Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa

ABSTRACT Coronavirus disease 2019 (COVID-19) has wreaked havoc across the globe; although the number of cases in Africa remains lower than in other regions, it is on a gradual upward trajectory. To date, COVID-19 cases have been reported in 54 out of 55 African countries. However, due to limited sev...

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Autores principales:	Tinashe K. Nyazika, Rabelani Kaela, Mathias Mugoni, Kudakwashe Musomekwa, Eric Kyei-Baafour, Simbarashe Chiwanda, Prichard T. Mapondera, Tatenda S. Makawa, Elliot M. Sithole, George Mavunganidze, Justen Manasa, Kondwani C. Jambo, Cuthbert Musarurwa
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	antibody rapid diagnostic test sample pooling rRT-PCR COVID-19 Africa Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/57e592d6e77845ae91428a72e1600e29
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spelling	oai:doaj.org-article:57e592d6e77845ae91428a72e1600e292021-11-15T15:30:52ZImplementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa10.1128/mSphere.00524-202379-5042https://doaj.org/article/57e592d6e77845ae91428a72e1600e292020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00524-20https://doaj.org/toc/2379-5042ABSTRACT Coronavirus disease 2019 (COVID-19) has wreaked havoc across the globe; although the number of cases in Africa remains lower than in other regions, it is on a gradual upward trajectory. To date, COVID-19 cases have been reported in 54 out of 55 African countries. However, due to limited severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time reverse transcription-PCR (rRT-PCR) testing capacity and scarcity of testing reagents, it is probable that the total number of cases could far exceed published statistics. In this viewpoint, using Ghana, Malawi, South Africa, and Zimbabwe as examples of countries that have implemented different testing strategies, we argue that the implementation of sample pooling for rRT-PCR over antibody rapid diagnostic testing could have a greater impact in assessing disease burden. Sample pooling offers huge advantages compared to single test rRT-PCR, as it reduces diagnostic costs, personnel time, burnout, and analytical run times. Africa is already strained in terms of testing resources for COVID-19; hence, cheaper alternative ways need to be implemented to conserve resources, maximize mass testing, and reduce transmission in the wider population.Tinashe K. NyazikaRabelani KaelaMathias MugoniKudakwashe MusomekwaEric Kyei-BaafourSimbarashe ChiwandaPrichard T. MaponderaTatenda S. MakawaElliot M. SitholeGeorge MavunganidzeJusten ManasaKondwani C. JamboCuthbert MusarurwaAmerican Society for Microbiologyarticleantibody rapid diagnostic testsample poolingrRT-PCRCOVID-19AfricaMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibody rapid diagnostic test sample pooling rRT-PCR COVID-19 Africa Microbiology QR1-502
spellingShingle	antibody rapid diagnostic test sample pooling rRT-PCR COVID-19 Africa Microbiology QR1-502 Tinashe K. Nyazika Rabelani Kaela Mathias Mugoni Kudakwashe Musomekwa Eric Kyei-Baafour Simbarashe Chiwanda Prichard T. Mapondera Tatenda S. Makawa Elliot M. Sithole George Mavunganidze Justen Manasa Kondwani C. Jambo Cuthbert Musarurwa Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa
description	ABSTRACT Coronavirus disease 2019 (COVID-19) has wreaked havoc across the globe; although the number of cases in Africa remains lower than in other regions, it is on a gradual upward trajectory. To date, COVID-19 cases have been reported in 54 out of 55 African countries. However, due to limited severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time reverse transcription-PCR (rRT-PCR) testing capacity and scarcity of testing reagents, it is probable that the total number of cases could far exceed published statistics. In this viewpoint, using Ghana, Malawi, South Africa, and Zimbabwe as examples of countries that have implemented different testing strategies, we argue that the implementation of sample pooling for rRT-PCR over antibody rapid diagnostic testing could have a greater impact in assessing disease burden. Sample pooling offers huge advantages compared to single test rRT-PCR, as it reduces diagnostic costs, personnel time, burnout, and analytical run times. Africa is already strained in terms of testing resources for COVID-19; hence, cheaper alternative ways need to be implemented to conserve resources, maximize mass testing, and reduce transmission in the wider population.
format	article
author	Tinashe K. Nyazika Rabelani Kaela Mathias Mugoni Kudakwashe Musomekwa Eric Kyei-Baafour Simbarashe Chiwanda Prichard T. Mapondera Tatenda S. Makawa Elliot M. Sithole George Mavunganidze Justen Manasa Kondwani C. Jambo Cuthbert Musarurwa
author_facet	Tinashe K. Nyazika Rabelani Kaela Mathias Mugoni Kudakwashe Musomekwa Eric Kyei-Baafour Simbarashe Chiwanda Prichard T. Mapondera Tatenda S. Makawa Elliot M. Sithole George Mavunganidze Justen Manasa Kondwani C. Jambo Cuthbert Musarurwa
author_sort	Tinashe K. Nyazika
title	Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa
title_short	Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa
title_full	Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa
title_fullStr	Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa
title_full_unstemmed	Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa
title_sort	implementation of antibody rapid diagnostic testing versus real-time reverse transcription-pcr sample pooling in the screening of covid-19: a case of different testing strategies in africa
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/57e592d6e77845ae91428a72e1600e29
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Implementation of Antibody Rapid Diagnostic Testing versus Real-Time Reverse Transcription-PCR Sample Pooling in the Screening of COVID-19: a Case of Different Testing Strategies in Africa

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