Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps

Abstract Producing industrially significant compounds with more environmentally friendly represents a challenging task. The large-scale production of an exogenous molecule in a host microfactory can quickly cause toxic effects, forcing the cell to inhibit production to survive. The key point to coun...

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Autores principales:	Erika Boulant, Emmanuelle Cambon, Julia Vergalli, Rémi Bernard, Fabienne Neulat-Ripoll, Flora Nolent, Olivier Gorgé, Maria Girleanu, Anne-Laure Favier, Jean-Paul Leonetti, Jean Michel Bolla
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/58071f4de37f4a468739d178ae7eda85
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spelling	oai:doaj.org-article:58071f4de37f4a468739d178ae7eda852021-12-02T12:11:40ZTolerance engineering in Deinococcus geothermalis by heterologous efflux pumps10.1038/s41598-021-83339-12045-2322https://doaj.org/article/58071f4de37f4a468739d178ae7eda852021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83339-1https://doaj.org/toc/2045-2322Abstract Producing industrially significant compounds with more environmentally friendly represents a challenging task. The large-scale production of an exogenous molecule in a host microfactory can quickly cause toxic effects, forcing the cell to inhibit production to survive. The key point to counter these toxic effects is to promote a gain of tolerance in the host, for instance, by inducing a constant flux of the neo-synthetized compound out of the producing cells. Efflux pumps are membrane proteins that constitute the most powerful mechanism to release molecules out of cells. We propose here a new biological model, Deinococcus geothermalis, organism known for its ability to survive hostile environment; with the aim of coupling the promising industrial potential of this species with that of heterologous efflux pumps to promote engineering tolerance. In this study, clones of D. geothermalis containing various genes encoding chromosomal heterologous efflux pumps were generated. Resistant recombinants were selected using antibiotic susceptibility tests to screen promising candidates. We then developed a method to determine the efflux efficiency of the best candidate, which contains the gene encoding the MdfA of Salmonella enterica serovar Choleraesuis. We observe 1.6 times more compound in the external medium of the hit recombinant than that of the WT at early incubation time. The data presented here will contribute to better understanding of the parameters required for efficient production in D. geothermalis.Erika BoulantEmmanuelle CambonJulia VergalliRémi BernardFabienne Neulat-RipollFlora NolentOlivier GorgéMaria GirleanuAnne-Laure FavierJean-Paul LeonettiJean Michel BollaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erika Boulant Emmanuelle Cambon Julia Vergalli Rémi Bernard Fabienne Neulat-Ripoll Flora Nolent Olivier Gorgé Maria Girleanu Anne-Laure Favier Jean-Paul Leonetti Jean Michel Bolla Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps
description	Abstract Producing industrially significant compounds with more environmentally friendly represents a challenging task. The large-scale production of an exogenous molecule in a host microfactory can quickly cause toxic effects, forcing the cell to inhibit production to survive. The key point to counter these toxic effects is to promote a gain of tolerance in the host, for instance, by inducing a constant flux of the neo-synthetized compound out of the producing cells. Efflux pumps are membrane proteins that constitute the most powerful mechanism to release molecules out of cells. We propose here a new biological model, Deinococcus geothermalis, organism known for its ability to survive hostile environment; with the aim of coupling the promising industrial potential of this species with that of heterologous efflux pumps to promote engineering tolerance. In this study, clones of D. geothermalis containing various genes encoding chromosomal heterologous efflux pumps were generated. Resistant recombinants were selected using antibiotic susceptibility tests to screen promising candidates. We then developed a method to determine the efflux efficiency of the best candidate, which contains the gene encoding the MdfA of Salmonella enterica serovar Choleraesuis. We observe 1.6 times more compound in the external medium of the hit recombinant than that of the WT at early incubation time. The data presented here will contribute to better understanding of the parameters required for efficient production in D. geothermalis.
format	article
author	Erika Boulant Emmanuelle Cambon Julia Vergalli Rémi Bernard Fabienne Neulat-Ripoll Flora Nolent Olivier Gorgé Maria Girleanu Anne-Laure Favier Jean-Paul Leonetti Jean Michel Bolla
author_facet	Erika Boulant Emmanuelle Cambon Julia Vergalli Rémi Bernard Fabienne Neulat-Ripoll Flora Nolent Olivier Gorgé Maria Girleanu Anne-Laure Favier Jean-Paul Leonetti Jean Michel Bolla
author_sort	Erika Boulant
title	Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps
title_short	Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps
title_full	Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps
title_fullStr	Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps
title_full_unstemmed	Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps
title_sort	tolerance engineering in deinococcus geothermalis by heterologous efflux pumps
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/58071f4de37f4a468739d178ae7eda85
work_keys_str_mv	AT erikaboulant toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT emmanuellecambon toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT juliavergalli toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT remibernard toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT fabienneneulatripoll toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT floranolent toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT oliviergorge toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT mariagirleanu toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT annelaurefavier toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT jeanpaulleonetti toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps AT jeanmichelbolla toleranceengineeringindeinococcusgeothermalisbyheterologouseffluxpumps
_version_	1718394629580128256

Tolerance engineering in Deinococcus geothermalis by heterologous efflux pumps

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