Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell

Objective: To synthesize and characterize MCM-41-IL-4 and evaluates its immunomodulatory effect on Raw 264.7 cells. Methods: we synthesized and characterized MCM-41 type mesoporous silica nanoparticles (MSNs) and loaded it with interleukin 4 (IL-4); CCK-8 and live and dead staining were used to e...

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Autor principal:	Pei-Xuan Peng
Formato:	article
Lenguaje:	EN
Publicado:	Editorial Board of Journal of Hainan Medical University 2021
Materias:	mesoporous silica nanoparticles il-4 macrophage human umbilical vein endothelial cells Medicine R
Acceso en línea:	https://doaj.org/article/57f62bef28ae405fb017b463fbda8fe4
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spelling	oai:doaj.org-article:57f62bef28ae405fb017b463fbda8fe42021-11-16T01:27:08ZImmunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell1007-1237https://doaj.org/article/57f62bef28ae405fb017b463fbda8fe42021-09-01T00:00:00Zhttp://www.hnykdxxb.com/PDF/202119/05.pdfhttps://doaj.org/toc/1007-1237Objective: To synthesize and characterize MCM-41-IL-4 and evaluates its immunomodulatory effect on Raw 264.7 cells. Methods: we synthesized and characterized MCM-41 type mesoporous silica nanoparticles (MSNs) and loaded it with interleukin 4 (IL-4); CCK-8 and live and dead staining were used to evaluate Raw 264.7 cell viability; Immunofluorescence was used to study morphology of Raw 264.7 cells; Quantitative real-time polymerase chain reaction （qRT-PCR）were used to study the gene expression of inflammatory factors of Raw 264.7 cells; After the stimulation of human umbilical vein endothelial cells (HUVECs) with MCM- 41/RAW 264.7 and MCM-41-IL-4/ Raw 264.7 condition medium, the gene expression of angiogenic factor matrix metallopeptidase 9 (MMP9) and kinase insert domain protein receptor (VEGF) pathway-related factors from HUVECs was assessed by qRT-PCR. Results: MCM- 41-IL-4 can stimulate the proliferation of RAW 264.7 cells; MCM-41-IL-4 can induce RAW 264.7 cells express more the F-actin; MCM-41-IL-4 can increase the expression of M2-related genes transforming growth factor, beta 1（Tgfb1）, interleukin 1 receptor antagonist（IL- 1ra）and arginase, liver (Arg-1) and decrease the expression of M1-related gene interleukin 6 (IL-6); Increased gene expression of MMP9 and platelet derived growth factor receptor alpha (PDGFRα) of HUVECs of MCM-41/Raw 264.7 condition medium was observed. Conclusion: MCM-41-IL-4 can stimulate the proliferation of RAW 264.7 cells, increase the expression of M2-related genes, decrease the expression of M1-related gene and induce the transformation of M2 macrophage which may be used as an immunomodulatory agent for bone tissue engineering applications.Pei-Xuan PengEditorial Board of Journal of Hainan Medical Universityarticlemesoporous silica nanoparticlesil-4macrophagehuman umbilical vein endothelial cellsMedicineRENJournal of Hainan Medical University, Vol 27, Iss 19, Pp 21-26 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesoporous silica nanoparticles il-4 macrophage human umbilical vein endothelial cells Medicine R
spellingShingle	mesoporous silica nanoparticles il-4 macrophage human umbilical vein endothelial cells Medicine R Pei-Xuan Peng Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell
description	Objective: To synthesize and characterize MCM-41-IL-4 and evaluates its immunomodulatory effect on Raw 264.7 cells. Methods: we synthesized and characterized MCM-41 type mesoporous silica nanoparticles (MSNs) and loaded it with interleukin 4 (IL-4); CCK-8 and live and dead staining were used to evaluate Raw 264.7 cell viability; Immunofluorescence was used to study morphology of Raw 264.7 cells; Quantitative real-time polymerase chain reaction （qRT-PCR）were used to study the gene expression of inflammatory factors of Raw 264.7 cells; After the stimulation of human umbilical vein endothelial cells (HUVECs) with MCM- 41/RAW 264.7 and MCM-41-IL-4/ Raw 264.7 condition medium, the gene expression of angiogenic factor matrix metallopeptidase 9 (MMP9) and kinase insert domain protein receptor (VEGF) pathway-related factors from HUVECs was assessed by qRT-PCR. Results: MCM- 41-IL-4 can stimulate the proliferation of RAW 264.7 cells; MCM-41-IL-4 can induce RAW 264.7 cells express more the F-actin; MCM-41-IL-4 can increase the expression of M2-related genes transforming growth factor, beta 1（Tgfb1）, interleukin 1 receptor antagonist（IL- 1ra）and arginase, liver (Arg-1) and decrease the expression of M1-related gene interleukin 6 (IL-6); Increased gene expression of MMP9 and platelet derived growth factor receptor alpha (PDGFRα) of HUVECs of MCM-41/Raw 264.7 condition medium was observed. Conclusion: MCM-41-IL-4 can stimulate the proliferation of RAW 264.7 cells, increase the expression of M2-related genes, decrease the expression of M1-related gene and induce the transformation of M2 macrophage which may be used as an immunomodulatory agent for bone tissue engineering applications.
format	article
author	Pei-Xuan Peng
author_facet	Pei-Xuan Peng
author_sort	Pei-Xuan Peng
title	Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell
title_short	Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell
title_full	Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell
title_fullStr	Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell
title_full_unstemmed	Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell
title_sort	immunomodulatory effect of mcm-41-il-4 on raw 264.7 cell
publisher	Editorial Board of Journal of Hainan Medical University
publishDate	2021
url	https://doaj.org/article/57f62bef28ae405fb017b463fbda8fe4
work_keys_str_mv	AT peixuanpeng immunomodulatoryeffectofmcm41il4onraw2647cell
_version_	1718426787963207680

Immunomodulatory effect of MCM-41-IL-4 on Raw 264.7 cell

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