Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29
Azo dyes are used for silk textile manufacture, where their decolorization and detoxication are necessary after initial dying in the craft industry. The biodecolorization efficiency of Streptomyces coelicoflavus CS-29 toward commercial azo blue and red dyes was investigated, analyzing the degrada...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Mahidol University
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/5818d3b923d0481fb585f020132f7e2d |
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| Sumario: | Azo dyes are used for silk textile manufacture, where their decolorization and
detoxication are necessary after initial dying in the craft industry. The biodecolorization
efficiency of Streptomyces coelicoflavus CS-29 toward commercial
azo blue and red dyes was investigated, analyzing the degradation and adsorption
of dye molecules. S. coelicoflavus CS-29 showed reductions of 70% and 51% in
red and blue dyes, respectively, after seven days. Morphological observation by
light microscopy showed that dye molecules were adsorbed onto S. coelicoflavus
CS-29 cell surface to form a dense cell pellet. Moreover, peroxidase and laccase
activity were detected as extracellular enzymes, but no azo-reductase was detected.
From the enzymatic activity, changes of dye profiles in HPLC showed differences
between control dyes (untreated dyes) and metabolized products of dyes treated
with S. coelicoflavus CS-29. The presence of main functional azo groups (-N=N-)
in both blue and red silk dyes was indicated by FTIR analysis, in the untreated azo
dyes. The azo bonds seemed to disappear in metabolites after S. coelicoflavus CS-
29 treatment and other functional groups were changed compared to the control
dyes. The treated dyes showed no significant effect on seed germination, root
length, and shoot length of mung beans during phytotoxicity analysis. The red dyes
showed a more negative effect on shoot lengths than the blue dyes. The overall
results showed that S. coelicoflavus CS-29 is an effective and promising tool for
the treatment of dye contaminated wastewater and the permanent elimination of
recalcitrant commercial azo dye pollutants. |
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