Norovirus P particle efficiently elicits innate, humoral and cellular immunity.

Norovirus (NoV) P domain complexes, the 24 mer P particles and the P dimers, induced effective humoral immunity, but their role in the cellular immune responses remained unclear. We reported here a study on cellular immune responses of the two P domain complexes in comparison with the virus-like par...

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Autores principales:	Hao Fang, Ming Tan, Ming Xia, Leyi Wang, Xi Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5816f14cf91d4188b5e06df3edb8fbd3
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spelling	oai:doaj.org-article:5816f14cf91d4188b5e06df3edb8fbd32021-11-18T07:47:35ZNorovirus P particle efficiently elicits innate, humoral and cellular immunity.1932-620310.1371/journal.pone.0063269https://doaj.org/article/5816f14cf91d4188b5e06df3edb8fbd32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638188/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Norovirus (NoV) P domain complexes, the 24 mer P particles and the P dimers, induced effective humoral immunity, but their role in the cellular immune responses remained unclear. We reported here a study on cellular immune responses of the two P domain complexes in comparison with the virus-like particle (VLP) of a GII.4 NoV (VA387) in mice. The P domain complexes induced significant central memory CD4(+) T cell phenotypes (CD4(+) CD44(+) CD62L(+) CCR7(+)) and activated polyclonal CD4(+) T cells as shown by production of Interleukin (IL)-2, Interferon (IFN)-γ, and Tumor Necrosis Factor (TNF)-α. Most importantly, VA387-specific CD4(+) T cell epitope induced a production of IFN-γ, indicating an antigen-specific CD4(+) T cell response in P domain complex-immunized mice. Furthermore, P domain complexes efficiently induced bone marrow-derived dendritic cell (BMDC) maturation, evidenced by up-regulation of co-stimulatory and MHC class II molecules, as well as production of IL-12 and IL-1β. Finally, P domain complex-induced mature dendritic cells (DCs) elicited proliferation of specific CD4(+) T cells targeting VA387 P domain. Overall, we conclude that the NoV P domain complexes are efficiently presented by DCs to elicit not only humoral but also cellular immune responses against NoVs. Since the P particle is highly effective for both humoral and cellular immune responses and easily produced in Escherichia coli (E. coli), it is a good choice of vaccine against NoVs and a vaccine platform against other diseases.Hao FangMing TanMing XiaLeyi WangXi JiangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e63269 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hao Fang Ming Tan Ming Xia Leyi Wang Xi Jiang Norovirus P particle efficiently elicits innate, humoral and cellular immunity.
description	Norovirus (NoV) P domain complexes, the 24 mer P particles and the P dimers, induced effective humoral immunity, but their role in the cellular immune responses remained unclear. We reported here a study on cellular immune responses of the two P domain complexes in comparison with the virus-like particle (VLP) of a GII.4 NoV (VA387) in mice. The P domain complexes induced significant central memory CD4(+) T cell phenotypes (CD4(+) CD44(+) CD62L(+) CCR7(+)) and activated polyclonal CD4(+) T cells as shown by production of Interleukin (IL)-2, Interferon (IFN)-γ, and Tumor Necrosis Factor (TNF)-α. Most importantly, VA387-specific CD4(+) T cell epitope induced a production of IFN-γ, indicating an antigen-specific CD4(+) T cell response in P domain complex-immunized mice. Furthermore, P domain complexes efficiently induced bone marrow-derived dendritic cell (BMDC) maturation, evidenced by up-regulation of co-stimulatory and MHC class II molecules, as well as production of IL-12 and IL-1β. Finally, P domain complex-induced mature dendritic cells (DCs) elicited proliferation of specific CD4(+) T cells targeting VA387 P domain. Overall, we conclude that the NoV P domain complexes are efficiently presented by DCs to elicit not only humoral but also cellular immune responses against NoVs. Since the P particle is highly effective for both humoral and cellular immune responses and easily produced in Escherichia coli (E. coli), it is a good choice of vaccine against NoVs and a vaccine platform against other diseases.
format	article
author	Hao Fang Ming Tan Ming Xia Leyi Wang Xi Jiang
author_facet	Hao Fang Ming Tan Ming Xia Leyi Wang Xi Jiang
author_sort	Hao Fang
title	Norovirus P particle efficiently elicits innate, humoral and cellular immunity.
title_short	Norovirus P particle efficiently elicits innate, humoral and cellular immunity.
title_full	Norovirus P particle efficiently elicits innate, humoral and cellular immunity.
title_fullStr	Norovirus P particle efficiently elicits innate, humoral and cellular immunity.
title_full_unstemmed	Norovirus P particle efficiently elicits innate, humoral and cellular immunity.
title_sort	norovirus p particle efficiently elicits innate, humoral and cellular immunity.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5816f14cf91d4188b5e06df3edb8fbd3
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Norovirus P particle efficiently elicits innate, humoral and cellular immunity.

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