Impact of acute metal stress in Saccharomyces cerevisiae.

Although considered as essential cofactors for a variety of enzymatic reactions and for important structural and functional roles in cell metabolism, metals at high concentrations are potent toxic pollutants and pose complex biochemical problems for cells. We report results of single dose acute toxi...

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Autores principales:	Dagmar Hosiner, Susanne Gerber, Hella Lichtenberg-Fraté, Walter Glaser, Christoph Schüller, Edda Klipp
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57f2b3b854a14c6b8c7c82d79e682deb
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spelling	oai:doaj.org-article:57f2b3b854a14c6b8c7c82d79e682deb2021-11-18T08:38:16ZImpact of acute metal stress in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0083330https://doaj.org/article/57f2b3b854a14c6b8c7c82d79e682deb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416162/?tool=EBIhttps://doaj.org/toc/1932-6203Although considered as essential cofactors for a variety of enzymatic reactions and for important structural and functional roles in cell metabolism, metals at high concentrations are potent toxic pollutants and pose complex biochemical problems for cells. We report results of single dose acute toxicity testing in the model organism S. cerevisiae. The effects of moderate toxic concentrations of 10 different human health relevant metals, Ag(+), Al(3+), As(3+), Cd(2+), Co(2+), Hg(2+), Mn(2+), Ni(2+), V(3+), and Zn(2+), following short-term exposure were analyzed by transcription profiling to provide the identification of early-on target genes or pathways. In contrast to common acute toxicity tests where defined endpoints are monitored we focused on the entire genomic response. We provide evidence that the induction of central elements of the oxidative stress response by the majority of investigated metals is the basic detoxification process against short-term metal exposure. General detoxification mechanisms also comprised the induction of genes coding for chaperones and those for chelation of metal ions via siderophores and amino acids. Hierarchical clustering, transcription factor analyses, and gene ontology data further revealed activation of genes involved in metal-specific protein catabolism along with repression of growth-related processes such as protein synthesis. Metal ion group specific differences in the expression responses with shared transcriptional regulators for both, up-regulation and repression were also observed. Additionally, some processes unique for individual metals were evident as well. In view of current concerns regarding environmental pollution our results may support ongoing attempts to develop methods to monitor potentially hazardous areas or liquids and to establish standardized tests using suitable eukaryotic a model organism.Dagmar HosinerSusanne GerberHella Lichtenberg-FratéWalter GlaserChristoph SchüllerEdda KlippPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e83330 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dagmar Hosiner Susanne Gerber Hella Lichtenberg-Fraté Walter Glaser Christoph Schüller Edda Klipp Impact of acute metal stress in Saccharomyces cerevisiae.
description	Although considered as essential cofactors for a variety of enzymatic reactions and for important structural and functional roles in cell metabolism, metals at high concentrations are potent toxic pollutants and pose complex biochemical problems for cells. We report results of single dose acute toxicity testing in the model organism S. cerevisiae. The effects of moderate toxic concentrations of 10 different human health relevant metals, Ag(+), Al(3+), As(3+), Cd(2+), Co(2+), Hg(2+), Mn(2+), Ni(2+), V(3+), and Zn(2+), following short-term exposure were analyzed by transcription profiling to provide the identification of early-on target genes or pathways. In contrast to common acute toxicity tests where defined endpoints are monitored we focused on the entire genomic response. We provide evidence that the induction of central elements of the oxidative stress response by the majority of investigated metals is the basic detoxification process against short-term metal exposure. General detoxification mechanisms also comprised the induction of genes coding for chaperones and those for chelation of metal ions via siderophores and amino acids. Hierarchical clustering, transcription factor analyses, and gene ontology data further revealed activation of genes involved in metal-specific protein catabolism along with repression of growth-related processes such as protein synthesis. Metal ion group specific differences in the expression responses with shared transcriptional regulators for both, up-regulation and repression were also observed. Additionally, some processes unique for individual metals were evident as well. In view of current concerns regarding environmental pollution our results may support ongoing attempts to develop methods to monitor potentially hazardous areas or liquids and to establish standardized tests using suitable eukaryotic a model organism.
format	article
author	Dagmar Hosiner Susanne Gerber Hella Lichtenberg-Fraté Walter Glaser Christoph Schüller Edda Klipp
author_facet	Dagmar Hosiner Susanne Gerber Hella Lichtenberg-Fraté Walter Glaser Christoph Schüller Edda Klipp
author_sort	Dagmar Hosiner
title	Impact of acute metal stress in Saccharomyces cerevisiae.
title_short	Impact of acute metal stress in Saccharomyces cerevisiae.
title_full	Impact of acute metal stress in Saccharomyces cerevisiae.
title_fullStr	Impact of acute metal stress in Saccharomyces cerevisiae.
title_full_unstemmed	Impact of acute metal stress in Saccharomyces cerevisiae.
title_sort	impact of acute metal stress in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/57f2b3b854a14c6b8c7c82d79e682deb
work_keys_str_mv	AT dagmarhosiner impactofacutemetalstressinsaccharomycescerevisiae AT susannegerber impactofacutemetalstressinsaccharomycescerevisiae AT hellalichtenbergfrate impactofacutemetalstressinsaccharomycescerevisiae AT walterglaser impactofacutemetalstressinsaccharomycescerevisiae AT christophschuller impactofacutemetalstressinsaccharomycescerevisiae AT eddaklipp impactofacutemetalstressinsaccharomycescerevisiae
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Impact of acute metal stress in Saccharomyces cerevisiae.

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