A rapid screening method to select microdialysis carriers for hydrophobic compounds.

Microdialysis is a minimally invasive sampling technique which is widely applied in many fields including clinical studies. This technique usually has limitation on sampling hydrophobic compounds as aqueous solutions are commonly used as the perfusates. The relative recovery of hydrophobic compounds...

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Autores principales:	Sin-Jie Wang, Hsiao-Ting Lu, Yu-Chao Wang, Hsin-Ying Huang, Chung-Shi Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57ef938c8d0e412ebf674cee464c2d22
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spelling	oai:doaj.org-article:57ef938c8d0e412ebf674cee464c2d222021-12-02T20:08:39ZA rapid screening method to select microdialysis carriers for hydrophobic compounds.1932-620310.1371/journal.pone.0256920https://doaj.org/article/57ef938c8d0e412ebf674cee464c2d222021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256920https://doaj.org/toc/1932-6203Microdialysis is a minimally invasive sampling technique which is widely applied in many fields including clinical studies. This technique usually has limitation on sampling hydrophobic compounds as aqueous solutions are commonly used as the perfusates. The relative recovery of hydrophobic compounds is often low and irreproducible because of the non-specific binding to microdialysis membranes or catheter tubing. Carriers such as cyclodextrins have been used to improve the recovery and consistency, however the identification of an optimal carrier can only be achieved after time-consuming and costly microdialysis experiments. We therefore developed a rapid, convenient, and low-cost method to identify the optimal carriers for sampling hydrophobic compounds with the use of centrifugal ultrafiltration. Doxorubicin was used as the model compound and its relative recoveries obtained from centrifugal ultrafiltration and from microdialysis were compared. The results show that the relative recoveries are highly correlated (correlation coefficient ≥ 0.9) between centrifugal ultrafiltration and microdialysis when different types or different concentrations of cyclodextrins were used as the carriers. In addition to doxorubicin, this method was further confirmed on three other drugs with different hydrophobicity. This method may facilitate and broaden the use of microdialysis perfusion on sampling or delivering hydrophobic substances in various applications.Sin-Jie WangHsiao-Ting LuYu-Chao WangHsin-Ying HuangChung-Shi YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256920 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sin-Jie Wang Hsiao-Ting Lu Yu-Chao Wang Hsin-Ying Huang Chung-Shi Yang A rapid screening method to select microdialysis carriers for hydrophobic compounds.
description	Microdialysis is a minimally invasive sampling technique which is widely applied in many fields including clinical studies. This technique usually has limitation on sampling hydrophobic compounds as aqueous solutions are commonly used as the perfusates. The relative recovery of hydrophobic compounds is often low and irreproducible because of the non-specific binding to microdialysis membranes or catheter tubing. Carriers such as cyclodextrins have been used to improve the recovery and consistency, however the identification of an optimal carrier can only be achieved after time-consuming and costly microdialysis experiments. We therefore developed a rapid, convenient, and low-cost method to identify the optimal carriers for sampling hydrophobic compounds with the use of centrifugal ultrafiltration. Doxorubicin was used as the model compound and its relative recoveries obtained from centrifugal ultrafiltration and from microdialysis were compared. The results show that the relative recoveries are highly correlated (correlation coefficient ≥ 0.9) between centrifugal ultrafiltration and microdialysis when different types or different concentrations of cyclodextrins were used as the carriers. In addition to doxorubicin, this method was further confirmed on three other drugs with different hydrophobicity. This method may facilitate and broaden the use of microdialysis perfusion on sampling or delivering hydrophobic substances in various applications.
format	article
author	Sin-Jie Wang Hsiao-Ting Lu Yu-Chao Wang Hsin-Ying Huang Chung-Shi Yang
author_facet	Sin-Jie Wang Hsiao-Ting Lu Yu-Chao Wang Hsin-Ying Huang Chung-Shi Yang
author_sort	Sin-Jie Wang
title	A rapid screening method to select microdialysis carriers for hydrophobic compounds.
title_short	A rapid screening method to select microdialysis carriers for hydrophobic compounds.
title_full	A rapid screening method to select microdialysis carriers for hydrophobic compounds.
title_fullStr	A rapid screening method to select microdialysis carriers for hydrophobic compounds.
title_full_unstemmed	A rapid screening method to select microdialysis carriers for hydrophobic compounds.
title_sort	rapid screening method to select microdialysis carriers for hydrophobic compounds.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/57ef938c8d0e412ebf674cee464c2d22
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A rapid screening method to select microdialysis carriers for hydrophobic compounds.

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