Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II

Cinnamic acid (CA), one of the main autotoxins secreted by cucumber roots during continuous cropping, inhibits plant growth and reduces yield. Silicon (Si) is an environmentally friendly element that alleviates abiotic stresses in plants, but the mechanism underlying its resistance to autotoxicity r...

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Autores principales:	Xin Meng, Shilei Luo, Mohammed Mujitaba Dawuda, Xueqin Gao, Shuya Wang, Jianming Xie, Zhongqi Tang, Zeci Liu, Yue Wu, Li Jin, Jian Lyu, Jihua Yu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Silicon Cinnamic acid Ascorbate-glutathione cycle Cucumber Antioxidant defense Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/57f1ed8dd96b4b4d95186b987fae727a
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spelling	oai:doaj.org-article:57f1ed8dd96b4b4d95186b987fae727a2021-11-06T04:14:21ZExogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II0147-651310.1016/j.ecoenv.2021.112879https://doaj.org/article/57f1ed8dd96b4b4d95186b987fae727a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S014765132100991Xhttps://doaj.org/toc/0147-6513Cinnamic acid (CA), one of the main autotoxins secreted by cucumber roots during continuous cropping, inhibits plant growth and reduces yield. Silicon (Si) is an environmentally friendly element that alleviates abiotic stresses in plants, but the mechanism underlying its resistance to autotoxicity remain unclear. Here, we used 0.8 mmol L−1 CA to study the effects of Si application on the growth, chlorophyll fluorescence, and ascorbate-glutathione (AsA-GSH) cycle of cucumber seedlings under CA inducing conditions. Our results indicated that CA significantly induced photoinhibition and overaccumulation of reactive oxygen species (ROS), thereby inhibiting cucumber growth. Treatment with 1.0 mmol L−1 Si improved plant height, stem diameter and biomass accumulation, and protected the photosynthetic electron transport function of photosystem II in the presence of CA. Similarly, Si application maintained the ROS status by increasing ascorbate (AsA) and glutathione (GSH) production, as well as the ratios of AsA/DHA and GSH/GSSG in both leaves and roots during CA stress. In addition, Si application in CA-treated seedlings enhanced the activity of key enzymes such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), and the transcription of several enzyme genes (CsAPX, CsMDHAR and CsGR） from the AsA-GSH cycle. These results suggest that exogenous Si enhances CA tolerance in cucumber seedlings by protecting photosystem II activity, upregulating AsA-GSH pathway, and reducing ROS levels.Xin MengShilei LuoMohammed Mujitaba DawudaXueqin GaoShuya WangJianming XieZhongqi TangZeci LiuYue WuLi JinJian LyuJihua YuElsevierarticleSiliconCinnamic acidAscorbate-glutathione cycleCucumberAntioxidant defenseEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112879- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silicon Cinnamic acid Ascorbate-glutathione cycle Cucumber Antioxidant defense Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Silicon Cinnamic acid Ascorbate-glutathione cycle Cucumber Antioxidant defense Environmental pollution TD172-193.5 Environmental sciences GE1-350 Xin Meng Shilei Luo Mohammed Mujitaba Dawuda Xueqin Gao Shuya Wang Jianming Xie Zhongqi Tang Zeci Liu Yue Wu Li Jin Jian Lyu Jihua Yu Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II
description	Cinnamic acid (CA), one of the main autotoxins secreted by cucumber roots during continuous cropping, inhibits plant growth and reduces yield. Silicon (Si) is an environmentally friendly element that alleviates abiotic stresses in plants, but the mechanism underlying its resistance to autotoxicity remain unclear. Here, we used 0.8 mmol L−1 CA to study the effects of Si application on the growth, chlorophyll fluorescence, and ascorbate-glutathione (AsA-GSH) cycle of cucumber seedlings under CA inducing conditions. Our results indicated that CA significantly induced photoinhibition and overaccumulation of reactive oxygen species (ROS), thereby inhibiting cucumber growth. Treatment with 1.0 mmol L−1 Si improved plant height, stem diameter and biomass accumulation, and protected the photosynthetic electron transport function of photosystem II in the presence of CA. Similarly, Si application maintained the ROS status by increasing ascorbate (AsA) and glutathione (GSH) production, as well as the ratios of AsA/DHA and GSH/GSSG in both leaves and roots during CA stress. In addition, Si application in CA-treated seedlings enhanced the activity of key enzymes such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), and the transcription of several enzyme genes (CsAPX, CsMDHAR and CsGR） from the AsA-GSH cycle. These results suggest that exogenous Si enhances CA tolerance in cucumber seedlings by protecting photosystem II activity, upregulating AsA-GSH pathway, and reducing ROS levels.
format	article
author	Xin Meng Shilei Luo Mohammed Mujitaba Dawuda Xueqin Gao Shuya Wang Jianming Xie Zhongqi Tang Zeci Liu Yue Wu Li Jin Jian Lyu Jihua Yu
author_facet	Xin Meng Shilei Luo Mohammed Mujitaba Dawuda Xueqin Gao Shuya Wang Jianming Xie Zhongqi Tang Zeci Liu Yue Wu Li Jin Jian Lyu Jihua Yu
author_sort	Xin Meng
title	Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II
title_short	Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II
title_full	Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II
title_fullStr	Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II
title_full_unstemmed	Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II
title_sort	exogenous silicon enhances the systemic defense of cucumber leaves and roots against ca-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem ii
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/57f1ed8dd96b4b4d95186b987fae727a
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Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II

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