching Lau Chi-Yao Hsueh Hongli Gong Ji Sun Hui-Ying Huang Ming Zhang Liang Zhou
author_sort	Hui-Ching Lau
title	Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
title_short	Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
title_full	Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
title_fullStr	Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
title_full_unstemmed	Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
title_sort	oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
publisher	BMC
publishDate	2021
url	https://doaj.org/article/57decd9bd49142948bc867eb34371d50
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Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery

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