Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice

Background. It is important to improve the understanding of the fracture healing process at the molecular levels, then to discover potential miRNA regulatory mechanisms and candidate markers. Methods. Expression profiles of mRNA and miRNA were obtained from the Gene Expression Omnibus database. We p...

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Autores principales:	Xianglu Li, Zhaohua Zhong, Enguang Ma, Xiaowei Wu
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Computer applications to medicine. Medical informatics R858-859.7
Acceso en línea:	https://doaj.org/article/581015601b754b71b05a32b431384539
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spelling	oai:doaj.org-article:581015601b754b71b05a32b4313845392021-11-29T00:55:52ZIdentification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice1748-671810.1155/2021/2866475https://doaj.org/article/581015601b754b71b05a32b4313845392021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/2866475https://doaj.org/toc/1748-6718Background. It is important to improve the understanding of the fracture healing process at the molecular levels, then to discover potential miRNA regulatory mechanisms and candidate markers. Methods. Expression profiles of mRNA and miRNA were obtained from the Gene Expression Omnibus database. We performed differential analysis, enrichment analysis, protein-protein interaction (PPI) network analysis. The miRNA-mRNA network analysis was also performed. Results. We identified 499 differentially expressed mRNAs (DEmRs) that were upregulated and 534 downregulated DEmRs during fracture healing. They were mainly enriched in collagen fibril organization and immune response. Using the PPI network, we screened 10 hub genes that were upregulated and 10 hub genes downregulated with the largest connectivity. We further constructed the miRNA regulatory network for hub genes and identified 13 differentially expressed miRNAs (DEmiRs) regulators. Cd19 and Col6a1 were identified as key candidate mRNAs with the largest fold change, and their DEmiR regulators were key candidate regulators. Conclusion. Cd19 and Col6a1 might serve as candidate markers for fracture healing in subsequent studies. Their expression is regulated by miRNAs and is involved in collagen fibril organization and immune responses.Xianglu LiZhaohua ZhongEnguang MaXiaowei WuHindawi LimitedarticleComputer applications to medicine. Medical informaticsR858-859.7ENComputational and Mathematical Methods in Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Computer applications to medicine. Medical informatics R858-859.7
spellingShingle	Computer applications to medicine. Medical informatics R858-859.7 Xianglu Li Zhaohua Zhong Enguang Ma Xiaowei Wu Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice
description	Background. It is important to improve the understanding of the fracture healing process at the molecular levels, then to discover potential miRNA regulatory mechanisms and candidate markers. Methods. Expression profiles of mRNA and miRNA were obtained from the Gene Expression Omnibus database. We performed differential analysis, enrichment analysis, protein-protein interaction (PPI) network analysis. The miRNA-mRNA network analysis was also performed. Results. We identified 499 differentially expressed mRNAs (DEmRs) that were upregulated and 534 downregulated DEmRs during fracture healing. They were mainly enriched in collagen fibril organization and immune response. Using the PPI network, we screened 10 hub genes that were upregulated and 10 hub genes downregulated with the largest connectivity. We further constructed the miRNA regulatory network for hub genes and identified 13 differentially expressed miRNAs (DEmiRs) regulators. Cd19 and Col6a1 were identified as key candidate mRNAs with the largest fold change, and their DEmiR regulators were key candidate regulators. Conclusion. Cd19 and Col6a1 might serve as candidate markers for fracture healing in subsequent studies. Their expression is regulated by miRNAs and is involved in collagen fibril organization and immune responses.
format	article
author	Xianglu Li Zhaohua Zhong Enguang Ma Xiaowei Wu
author_facet	Xianglu Li Zhaohua Zhong Enguang Ma Xiaowei Wu
author_sort	Xianglu Li
title	Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice
title_short	Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice
title_full	Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice
title_fullStr	Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice
title_full_unstemmed	Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice
title_sort	identification of mirna regulatory networks and candidate markers for fracture healing in mice
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/581015601b754b71b05a32b431384539
work_keys_str_mv	AT xiangluli identificationofmirnaregulatorynetworksandcandidatemarkersforfracturehealinginmice AT zhaohuazhong identificationofmirnaregulatorynetworksandcandidatemarkersforfracturehealinginmice AT enguangma identificationofmirnaregulatorynetworksandcandidatemarkersforfracturehealinginmice AT xiaoweiwu identificationofmirnaregulatorynetworksandcandidatemarkersforfracturehealinginmice
_version_	1718407769179029504

Identification of miRNA Regulatory Networks and Candidate Markers for Fracture Healing in Mice

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