A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection

The nucleic acid-based technique has been widely utilized in many fields including for on-site detection. However, traditional molecular detection techniques encounter limitations like relying on instruments, time consuming or complex operation, and cannot meet the demands of on-site testing. In thi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiao Fu Wang, Wen Qiang Chen, Jian Li Guo, Cheng Peng, Xiao Yun Chen, Xiao Li Xu, Wei Wei, Lei Yang, Jian Ca, Jun Feng Xu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	rapid DNA extraction chemical heating RAA on-site detection nucleic acid Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/57f1ef78809e4b39b09308f8106d7ec5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:57f1ef78809e4b39b09308f8106d7ec5
record_format	dspace
spelling	oai:doaj.org-article:57f1ef78809e4b39b09308f8106d7ec52021-11-22T05:23:42ZA Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection2296-418510.3389/fbioe.2021.764306https://doaj.org/article/57f1ef78809e4b39b09308f8106d7ec52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.764306/fullhttps://doaj.org/toc/2296-4185The nucleic acid-based technique has been widely utilized in many fields including for on-site detection. However, traditional molecular detection techniques encounter limitations like relying on instruments, time consuming or complex operation, and cannot meet the demands of on-site testing. In this study, a rapid DNA extraction method (RDEM), recombinase aided amplification (RAA), and chemical heating packet (CHP) are integrated and termed as RRC platform for on-site detection of nucleic acid. For demonstration purposes, SHZD32-1 (a new transgenic soybean line from China) was detected using the novel platform to demonstrate its feasibility and capability for on-site detection. Using the RDEM, high-quality DNA appropriate for molecular detection was quickly extracted in 3–5 min. The heat energy generated by CHP was met the temperature requirements of RAA. Using the RRC platform, the whole detection process can be accomplished within only 30 min, and the results can be visually detected with glasses under blue light. No special or expensive instrument was needed for the detection process. This study provides a novel approach for on-site detection of nucleic acids besides providing valuable insight on related future research.Xiao Fu WangXiao Fu WangWen Qiang ChenWen Qiang ChenJian Li GuoCheng PengCheng PengXiao Yun ChenXiao Yun ChenXiao Li XuXiao Li XuWei WeiLei YangJian CaJun Feng XuJun Feng XuFrontiers Media S.A.articlerapid DNA extractionchemical heatingRAAon-site detectionnucleic acidBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rapid DNA extraction chemical heating RAA on-site detection nucleic acid Biotechnology TP248.13-248.65
spellingShingle	rapid DNA extraction chemical heating RAA on-site detection nucleic acid Biotechnology TP248.13-248.65 Xiao Fu Wang Xiao Fu Wang Wen Qiang Chen Wen Qiang Chen Jian Li Guo Cheng Peng Cheng Peng Xiao Yun Chen Xiao Yun Chen Xiao Li Xu Xiao Li Xu Wei Wei Lei Yang Jian Ca Jun Feng Xu Jun Feng Xu A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection
description	The nucleic acid-based technique has been widely utilized in many fields including for on-site detection. However, traditional molecular detection techniques encounter limitations like relying on instruments, time consuming or complex operation, and cannot meet the demands of on-site testing. In this study, a rapid DNA extraction method (RDEM), recombinase aided amplification (RAA), and chemical heating packet (CHP) are integrated and termed as RRC platform for on-site detection of nucleic acid. For demonstration purposes, SHZD32-1 (a new transgenic soybean line from China) was detected using the novel platform to demonstrate its feasibility and capability for on-site detection. Using the RDEM, high-quality DNA appropriate for molecular detection was quickly extracted in 3–5 min. The heat energy generated by CHP was met the temperature requirements of RAA. Using the RRC platform, the whole detection process can be accomplished within only 30 min, and the results can be visually detected with glasses under blue light. No special or expensive instrument was needed for the detection process. This study provides a novel approach for on-site detection of nucleic acids besides providing valuable insight on related future research.
format	article
author	Xiao Fu Wang Xiao Fu Wang Wen Qiang Chen Wen Qiang Chen Jian Li Guo Cheng Peng Cheng Peng Xiao Yun Chen Xiao Yun Chen Xiao Li Xu Xiao Li Xu Wei Wei Lei Yang Jian Ca Jun Feng Xu Jun Feng Xu
author_facet	Xiao Fu Wang Xiao Fu Wang Wen Qiang Chen Wen Qiang Chen Jian Li Guo Cheng Peng Cheng Peng Xiao Yun Chen Xiao Yun Chen Xiao Li Xu Xiao Li Xu Wei Wei Lei Yang Jian Ca Jun Feng Xu Jun Feng Xu
author_sort	Xiao Fu Wang
title	A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection
title_short	A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection
title_full	A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection
title_fullStr	A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection
title_full_unstemmed	A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection
title_sort	fast, visual, and instrument-free platform involving rapid dna extraction, chemical heating, and recombinase aided amplification for on-site nucleic acid detection
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/57f1ef78809e4b39b09308f8106d7ec5
work_keys_str_mv	AT xiaofuwang afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaofuwang afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT wenqiangchen afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT wenqiangchen afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT jianliguo afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT chengpeng afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT chengpeng afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaoyunchen afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaoyunchen afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaolixu afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaolixu afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT weiwei afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT leiyang afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT jianca afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT junfengxu afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT junfengxu afastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaofuwang fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaofuwang fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT wenqiangchen fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT wenqiangchen fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT jianliguo fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT chengpeng fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT chengpeng fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaoyunchen fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaoyunchen fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaolixu fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT xiaolixu fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT weiwei fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT leiyang fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT jianca fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT junfengxu fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection AT junfengxu fastvisualandinstrumentfreeplatforminvolvingrapiddnaextractionchemicalheatingandrecombinaseaidedamplificationforonsitenucleicaciddetection
_version_	1718418103454400512

A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection

Ejemplares similares