Stac3 inhibits myoblast differentiation into myotubes.

The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. In this study we determined the potential role of Stac3 i...

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Autores principales:	Xiaomei Ge, Yafei Zhang, Sungwon Park, Xiaofei Cong, David E Gerrard, Honglin Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/580bca21b607494396f04a1d9cafc06d
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spelling	oai:doaj.org-article:580bca21b607494396f04a1d9cafc06d2021-11-18T08:21:18ZStac3 inhibits myoblast differentiation into myotubes.1932-620310.1371/journal.pone.0095926https://doaj.org/article/580bca21b607494396f04a1d9cafc06d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24788338/?tool=EBIhttps://doaj.org/toc/1932-6203The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. In contrast, Stac3 overexpression inhibited the differentiation of C2C12 myoblasts into myotubes as evidenced by decreased fusion index, decreased number of nuclei per myotube, and decreased mRNA and protein expression of myogenic markers. Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. Collectively, these data suggest an inhibitory role of endogenous Stac3 in myoblast differentiation. Myogenesis is a tightly controlled program; myofibers formed from prematurely differentiated myoblasts are dysfunctional. Thus, Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development.Xiaomei GeYafei ZhangSungwon ParkXiaofei CongDavid E GerrardHonglin JiangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e95926 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaomei Ge Yafei Zhang Sungwon Park Xiaofei Cong David E Gerrard Honglin Jiang Stac3 inhibits myoblast differentiation into myotubes.
description	The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. In contrast, Stac3 overexpression inhibited the differentiation of C2C12 myoblasts into myotubes as evidenced by decreased fusion index, decreased number of nuclei per myotube, and decreased mRNA and protein expression of myogenic markers. Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. Collectively, these data suggest an inhibitory role of endogenous Stac3 in myoblast differentiation. Myogenesis is a tightly controlled program; myofibers formed from prematurely differentiated myoblasts are dysfunctional. Thus, Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development.
format	article
author	Xiaomei Ge Yafei Zhang Sungwon Park Xiaofei Cong David E Gerrard Honglin Jiang
author_facet	Xiaomei Ge Yafei Zhang Sungwon Park Xiaofei Cong David E Gerrard Honglin Jiang
author_sort	Xiaomei Ge
title	Stac3 inhibits myoblast differentiation into myotubes.
title_short	Stac3 inhibits myoblast differentiation into myotubes.
title_full	Stac3 inhibits myoblast differentiation into myotubes.
title_fullStr	Stac3 inhibits myoblast differentiation into myotubes.
title_full_unstemmed	Stac3 inhibits myoblast differentiation into myotubes.
title_sort	stac3 inhibits myoblast differentiation into myotubes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/580bca21b607494396f04a1d9cafc06d
work_keys_str_mv	AT xiaomeige stac3inhibitsmyoblastdifferentiationintomyotubes AT yafeizhang stac3inhibitsmyoblastdifferentiationintomyotubes AT sungwonpark stac3inhibitsmyoblastdifferentiationintomyotubes AT xiaofeicong stac3inhibitsmyoblastdifferentiationintomyotubes AT davidegerrard stac3inhibitsmyoblastdifferentiationintomyotubes AT honglinjiang stac3inhibitsmyoblastdifferentiationintomyotubes
_version_	1718421861778325504

Stac3 inhibits myoblast differentiation into myotubes.

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