Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell
Objective: To synthesize and characterize MCM-41-IL-4 and evaluates its immunomodulatory effect on Raw 264.7 cells. Methods: we synthesized and characterized MCM-41 type mesoporous silica nanoparticles (MSNs) and loaded it with interleukin 4 (IL-4); CCK-8 and live and dead staining were used to e...
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Editorial Board of Journal of Hainan Medical University
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/57f62bef28ae405fb017b463fbda8fe4 |
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| Sumario: | Objective: To synthesize and characterize MCM-41-IL-4 and evaluates its immunomodulatory
effect on Raw 264.7 cells. Methods: we synthesized and characterized MCM-41 type
mesoporous silica nanoparticles (MSNs) and loaded it with interleukin 4 (IL-4); CCK-8 and
live and dead staining were used to evaluate Raw 264.7 cell viability; Immunofluorescence was
used to study morphology of Raw 264.7 cells; Quantitative real-time polymerase chain reaction
（qRT-PCR）were used to study the gene expression of inflammatory factors of Raw 264.7
cells; After the stimulation of human umbilical vein endothelial cells (HUVECs) with MCM-
41/RAW 264.7 and MCM-41-IL-4/ Raw 264.7 condition medium, the gene expression of
angiogenic factor matrix metallopeptidase 9 (MMP9) and kinase insert domain protein receptor
(VEGF) pathway-related factors from HUVECs was assessed by qRT-PCR. Results: MCM-
41-IL-4 can stimulate the proliferation of RAW 264.7 cells; MCM-41-IL-4 can induce RAW
264.7 cells express more the F-actin; MCM-41-IL-4 can increase the expression of M2-related
genes transforming growth factor, beta 1（Tgfb1）, interleukin 1 receptor antagonist（IL-
1ra）and arginase, liver (Arg-1) and decrease the expression of M1-related gene interleukin 6
(IL-6); Increased gene expression of MMP9 and platelet derived growth factor receptor alpha
(PDGFRα) of HUVECs of MCM-41/Raw 264.7 condition medium was observed. Conclusion:
MCM-41-IL-4 can stimulate the proliferation of RAW 264.7 cells, increase the expression of
M2-related genes, decrease the expression of M1-related gene and induce the transformation
of M2 macrophage which may be used as an immunomodulatory agent for bone tissue
engineering applications. |
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