Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats

Abstract The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hieu Hoang Trung, Toru Yoshihara, Akito Nakao, Katsumi Hayashida, Yoshiki Hirata, Koumei Shirasuna, Mitsuru Kuwamura, Yuki Nakagawa, Takehito Kaneko, Yasuo Mori, Masahide Asano, Takashi Kuramoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5803a07e6fba4723b14dc1cd62900d0b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5803a07e6fba4723b14dc1cd62900d0b
record_format	dspace
spelling	oai:doaj.org-article:5803a07e6fba4723b14dc1cd62900d0b2021-12-02T10:49:29ZDeficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats10.1038/s41598-021-81515-x2045-2322https://doaj.org/article/5803a07e6fba4723b14dc1cd62900d0b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81515-xhttps://doaj.org/toc/2045-2322Abstract The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear conditioning tests. Here, we combined genetic, electrophysiological, and behavioral approaches to demonstrate that the RIβ subunit was involved in body tremor, LTP in the Schaffer collateral pathway, and fear conditioning memory in rats. Genetic analysis of WTC-furue, a mutant strain with spontaneous tremors, revealed a deletion in the Prkar1b gene of the WTC-furue genome. Prkar1b-deficient rats created by the CRISPR/Cas9 system exhibited body tremor. Hippocampal slices from mutant rats showed deficient LTP in the Schaffer collateral–CA1 synapse. Mutant rats also exhibited decreased freezing time following contextual and cued fear conditioning, as well as increased exploratory behavior in the open field. These findings indicate the roles of the RIβ subunit in tremor pathogenesis and contextual and cued fear memory, and suggest that the hippocampal and amygdala roles of this subunit differ between mice and rats and that rats are therefore beneficial for exploring RIβ function.Hieu Hoang TrungToru YoshiharaAkito NakaoKatsumi HayashidaYoshiki HirataKoumei ShirasunaMitsuru KuwamuraYuki NakagawaTakehito KanekoYasuo MoriMasahide AsanoTakashi KuramotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hieu Hoang Trung Toru Yoshihara Akito Nakao Katsumi Hayashida Yoshiki Hirata Koumei Shirasuna Mitsuru Kuwamura Yuki Nakagawa Takehito Kaneko Yasuo Mori Masahide Asano Takashi Kuramoto Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
description	Abstract The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear conditioning tests. Here, we combined genetic, electrophysiological, and behavioral approaches to demonstrate that the RIβ subunit was involved in body tremor, LTP in the Schaffer collateral pathway, and fear conditioning memory in rats. Genetic analysis of WTC-furue, a mutant strain with spontaneous tremors, revealed a deletion in the Prkar1b gene of the WTC-furue genome. Prkar1b-deficient rats created by the CRISPR/Cas9 system exhibited body tremor. Hippocampal slices from mutant rats showed deficient LTP in the Schaffer collateral–CA1 synapse. Mutant rats also exhibited decreased freezing time following contextual and cued fear conditioning, as well as increased exploratory behavior in the open field. These findings indicate the roles of the RIβ subunit in tremor pathogenesis and contextual and cued fear memory, and suggest that the hippocampal and amygdala roles of this subunit differ between mice and rats and that rats are therefore beneficial for exploring RIβ function.
format	article
author	Hieu Hoang Trung Toru Yoshihara Akito Nakao Katsumi Hayashida Yoshiki Hirata Koumei Shirasuna Mitsuru Kuwamura Yuki Nakagawa Takehito Kaneko Yasuo Mori Masahide Asano Takashi Kuramoto
author_facet	Hieu Hoang Trung Toru Yoshihara Akito Nakao Katsumi Hayashida Yoshiki Hirata Koumei Shirasuna Mitsuru Kuwamura Yuki Nakagawa Takehito Kaneko Yasuo Mori Masahide Asano Takashi Kuramoto
author_sort	Hieu Hoang Trung
title	Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
title_short	Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
title_full	Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
title_fullStr	Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
title_full_unstemmed	Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
title_sort	deficiency of the riβ subunit of protein kinase a causes body tremor and impaired fear conditioning memory in rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5803a07e6fba4723b14dc1cd62900d0b
work_keys_str_mv	AT hieuhoangtrung deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT toruyoshihara deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT akitonakao deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT katsumihayashida deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT yoshikihirata deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT koumeishirasuna deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT mitsurukuwamura deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT yukinakagawa deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT takehitokaneko deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT yasuomori deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT masahideasano deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats AT takashikuramoto deficiencyoftheribsubunitofproteinkinaseacausesbodytremorandimpairedfearconditioningmemoryinrats
_version_	1718396590740209664

Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats

Ejemplares similares