Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children

Abstract Tuberculosis (TB) is a major cause of morbidity and mortality in children, and early diagnosis and treatment are crucial to reduce long-term morbidity and mortality. In this study, we explore whether urine nuclear magnetic resonance (NMR)-based metabolomics could be used to identify differe...

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Autores principales:	Patricia Comella-del-Barrio, José Luis Izquierdo-Garcia, Jacqueline Gautier, Mariette Jean Coute Doresca, Ramón Campos-Olivas, Clara M. Santiveri, Beatriz Muriel-Moreno, Cristina Prat-Aymerich, Rosa Abellana, Tomas M. Pérez-Porcuna, Luis E. Cuevas, Jesús Ruiz-Cabello, José Domínguez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5819bbdaba1642aca78d88c48e191687
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spelling	oai:doaj.org-article:5819bbdaba1642aca78d88c48e1916872021-12-02T15:03:07ZUrine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children10.1038/s41598-021-91545-02045-2322https://doaj.org/article/5819bbdaba1642aca78d88c48e1916872021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91545-0https://doaj.org/toc/2045-2322Abstract Tuberculosis (TB) is a major cause of morbidity and mortality in children, and early diagnosis and treatment are crucial to reduce long-term morbidity and mortality. In this study, we explore whether urine nuclear magnetic resonance (NMR)-based metabolomics could be used to identify differences in the metabolic response of children with different diagnostic certainty of TB. We included 62 children with signs and symptoms of TB and 55 apparently healthy children. Six of the children with presumptive TB had bacteriologically confirmed TB, 52 children with unconfirmed TB, and 4 children with unlikely TB. Urine metabolic fingerprints were identified using high- and low-field proton NMR platforms and assessed with pattern recognition techniques such as principal components analysis and partial least squares discriminant analysis. We observed differences in the metabolic fingerprint of children with bacteriologically confirmed and unconfirmed TB compared to children with unlikely TB (p = 0.041 and p = 0.013, respectively). Moreover, children with unconfirmed TB with X-rays compatible with TB showed differences in the metabolic fingerprint compared to children with non-pathological X-rays (p = 0.009). Differences in the metabolic fingerprint in children with different diagnostic certainty of TB could contribute to a more accurate characterisation of TB in the paediatric population. The use of metabolomics could be useful to improve the prediction of TB progression and diagnosis in children.Patricia Comella-del-BarrioJosé Luis Izquierdo-GarciaJacqueline GautierMariette Jean Coute DorescaRamón Campos-OlivasClara M. SantiveriBeatriz Muriel-MorenoCristina Prat-AymerichRosa AbellanaTomas M. Pérez-PorcunaLuis E. CuevasJesús Ruiz-CabelloJosé DomínguezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patricia Comella-del-Barrio José Luis Izquierdo-Garcia Jacqueline Gautier Mariette Jean Coute Doresca Ramón Campos-Olivas Clara M. Santiveri Beatriz Muriel-Moreno Cristina Prat-Aymerich Rosa Abellana Tomas M. Pérez-Porcuna Luis E. Cuevas Jesús Ruiz-Cabello José Domínguez Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children
description	Abstract Tuberculosis (TB) is a major cause of morbidity and mortality in children, and early diagnosis and treatment are crucial to reduce long-term morbidity and mortality. In this study, we explore whether urine nuclear magnetic resonance (NMR)-based metabolomics could be used to identify differences in the metabolic response of children with different diagnostic certainty of TB. We included 62 children with signs and symptoms of TB and 55 apparently healthy children. Six of the children with presumptive TB had bacteriologically confirmed TB, 52 children with unconfirmed TB, and 4 children with unlikely TB. Urine metabolic fingerprints were identified using high- and low-field proton NMR platforms and assessed with pattern recognition techniques such as principal components analysis and partial least squares discriminant analysis. We observed differences in the metabolic fingerprint of children with bacteriologically confirmed and unconfirmed TB compared to children with unlikely TB (p = 0.041 and p = 0.013, respectively). Moreover, children with unconfirmed TB with X-rays compatible with TB showed differences in the metabolic fingerprint compared to children with non-pathological X-rays (p = 0.009). Differences in the metabolic fingerprint in children with different diagnostic certainty of TB could contribute to a more accurate characterisation of TB in the paediatric population. The use of metabolomics could be useful to improve the prediction of TB progression and diagnosis in children.
format	article
author	Patricia Comella-del-Barrio José Luis Izquierdo-Garcia Jacqueline Gautier Mariette Jean Coute Doresca Ramón Campos-Olivas Clara M. Santiveri Beatriz Muriel-Moreno Cristina Prat-Aymerich Rosa Abellana Tomas M. Pérez-Porcuna Luis E. Cuevas Jesús Ruiz-Cabello José Domínguez
author_facet	Patricia Comella-del-Barrio José Luis Izquierdo-Garcia Jacqueline Gautier Mariette Jean Coute Doresca Ramón Campos-Olivas Clara M. Santiveri Beatriz Muriel-Moreno Cristina Prat-Aymerich Rosa Abellana Tomas M. Pérez-Porcuna Luis E. Cuevas Jesús Ruiz-Cabello José Domínguez
author_sort	Patricia Comella-del-Barrio
title	Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children
title_short	Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children
title_full	Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children
title_fullStr	Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children
title_full_unstemmed	Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children
title_sort	urine nmr-based tb metabolic fingerprinting for the diagnosis of tb in children
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5819bbdaba1642aca78d88c48e191687
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Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children

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