Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes

Abstract The Nucleocapsid Protein (N Protein) of severe acute respiratory syndrome Coronavirus 2 (SARS-CoV2) is located in the viral core. Immunoglobulin G (IgG) targeting N protein is detectable in the serum of infected patients. The effect of high titers of IgG against N-protein on clinical outcom...

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Autores principales:	Mayank Batra, Runxia Tian, Chongxu Zhang, Emile Clarence, Camila Sofia Sacher, Justin Nestor Miranda, Justin Rafa O. De La Fuente, Megan Mathew, Desmond Green, Sayari Patel, Maria Virginia Perez Bastidas, Sara Haddadi, Mukunthan Murthi, Miguel Santiago Gonzalez, Shweta Kambali, Kayo H. M. Santos, Huda Asif, Farzaneh Modarresi, Mohammad Faghihi, Mehdi Mirsaeidi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/581137ccf5854a13a0c73fa00760ace2
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spelling	oai:doaj.org-article:581137ccf5854a13a0c73fa00760ace22021-12-02T13:30:10ZRole of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes10.1038/s41598-021-83108-02045-2322https://doaj.org/article/581137ccf5854a13a0c73fa00760ace22021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83108-0https://doaj.org/toc/2045-2322Abstract The Nucleocapsid Protein (N Protein) of severe acute respiratory syndrome Coronavirus 2 (SARS-CoV2) is located in the viral core. Immunoglobulin G (IgG) targeting N protein is detectable in the serum of infected patients. The effect of high titers of IgG against N-protein on clinical outcomes of SARS-CoV2 disease has not been described. We studied 400 RT-PCR confirmed SARS-CoV2 patients to determine independent factors associated with poor outcomes, including Medical Intensive Care Unit (MICU) admission, prolonged MICU stay and hospital admissions, and in-hospital mortality. We also measured serum IgG against the N protein and correlated its concentrations with clinical outcomes. We found that several factors, including Charlson comorbidity Index (CCI), high levels of IL6, and presentation with dyspnea were associated with poor clinical outcomes. It was shown that higher CCI and higher IL6 levels were independently associated with in-hospital mortality. Anti-N protein IgG was detected in the serum of 55 (55%) patients at the time of admission. A high concentration of antibodies, defined as signal to cut off ratio (S/Co) > 1.5 (75 percentile of all measurements), was found in 25 (25%) patients. The multivariable logistic regression models showed that between being an African American, higher CCI, lymphocyte counts, and S/Co ratio > 1.5, only S/Co ratio were independently associated with MICU admission and longer length of stay in hospital. This study recommends that titers of IgG targeting N-protein of SARS-CoV2 at admission is a prognostic factor for the clinical course of disease and should be measured in all patients with SARS-CoV2 infection.Mayank BatraRunxia TianChongxu ZhangEmile ClarenceCamila Sofia SacherJustin Nestor MirandaJustin Rafa O. De La FuenteMegan MathewDesmond GreenSayari PatelMaria Virginia Perez BastidasSara HaddadiMukunthan MurthiMiguel Santiago GonzalezShweta KambaliKayo H. M. SantosHuda AsifFarzaneh ModarresiMohammad FaghihiMehdi MirsaeidiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mayank Batra Runxia Tian Chongxu Zhang Emile Clarence Camila Sofia Sacher Justin Nestor Miranda Justin Rafa O. De La Fuente Megan Mathew Desmond Green Sayari Patel Maria Virginia Perez Bastidas Sara Haddadi Mukunthan Murthi Miguel Santiago Gonzalez Shweta Kambali Kayo H. M. Santos Huda Asif Farzaneh Modarresi Mohammad Faghihi Mehdi Mirsaeidi Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes
description	Abstract The Nucleocapsid Protein (N Protein) of severe acute respiratory syndrome Coronavirus 2 (SARS-CoV2) is located in the viral core. Immunoglobulin G (IgG) targeting N protein is detectable in the serum of infected patients. The effect of high titers of IgG against N-protein on clinical outcomes of SARS-CoV2 disease has not been described. We studied 400 RT-PCR confirmed SARS-CoV2 patients to determine independent factors associated with poor outcomes, including Medical Intensive Care Unit (MICU) admission, prolonged MICU stay and hospital admissions, and in-hospital mortality. We also measured serum IgG against the N protein and correlated its concentrations with clinical outcomes. We found that several factors, including Charlson comorbidity Index (CCI), high levels of IL6, and presentation with dyspnea were associated with poor clinical outcomes. It was shown that higher CCI and higher IL6 levels were independently associated with in-hospital mortality. Anti-N protein IgG was detected in the serum of 55 (55%) patients at the time of admission. A high concentration of antibodies, defined as signal to cut off ratio (S/Co) > 1.5 (75 percentile of all measurements), was found in 25 (25%) patients. The multivariable logistic regression models showed that between being an African American, higher CCI, lymphocyte counts, and S/Co ratio > 1.5, only S/Co ratio were independently associated with MICU admission and longer length of stay in hospital. This study recommends that titers of IgG targeting N-protein of SARS-CoV2 at admission is a prognostic factor for the clinical course of disease and should be measured in all patients with SARS-CoV2 infection.
format	article
author	Mayank Batra Runxia Tian Chongxu Zhang Emile Clarence Camila Sofia Sacher Justin Nestor Miranda Justin Rafa O. De La Fuente Megan Mathew Desmond Green Sayari Patel Maria Virginia Perez Bastidas Sara Haddadi Mukunthan Murthi Miguel Santiago Gonzalez Shweta Kambali Kayo H. M. Santos Huda Asif Farzaneh Modarresi Mohammad Faghihi Mehdi Mirsaeidi
author_facet	Mayank Batra Runxia Tian Chongxu Zhang Emile Clarence Camila Sofia Sacher Justin Nestor Miranda Justin Rafa O. De La Fuente Megan Mathew Desmond Green Sayari Patel Maria Virginia Perez Bastidas Sara Haddadi Mukunthan Murthi Miguel Santiago Gonzalez Shweta Kambali Kayo H. M. Santos Huda Asif Farzaneh Modarresi Mohammad Faghihi Mehdi Mirsaeidi
author_sort	Mayank Batra
title	Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes
title_short	Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes
title_full	Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes
title_fullStr	Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes
title_full_unstemmed	Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes
title_sort	role of igg against n-protein of sars-cov2 in covid19 clinical outcomes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/581137ccf5854a13a0c73fa00760ace2
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Role of IgG against N-protein of SARS-CoV2 in COVID19 clinical outcomes

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