Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)

Abstract Glyphosate has been used for more than 15 years for weed management in citrus groves in the Gulf of Mexico, at up to 3–4 applications per year. Goosegrass (Eleusine indica (L.) Gaertn.) control has sometimes failed. In this research, the mechanisms governing three goosegrass biotypes (Ein-O...

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Autores principales:	Javid Gherekhloo, Pablo T. Fernández-Moreno, Ricardo Alcántara-de la Cruz, Eduardo Sánchez-González, Hugo E. Cruz-Hipolito, José A. Domínguez-Valenzuela, Rafael De Prado
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/57f6d4198577416da8b0939f566bb977
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spelling	oai:doaj.org-article:57f6d4198577416da8b0939f566bb9772021-12-02T12:32:12ZPro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)10.1038/s41598-017-06772-12045-2322https://doaj.org/article/57f6d4198577416da8b0939f566bb9772017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06772-1https://doaj.org/toc/2045-2322Abstract Glyphosate has been used for more than 15 years for weed management in citrus groves in the Gulf of Mexico, at up to 3–4 applications per year. Goosegrass (Eleusine indica (L.) Gaertn.) control has sometimes failed. In this research, the mechanisms governing three goosegrass biotypes (Ein-Or from an orange grove, and Ein-Pl1 and Ein-Pl2 from Persian lime groves) with suspected resistance to glyphosate were characterized and compared to a susceptible biotype (Ein-S). Dose-response and shikimate accumulation assays confirmed resistance of the resistant (R) biotypes. There were no differences in glyphosate absorption, but the R biotypes retained up to 62–78% of the herbicide in the treated leaf at 96 h after treatment (HAT), in comparison to the Ein-S biotype (36%). The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity in the Ein-Or and Ein-S biotypes was over 100-fold lower than the Ein-Pl1 and Ein-Pl2 ones. The latter showed a high EPSPS-basal activity, a mutation at Pro-106-Ser position in the EPSPS gene, and EPSPS overexpression. The EPSPS basal and EPSPS overexpression were positively correlated. The R goosegrass biotypes displayed poor glyphosate translocation. Furthermore, this grassweed showed, for the first time, two mechanisms at the target-site level (Pro-106-Ser mutation + EPSPS overexpression) acting together simultaneously against glyphosate.Javid GherekhlooPablo T. Fernández-MorenoRicardo Alcántara-de la CruzEduardo Sánchez-GonzálezHugo E. Cruz-HipolitoJosé A. Domínguez-ValenzuelaRafael De PradoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Javid Gherekhloo Pablo T. Fernández-Moreno Ricardo Alcántara-de la Cruz Eduardo Sánchez-González Hugo E. Cruz-Hipolito José A. Domínguez-Valenzuela Rafael De Prado Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)
description	Abstract Glyphosate has been used for more than 15 years for weed management in citrus groves in the Gulf of Mexico, at up to 3–4 applications per year. Goosegrass (Eleusine indica (L.) Gaertn.) control has sometimes failed. In this research, the mechanisms governing three goosegrass biotypes (Ein-Or from an orange grove, and Ein-Pl1 and Ein-Pl2 from Persian lime groves) with suspected resistance to glyphosate were characterized and compared to a susceptible biotype (Ein-S). Dose-response and shikimate accumulation assays confirmed resistance of the resistant (R) biotypes. There were no differences in glyphosate absorption, but the R biotypes retained up to 62–78% of the herbicide in the treated leaf at 96 h after treatment (HAT), in comparison to the Ein-S biotype (36%). The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity in the Ein-Or and Ein-S biotypes was over 100-fold lower than the Ein-Pl1 and Ein-Pl2 ones. The latter showed a high EPSPS-basal activity, a mutation at Pro-106-Ser position in the EPSPS gene, and EPSPS overexpression. The EPSPS basal and EPSPS overexpression were positively correlated. The R goosegrass biotypes displayed poor glyphosate translocation. Furthermore, this grassweed showed, for the first time, two mechanisms at the target-site level (Pro-106-Ser mutation + EPSPS overexpression) acting together simultaneously against glyphosate.
format	article
author	Javid Gherekhloo Pablo T. Fernández-Moreno Ricardo Alcántara-de la Cruz Eduardo Sánchez-González Hugo E. Cruz-Hipolito José A. Domínguez-Valenzuela Rafael De Prado
author_facet	Javid Gherekhloo Pablo T. Fernández-Moreno Ricardo Alcántara-de la Cruz Eduardo Sánchez-González Hugo E. Cruz-Hipolito José A. Domínguez-Valenzuela Rafael De Prado
author_sort	Javid Gherekhloo
title	Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)
title_short	Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)
title_full	Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)
title_fullStr	Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)
title_full_unstemmed	Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)
title_sort	pro-106-ser mutation and epsps overexpression acting together simultaneously in glyphosate-resistant goosegrass (eleusine indica)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/57f6d4198577416da8b0939f566bb977
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Pro-106-Ser mutation and EPSPS overexpression acting together simultaneously in glyphosate-resistant goosegrass (Eleusine indica)

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