Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice

Leake Gebremeskel,1 Kald Beshir Tuem,2 Tewolde Teklu1 1Pharmacology and Toxicology Unit, Department of Pharmacy, College of Health Sciences, Aksum University, Aksum, Ethiopia; 2Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Eth...

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Autores principales:	Gebremeskel L, Beshir Tuem K, Teklu T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	becium grandiflorum anti-diabetic antihyperglycemic streptozotocin Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/5800ebe32c9f4364a2d187387ee328a0
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spelling	oai:doaj.org-article:5800ebe32c9f4364a2d187387ee328a02021-12-02T09:53:47ZEvaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice1178-7007https://doaj.org/article/5800ebe32c9f4364a2d187387ee328a02020-05-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-antidiabetic-effect-of-ethanolic-leaves-extract-of-beciu-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Leake Gebremeskel,1 Kald Beshir Tuem,2 Tewolde Teklu1 1Pharmacology and Toxicology Unit, Department of Pharmacy, College of Health Sciences, Aksum University, Aksum, Ethiopia; 2Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaCorrespondence: Leake GebremeskelPharmacology and Toxicology Unit, Department of Pharmacy, College of Health Sciences, Aksum University, Postal 298, Aksum, EthiopiaTel +251 913726205Fax +251348750244Email lemeskel2005@gmail.comBackground: Becium grandiflorum has been used traditionally for treatment of different ailments including diabetes mellitus although it lacks scientific evidence. Thus, the present study was aimed at evaluating the antidiabetic effect of Becium grandiflorum in streptozotocin (STZ)-induced diabetic mice.Methods: The antidiabetic activity of hydro-ethanolic (30:70) leaf extract of Becium grandiflorum was evaluated in STZ (45 mg/kg)-induced diabetic and normal mice. Antihyperglycemic, hypoglycemic, oral glucose tolerance and body weight change effects of the extract were assessed after administering three doses of the extract (200, 400 and 600 mg/kg), glibenclamide 5 mg/kg (reference drug) and 2% Tween 80 (vehicle). One-way analysis of variance and Tukey’s post hoc test were used for data analysis.Results: All doses of the extract (200 mg/kg (p< 0.05), 400 mg/kg (p< 0.05) and 600 mg/kg (p< 0.01)) and glibenclamide 5 mg/kg (p< 0.001) showed statistically significant blood glucose level reduction in normal mice as compared to Tween 80. The hydroalcoholic extract at a dose of 200 mg/kg (p< 0.05), 400 mg/kg (p< 0.01) and 600 mg/kg (p< 0.001) showed better blood glucose tolerance after 60,  120 and 180-minute treatment duration in normal mice as compared to negative control. In diabetic mice, Becium grandiflorum doses and the reference drug caused maximum reduction in blood glucose level at the end of the  15th day of treatment by 17.61%, 22.52%, 24.62% and 34.12%, respectively. The extract’s doses and the standard drug showed significant (p< 0.05) improvement in body weight while the diabetic control continued to lose their body weight.Conclusion: Thus, Becium grandiflorum exhibits antihyperglycemic activity in STZ-induced diabetic mice, and shows improvement in oral glucose tolerance and body weight, which justifies the claimed use of the plant in ameliorating diabetes mellitus in Ethiopian folk medicine.Keywords: Becium grandiflorum, antidiabetic, antihyperglycemic, streptozotocinGebremeskel LBeshir Tuem KTeklu TDove Medical Pressarticlebecium grandiflorumanti-diabeticantihyperglycemicstreptozotocinSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 1481-1489 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	becium grandiflorum anti-diabetic antihyperglycemic streptozotocin Specialties of internal medicine RC581-951
spellingShingle	becium grandiflorum anti-diabetic antihyperglycemic streptozotocin Specialties of internal medicine RC581-951 Gebremeskel L Beshir Tuem K Teklu T Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice
description	Leake Gebremeskel,1 Kald Beshir Tuem,2 Tewolde Teklu1 1Pharmacology and Toxicology Unit, Department of Pharmacy, College of Health Sciences, Aksum University, Aksum, Ethiopia; 2Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaCorrespondence: Leake GebremeskelPharmacology and Toxicology Unit, Department of Pharmacy, College of Health Sciences, Aksum University, Postal 298, Aksum, EthiopiaTel +251 913726205Fax +251348750244Email lemeskel2005@gmail.comBackground: Becium grandiflorum has been used traditionally for treatment of different ailments including diabetes mellitus although it lacks scientific evidence. Thus, the present study was aimed at evaluating the antidiabetic effect of Becium grandiflorum in streptozotocin (STZ)-induced diabetic mice.Methods: The antidiabetic activity of hydro-ethanolic (30:70) leaf extract of Becium grandiflorum was evaluated in STZ (45 mg/kg)-induced diabetic and normal mice. Antihyperglycemic, hypoglycemic, oral glucose tolerance and body weight change effects of the extract were assessed after administering three doses of the extract (200, 400 and 600 mg/kg), glibenclamide 5 mg/kg (reference drug) and 2% Tween 80 (vehicle). One-way analysis of variance and Tukey’s post hoc test were used for data analysis.Results: All doses of the extract (200 mg/kg (p< 0.05), 400 mg/kg (p< 0.05) and 600 mg/kg (p< 0.01)) and glibenclamide 5 mg/kg (p< 0.001) showed statistically significant blood glucose level reduction in normal mice as compared to Tween 80. The hydroalcoholic extract at a dose of 200 mg/kg (p< 0.05), 400 mg/kg (p< 0.01) and 600 mg/kg (p< 0.001) showed better blood glucose tolerance after 60,  120 and 180-minute treatment duration in normal mice as compared to negative control. In diabetic mice, Becium grandiflorum doses and the reference drug caused maximum reduction in blood glucose level at the end of the  15th day of treatment by 17.61%, 22.52%, 24.62% and 34.12%, respectively. The extract’s doses and the standard drug showed significant (p< 0.05) improvement in body weight while the diabetic control continued to lose their body weight.Conclusion: Thus, Becium grandiflorum exhibits antihyperglycemic activity in STZ-induced diabetic mice, and shows improvement in oral glucose tolerance and body weight, which justifies the claimed use of the plant in ameliorating diabetes mellitus in Ethiopian folk medicine.Keywords: Becium grandiflorum, antidiabetic, antihyperglycemic, streptozotocin
format	article
author	Gebremeskel L Beshir Tuem K Teklu T
author_facet	Gebremeskel L Beshir Tuem K Teklu T
author_sort	Gebremeskel L
title	Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice
title_short	Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice
title_full	Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice
title_fullStr	Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice
title_full_unstemmed	Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice
title_sort	evaluation of antidiabetic effect of ethanolic leaves extract of becium grandiflorum lam. (lamiaceae) in streptozotocin-induced diabetic mice
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/5800ebe32c9f4364a2d187387ee328a0
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Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice

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