Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein

Abstract Increased level of CD44 protein in serum is observed in several cancers and is associated with tumor burden and metastasis. Current clinically used detection methods of this protein are time-consuming and use labeled reagents for analysis. Therefore exploring new label-free and fast methods...

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Autores principales:	Aliya Bekmurzayeva, Zhannat Ashikbayeva, Zhuldyz Myrkhiyeva, Aigerim Nugmanova, Madina Shaimerdenova, Takhmina Ayupova, Daniele Tosi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/581b5420632841a08040ff91529ebeb7
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spelling	oai:doaj.org-article:581b5420632841a08040ff91529ebeb72021-12-02T18:51:28ZLabel-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein10.1038/s41598-021-99099-x2045-2322https://doaj.org/article/581b5420632841a08040ff91529ebeb72021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99099-xhttps://doaj.org/toc/2045-2322Abstract Increased level of CD44 protein in serum is observed in several cancers and is associated with tumor burden and metastasis. Current clinically used detection methods of this protein are time-consuming and use labeled reagents for analysis. Therefore exploring new label-free and fast methods for its quantification including its detection in situ is of importance. This study reports the first optical fiber biosensor for CD44 protein detection, based on a spherical fiber optic tip device. The sensor is easily fabricated from an inexpensive material (single-mode fiber widely used in telecommunication) in a fast and robust manner through a CO2 laser splicer. The fabricated sensor responded to refractive index change with a sensitivity of 95.76 dB/RIU. The spherical tip was further functionalized with anti-CD44 antibodies to develop a biosensor and each step of functionalization was verified by an atomic force microscope. The biosensor detected a target of interest with an achieved limit of detection of 17 pM with only minor signal change to two control proteins. Most importantly, concentrations tested in this work are very broad and are within the clinically relevant concentration range. Moreover, the configuration of the proposed biosensor allows its potential incorporation into an in situ system for quantitative detection of this biomarker in a clinical setting.Aliya BekmurzayevaZhannat AshikbayevaZhuldyz MyrkhiyevaAigerim NugmanovaMadina ShaimerdenovaTakhmina AyupovaDaniele TosiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aliya Bekmurzayeva Zhannat Ashikbayeva Zhuldyz Myrkhiyeva Aigerim Nugmanova Madina Shaimerdenova Takhmina Ayupova Daniele Tosi Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein
description	Abstract Increased level of CD44 protein in serum is observed in several cancers and is associated with tumor burden and metastasis. Current clinically used detection methods of this protein are time-consuming and use labeled reagents for analysis. Therefore exploring new label-free and fast methods for its quantification including its detection in situ is of importance. This study reports the first optical fiber biosensor for CD44 protein detection, based on a spherical fiber optic tip device. The sensor is easily fabricated from an inexpensive material (single-mode fiber widely used in telecommunication) in a fast and robust manner through a CO2 laser splicer. The fabricated sensor responded to refractive index change with a sensitivity of 95.76 dB/RIU. The spherical tip was further functionalized with anti-CD44 antibodies to develop a biosensor and each step of functionalization was verified by an atomic force microscope. The biosensor detected a target of interest with an achieved limit of detection of 17 pM with only minor signal change to two control proteins. Most importantly, concentrations tested in this work are very broad and are within the clinically relevant concentration range. Moreover, the configuration of the proposed biosensor allows its potential incorporation into an in situ system for quantitative detection of this biomarker in a clinical setting.
format	article
author	Aliya Bekmurzayeva Zhannat Ashikbayeva Zhuldyz Myrkhiyeva Aigerim Nugmanova Madina Shaimerdenova Takhmina Ayupova Daniele Tosi
author_facet	Aliya Bekmurzayeva Zhannat Ashikbayeva Zhuldyz Myrkhiyeva Aigerim Nugmanova Madina Shaimerdenova Takhmina Ayupova Daniele Tosi
author_sort	Aliya Bekmurzayeva
title	Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein
title_short	Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein
title_full	Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein
title_fullStr	Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein
title_full_unstemmed	Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein
title_sort	label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of cd44 protein
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/581b5420632841a08040ff91529ebeb7
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Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein

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