Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury

Abstract Age-related macular degeneration (AMD), a leading contributor of vision loss, currently lacks comprehensive treatment. While AMD histopathology involves retinal pigment epithelium (RPE) injury associated with immune cell infiltration, the nature of immune cell responses to RPE injury remain...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wenxin Ma, Yikui Zhang, Chun Gao, Robert N. Fariss, Johnny Tam, Wai T. Wong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57f2e24443184b2d82ab1f09abe0f5d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:57f2e24443184b2d82ab1f09abe0f5d3
record_format	dspace
spelling	oai:doaj.org-article:57f2e24443184b2d82ab1f09abe0f5d32021-12-02T12:32:42ZMonocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury10.1038/s41598-017-08702-72045-2322https://doaj.org/article/57f2e24443184b2d82ab1f09abe0f5d32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08702-7https://doaj.org/toc/2045-2322Abstract Age-related macular degeneration (AMD), a leading contributor of vision loss, currently lacks comprehensive treatment. While AMD histopathology involves retinal pigment epithelium (RPE) injury associated with immune cell infiltration, the nature of immune cell responses to RPE injury remains undefined. We induced RPE injury pharmacologically and genetically in transgenic mouse models in which microglia and systemic monocytes were separately tagged, enabling a spatial and temporal dissection of the relative contributions of microglia vs. monocytes to post-injury changes. We found that myeloid cell responses to RPE injury occur in stages: (1) an early mobilization of endogenous microglia from the inner retina to the RPE layer, followed by (2) subsequent monocyte infiltration from the retinal vasculature into the inner retina that replenishes the local myeloid cell population in a CCR2-regulated manner. These altered distributions of myeloid cells post-injury were long-lived, with recruited monocytes acquiring the distribution, markers, and morphologies of neighboring endogenous microglia in a durable manner. These findings indicate the role played by infiltrating monocytes in maintaining myeloid cell homeostasis in the retina following AMD-relevant RPE injury and provide a foundation for understanding and therapeutically modulating immune aspects in retinal disease.Wenxin MaYikui ZhangChun GaoRobert N. FarissJohnny TamWai T. WongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-18 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenxin Ma Yikui Zhang Chun Gao Robert N. Fariss Johnny Tam Wai T. Wong Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury
description	Abstract Age-related macular degeneration (AMD), a leading contributor of vision loss, currently lacks comprehensive treatment. While AMD histopathology involves retinal pigment epithelium (RPE) injury associated with immune cell infiltration, the nature of immune cell responses to RPE injury remains undefined. We induced RPE injury pharmacologically and genetically in transgenic mouse models in which microglia and systemic monocytes were separately tagged, enabling a spatial and temporal dissection of the relative contributions of microglia vs. monocytes to post-injury changes. We found that myeloid cell responses to RPE injury occur in stages: (1) an early mobilization of endogenous microglia from the inner retina to the RPE layer, followed by (2) subsequent monocyte infiltration from the retinal vasculature into the inner retina that replenishes the local myeloid cell population in a CCR2-regulated manner. These altered distributions of myeloid cells post-injury were long-lived, with recruited monocytes acquiring the distribution, markers, and morphologies of neighboring endogenous microglia in a durable manner. These findings indicate the role played by infiltrating monocytes in maintaining myeloid cell homeostasis in the retina following AMD-relevant RPE injury and provide a foundation for understanding and therapeutically modulating immune aspects in retinal disease.
format	article
author	Wenxin Ma Yikui Zhang Chun Gao Robert N. Fariss Johnny Tam Wai T. Wong
author_facet	Wenxin Ma Yikui Zhang Chun Gao Robert N. Fariss Johnny Tam Wai T. Wong
author_sort	Wenxin Ma
title	Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury
title_short	Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury
title_full	Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury
title_fullStr	Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury
title_full_unstemmed	Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury
title_sort	monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/57f2e24443184b2d82ab1f09abe0f5d3
work_keys_str_mv	AT wenxinma monocyteinfiltrationandproliferationreestablishmyeloidcellhomeostasisinthemouseretinafollowingretinalpigmentepithelialcellinjury AT yikuizhang monocyteinfiltrationandproliferationreestablishmyeloidcellhomeostasisinthemouseretinafollowingretinalpigmentepithelialcellinjury AT chungao monocyteinfiltrationandproliferationreestablishmyeloidcellhomeostasisinthemouseretinafollowingretinalpigmentepithelialcellinjury AT robertnfariss monocyteinfiltrationandproliferationreestablishmyeloidcellhomeostasisinthemouseretinafollowingretinalpigmentepithelialcellinjury AT johnnytam monocyteinfiltrationandproliferationreestablishmyeloidcellhomeostasisinthemouseretinafollowingretinalpigmentepithelialcellinjury AT waitwong monocyteinfiltrationandproliferationreestablishmyeloidcellhomeostasisinthemouseretinafollowingretinalpigmentepithelialcellinjury
_version_	1718393978985906176

Monocyte infiltration and proliferation reestablish myeloid cell homeostasis in the mouse retina following retinal pigment epithelial cell injury

Ejemplares similares