Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29

Azo dyes are used for silk textile manufacture, where their decolorization and detoxication are necessary after initial dying in the craft industry. The biodecolorization efficiency of Streptomyces coelicoflavus CS-29 toward commercial azo blue and red dyes was investigated, analyzing the degrada...

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Autores principales:	Wai Phyo Mon, Phongphan Jantaharn, Sophon Boonlue, Sirirath McCloskey, Somdej Kanokmedhakul, Wiyada Mongkolthanaruk
Formato:	article
Lenguaje:	EN
Publicado:	Mahidol University 2021
Materias:	azo silk dyes biosorption decolorization degradation Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/5818d3b923d0481fb585f020132f7e2d
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spelling	oai:doaj.org-article:5818d3b923d0481fb585f020132f7e2d2021-12-03T04:07:56ZEnzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-2910.32526/ennrj/20/2021001041686-54562408-2384https://doaj.org/article/5818d3b923d0481fb585f020132f7e2d2021-11-01T00:00:00Zhttps://ph02.tci-thaijo.org/index.php/ennrj/article/view/245151/166230https://doaj.org/toc/1686-5456https://doaj.org/toc/2408-2384Azo dyes are used for silk textile manufacture, where their decolorization and detoxication are necessary after initial dying in the craft industry. The biodecolorization efficiency of Streptomyces coelicoflavus CS-29 toward commercial azo blue and red dyes was investigated, analyzing the degradation and adsorption of dye molecules. S. coelicoflavus CS-29 showed reductions of 70% and 51% in red and blue dyes, respectively, after seven days. Morphological observation by light microscopy showed that dye molecules were adsorbed onto S. coelicoflavus CS-29 cell surface to form a dense cell pellet. Moreover, peroxidase and laccase activity were detected as extracellular enzymes, but no azo-reductase was detected. From the enzymatic activity, changes of dye profiles in HPLC showed differences between control dyes (untreated dyes) and metabolized products of dyes treated with S. coelicoflavus CS-29. The presence of main functional azo groups (-N=N-) in both blue and red silk dyes was indicated by FTIR analysis, in the untreated azo dyes. The azo bonds seemed to disappear in metabolites after S. coelicoflavus CS- 29 treatment and other functional groups were changed compared to the control dyes. The treated dyes showed no significant effect on seed germination, root length, and shoot length of mung beans during phytotoxicity analysis. The red dyes showed a more negative effect on shoot lengths than the blue dyes. The overall results showed that S. coelicoflavus CS-29 is an effective and promising tool for the treatment of dye contaminated wastewater and the permanent elimination of recalcitrant commercial azo dye pollutants.Wai Phyo MonPhongphan JantaharnSophon BoonlueSirirath McCloskeySomdej KanokmedhakulWiyada MongkolthanarukMahidol Universityarticleazo silk dyesbiosorptiondecolorizationdegradationEnvironmental technology. Sanitary engineeringTD1-1066Environmental sciencesGE1-350ENEnvironment and Natural Resources Journal, Vol 20, Iss 1, Pp 19-28 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	azo silk dyes biosorption decolorization degradation Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350
spellingShingle	azo silk dyes biosorption decolorization degradation Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350 Wai Phyo Mon Phongphan Jantaharn Sophon Boonlue Sirirath McCloskey Somdej Kanokmedhakul Wiyada Mongkolthanaruk Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29
description	Azo dyes are used for silk textile manufacture, where their decolorization and detoxication are necessary after initial dying in the craft industry. The biodecolorization efficiency of Streptomyces coelicoflavus CS-29 toward commercial azo blue and red dyes was investigated, analyzing the degradation and adsorption of dye molecules. S. coelicoflavus CS-29 showed reductions of 70% and 51% in red and blue dyes, respectively, after seven days. Morphological observation by light microscopy showed that dye molecules were adsorbed onto S. coelicoflavus CS-29 cell surface to form a dense cell pellet. Moreover, peroxidase and laccase activity were detected as extracellular enzymes, but no azo-reductase was detected. From the enzymatic activity, changes of dye profiles in HPLC showed differences between control dyes (untreated dyes) and metabolized products of dyes treated with S. coelicoflavus CS-29. The presence of main functional azo groups (-N=N-) in both blue and red silk dyes was indicated by FTIR analysis, in the untreated azo dyes. The azo bonds seemed to disappear in metabolites after S. coelicoflavus CS- 29 treatment and other functional groups were changed compared to the control dyes. The treated dyes showed no significant effect on seed germination, root length, and shoot length of mung beans during phytotoxicity analysis. The red dyes showed a more negative effect on shoot lengths than the blue dyes. The overall results showed that S. coelicoflavus CS-29 is an effective and promising tool for the treatment of dye contaminated wastewater and the permanent elimination of recalcitrant commercial azo dye pollutants.
format	article
author	Wai Phyo Mon Phongphan Jantaharn Sophon Boonlue Sirirath McCloskey Somdej Kanokmedhakul Wiyada Mongkolthanaruk
author_facet	Wai Phyo Mon Phongphan Jantaharn Sophon Boonlue Sirirath McCloskey Somdej Kanokmedhakul Wiyada Mongkolthanaruk
author_sort	Wai Phyo Mon
title	Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29
title_short	Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29
title_full	Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29
title_fullStr	Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29
title_full_unstemmed	Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29
title_sort	enzymatic degradation of azo bonds and other functional groups on commercial silk dyes by streptomyces coelicoflavus cs-29
publisher	Mahidol University
publishDate	2021
url	https://doaj.org/article/5818d3b923d0481fb585f020132f7e2d
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Enzymatic Degradation of Azo Bonds and Other Functional Groups on Commercial Silk Dyes by Streptomyces coelicoflavus CS-29

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