Suppression of diabetic retinopathy with GLUT1 siRNA

Abstract To investigate the effect of glucose transporter-1 (GLUT1) inhibition on diabetic retinopathy, we divided forty-eight mice into scrambled siRNA, diabetic scrambled siRNA, and GLUT1 siRNA (intravitreally injected) groups. Twenty-one weeks after diabetes induction, we calculated retinal gluco...

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Autores principales:	Zhi-Peng You, Yu-Lan Zhang, Ke Shi, Lu Shi, Yue-Zhi Zhang, Yue Zhou, Chang-yun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5808eecaf5b14088adeec0c19480589a
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spelling	oai:doaj.org-article:5808eecaf5b14088adeec0c19480589a2021-12-02T15:05:26ZSuppression of diabetic retinopathy with GLUT1 siRNA10.1038/s41598-017-07942-x2045-2322https://doaj.org/article/5808eecaf5b14088adeec0c19480589a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07942-xhttps://doaj.org/toc/2045-2322Abstract To investigate the effect of glucose transporter-1 (GLUT1) inhibition on diabetic retinopathy, we divided forty-eight mice into scrambled siRNA, diabetic scrambled siRNA, and GLUT1 siRNA (intravitreally injected) groups. Twenty-one weeks after diabetes induction, we calculated retinal glucose concentrations, used electroretinography (ERG) and histochemical methods to assess photoreceptor degeneration, and conducted immunoblotting, leukostasis and vascular leakage assays to estimate microangiopathy. The diabetic scrambled siRNA and GLUT1 siRNA exhibited higher glucose concentrations than scrambled siRNA, but GLUT1 siRNA group concentrations were only 50.05% of diabetic scrambled siRNA due to downregulated GLUT1 expression. The diabetic scrambled siRNA and GLUT1 siRNA had lower ERG amplitudes and ONL thicknesses than scrambled siRNA. However, compared with diabetic scrambled siRNA, GLUT1 siRNA group amplitudes and thicknesses were higher. Diabetic scrambled siRNA cones were more loosely arranged and had shorter outer segments than GLUT1 siRNA cones. ICAM-1 and TNF-α expression levels, adherent leukocyte numbers, fluorescence leakage areas and extravasated Evans blue in diabetic scrambled siRNA were higher than those in scrambled siRNA. However, these parameters in the GLUT1 siRNA were lower than diabetic scrambled siRNA. Together, these results demonstrate that GLUT1 siRNA restricted glucose transport by inhibiting GLUT1 expression, which decreased retinal glucose concentrations and ameliorated diabetic retinopathy.Zhi-Peng YouYu-Lan ZhangKe ShiLu ShiYue-Zhi ZhangYue ZhouChang-yun WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhi-Peng You Yu-Lan Zhang Ke Shi Lu Shi Yue-Zhi Zhang Yue Zhou Chang-yun Wang Suppression of diabetic retinopathy with GLUT1 siRNA
description	Abstract To investigate the effect of glucose transporter-1 (GLUT1) inhibition on diabetic retinopathy, we divided forty-eight mice into scrambled siRNA, diabetic scrambled siRNA, and GLUT1 siRNA (intravitreally injected) groups. Twenty-one weeks after diabetes induction, we calculated retinal glucose concentrations, used electroretinography (ERG) and histochemical methods to assess photoreceptor degeneration, and conducted immunoblotting, leukostasis and vascular leakage assays to estimate microangiopathy. The diabetic scrambled siRNA and GLUT1 siRNA exhibited higher glucose concentrations than scrambled siRNA, but GLUT1 siRNA group concentrations were only 50.05% of diabetic scrambled siRNA due to downregulated GLUT1 expression. The diabetic scrambled siRNA and GLUT1 siRNA had lower ERG amplitudes and ONL thicknesses than scrambled siRNA. However, compared with diabetic scrambled siRNA, GLUT1 siRNA group amplitudes and thicknesses were higher. Diabetic scrambled siRNA cones were more loosely arranged and had shorter outer segments than GLUT1 siRNA cones. ICAM-1 and TNF-α expression levels, adherent leukocyte numbers, fluorescence leakage areas and extravasated Evans blue in diabetic scrambled siRNA were higher than those in scrambled siRNA. However, these parameters in the GLUT1 siRNA were lower than diabetic scrambled siRNA. Together, these results demonstrate that GLUT1 siRNA restricted glucose transport by inhibiting GLUT1 expression, which decreased retinal glucose concentrations and ameliorated diabetic retinopathy.
format	article
author	Zhi-Peng You Yu-Lan Zhang Ke Shi Lu Shi Yue-Zhi Zhang Yue Zhou Chang-yun Wang
author_facet	Zhi-Peng You Yu-Lan Zhang Ke Shi Lu Shi Yue-Zhi Zhang Yue Zhou Chang-yun Wang
author_sort	Zhi-Peng You
title	Suppression of diabetic retinopathy with GLUT1 siRNA
title_short	Suppression of diabetic retinopathy with GLUT1 siRNA
title_full	Suppression of diabetic retinopathy with GLUT1 siRNA
title_fullStr	Suppression of diabetic retinopathy with GLUT1 siRNA
title_full_unstemmed	Suppression of diabetic retinopathy with GLUT1 siRNA
title_sort	suppression of diabetic retinopathy with glut1 sirna
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5808eecaf5b14088adeec0c19480589a
work_keys_str_mv	AT zhipengyou suppressionofdiabeticretinopathywithglut1sirna AT yulanzhang suppressionofdiabeticretinopathywithglut1sirna AT keshi suppressionofdiabeticretinopathywithglut1sirna AT lushi suppressionofdiabeticretinopathywithglut1sirna AT yuezhizhang suppressionofdiabeticretinopathywithglut1sirna AT yuezhou suppressionofdiabeticretinopathywithglut1sirna AT changyunwang suppressionofdiabeticretinopathywithglut1sirna
_version_	1718388830545903616

Suppression of diabetic retinopathy with GLUT1 siRNA

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