Leishmania metacyclogenesis is promoted in the absence of purines.
Leishmania parasites, the causative agent of leishmaniasis, are transmitted through the bite of an infected sand fly. Leishmania parasites present two basic forms known as promastigote and amastigote which, respectively, parasitizes the vector and the mammalian hosts. Infection of the vertebrate hos...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/5806430d98d24e3e98e9ff43318daddb |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:5806430d98d24e3e98e9ff43318daddb |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:5806430d98d24e3e98e9ff43318daddb2021-11-18T09:15:48ZLeishmania metacyclogenesis is promoted in the absence of purines.1935-27271935-273510.1371/journal.pntd.0001833https://doaj.org/article/5806430d98d24e3e98e9ff43318daddb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23050028/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Leishmania parasites, the causative agent of leishmaniasis, are transmitted through the bite of an infected sand fly. Leishmania parasites present two basic forms known as promastigote and amastigote which, respectively, parasitizes the vector and the mammalian hosts. Infection of the vertebrate host is dependent on the development, in the vector, of metacyclic promastigotes, however, little is known about the factors that trigger metacyclogenesis in Leishmania parasites. It has been generally stated that "stressful conditions" will lead to development of metacyclic forms, and with the exception of a few studies no detailed analysis of the molecular nature of the stress factor has been performed. Here we show that presence/absence of nucleosides, especially adenosine, controls metacyclogenesis both in vitro and in vivo. We found that addition of an adenosine-receptor antagonist to in vitro cultures of Leishmania amazonensis significantly increases metacyclogenesis, an effect that can be reversed by the presence of specific purine nucleosides or nucleobases. Furthermore, our results show that proliferation and metacyclogenesis are independently regulated and that addition of adenosine to culture medium is sufficient to recover proliferative characteristics for purified metacyclic promastigotes. More importantly, we show that metacyclogenesis was inhibited in sand flies infected with Leishmania infantum chagasi that were fed a mixture of sucrose and adenosine. Our results fill a gap in the life cycle of Leishmania parasites by demonstrating how metacyclogenesis, a key point in the propagation of the parasite to the mammalian host, can be controlled by the presence of specific purines.Tiago Donatelli SerafimAmanda Braga FigueiredoPedro Augusto Carvalho CostaEduardo Almeida Marques-da-SilvaRicardo GonçalvesSandra Aparecida Lima de MouraNelder Figueiredo GontijoSydnei Magno da SilvaMarilene Suzan Marques MichalickJosé Roberto Meyer-FernandesRoberto Paes de CarvalhoSilvia Reni Bortolin UlianaJuliana Lopes Rangel FiettoLuís Carlos Crocco AfonsoPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 6, Iss 9, p e1833 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
| spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Tiago Donatelli Serafim Amanda Braga Figueiredo Pedro Augusto Carvalho Costa Eduardo Almeida Marques-da-Silva Ricardo Gonçalves Sandra Aparecida Lima de Moura Nelder Figueiredo Gontijo Sydnei Magno da Silva Marilene Suzan Marques Michalick José Roberto Meyer-Fernandes Roberto Paes de Carvalho Silvia Reni Bortolin Uliana Juliana Lopes Rangel Fietto Luís Carlos Crocco Afonso Leishmania metacyclogenesis is promoted in the absence of purines. |
| description |
Leishmania parasites, the causative agent of leishmaniasis, are transmitted through the bite of an infected sand fly. Leishmania parasites present two basic forms known as promastigote and amastigote which, respectively, parasitizes the vector and the mammalian hosts. Infection of the vertebrate host is dependent on the development, in the vector, of metacyclic promastigotes, however, little is known about the factors that trigger metacyclogenesis in Leishmania parasites. It has been generally stated that "stressful conditions" will lead to development of metacyclic forms, and with the exception of a few studies no detailed analysis of the molecular nature of the stress factor has been performed. Here we show that presence/absence of nucleosides, especially adenosine, controls metacyclogenesis both in vitro and in vivo. We found that addition of an adenosine-receptor antagonist to in vitro cultures of Leishmania amazonensis significantly increases metacyclogenesis, an effect that can be reversed by the presence of specific purine nucleosides or nucleobases. Furthermore, our results show that proliferation and metacyclogenesis are independently regulated and that addition of adenosine to culture medium is sufficient to recover proliferative characteristics for purified metacyclic promastigotes. More importantly, we show that metacyclogenesis was inhibited in sand flies infected with Leishmania infantum chagasi that were fed a mixture of sucrose and adenosine. Our results fill a gap in the life cycle of Leishmania parasites by demonstrating how metacyclogenesis, a key point in the propagation of the parasite to the mammalian host, can be controlled by the presence of specific purines. |
| format |
article |
| author |
Tiago Donatelli Serafim Amanda Braga Figueiredo Pedro Augusto Carvalho Costa Eduardo Almeida Marques-da-Silva Ricardo Gonçalves Sandra Aparecida Lima de Moura Nelder Figueiredo Gontijo Sydnei Magno da Silva Marilene Suzan Marques Michalick José Roberto Meyer-Fernandes Roberto Paes de Carvalho Silvia Reni Bortolin Uliana Juliana Lopes Rangel Fietto Luís Carlos Crocco Afonso |
| author_facet |
Tiago Donatelli Serafim Amanda Braga Figueiredo Pedro Augusto Carvalho Costa Eduardo Almeida Marques-da-Silva Ricardo Gonçalves Sandra Aparecida Lima de Moura Nelder Figueiredo Gontijo Sydnei Magno da Silva Marilene Suzan Marques Michalick José Roberto Meyer-Fernandes Roberto Paes de Carvalho Silvia Reni Bortolin Uliana Juliana Lopes Rangel Fietto Luís Carlos Crocco Afonso |
| author_sort |
Tiago Donatelli Serafim |
| title |
Leishmania metacyclogenesis is promoted in the absence of purines. |
| title_short |
Leishmania metacyclogenesis is promoted in the absence of purines. |
| title_full |
Leishmania metacyclogenesis is promoted in the absence of purines. |
| title_fullStr |
Leishmania metacyclogenesis is promoted in the absence of purines. |
| title_full_unstemmed |
Leishmania metacyclogenesis is promoted in the absence of purines. |
| title_sort |
leishmania metacyclogenesis is promoted in the absence of purines. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/5806430d98d24e3e98e9ff43318daddb |
| work_keys_str_mv |
AT tiagodonatelliserafim leishmaniametacyclogenesisispromotedintheabsenceofpurines AT amandabragafigueiredo leishmaniametacyclogenesisispromotedintheabsenceofpurines AT pedroaugustocarvalhocosta leishmaniametacyclogenesisispromotedintheabsenceofpurines AT eduardoalmeidamarquesdasilva leishmaniametacyclogenesisispromotedintheabsenceofpurines AT ricardogoncalves leishmaniametacyclogenesisispromotedintheabsenceofpurines AT sandraaparecidalimademoura leishmaniametacyclogenesisispromotedintheabsenceofpurines AT nelderfigueiredogontijo leishmaniametacyclogenesisispromotedintheabsenceofpurines AT sydneimagnodasilva leishmaniametacyclogenesisispromotedintheabsenceofpurines AT marilenesuzanmarquesmichalick leishmaniametacyclogenesisispromotedintheabsenceofpurines AT joserobertomeyerfernandes leishmaniametacyclogenesisispromotedintheabsenceofpurines AT robertopaesdecarvalho leishmaniametacyclogenesisispromotedintheabsenceofpurines AT silviarenibortolinuliana leishmaniametacyclogenesisispromotedintheabsenceofpurines AT julianalopesrangelfietto leishmaniametacyclogenesisispromotedintheabsenceofpurines AT luiscarloscroccoafonso leishmaniametacyclogenesisispromotedintheabsenceofpurines |
| _version_ |
1718420904472477696 |