First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia

Abstract The isolation and molecular typing of Toxoplasma gondii strains provide an essential basis for a better understanding of the parasite’s genetic diversity, determinants of its geographical distribution and associated risks to human health. In this study, we isolated and genetically character...

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Autores principales:	Arwa Lachkhem, Lokman Galal, Ibtissem Lahmar, Karine Passebosc, Homayoun Riahi, Nicolas Plault, Marie-Laure Dardé, Aurélien Mercier, Hamouda Babba
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/58232386f06545c991f4a28cb6beaf15
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spelling	oai:doaj.org-article:58232386f06545c991f4a28cb6beaf152021-12-02T17:39:19ZFirst isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia10.1038/s41598-021-88751-12045-2322https://doaj.org/article/58232386f06545c991f4a28cb6beaf152021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88751-1https://doaj.org/toc/2045-2322Abstract The isolation and molecular typing of Toxoplasma gondii strains provide an essential basis for a better understanding of the parasite’s genetic diversity, determinants of its geographical distribution and associated risks to human health. In this study, we isolated and genetically characterized T. gondii strains from domestic animals in Southern and coastal area of Tunisia. Blood, hearts and/or brains were collected from 766 domestic animals (630 sheep and 136 free-range chickens). Strain isolation from these samples was performed using mouse bioassay and genotyping was carried out with a multiplex PCR technique using 15 microsatellite markers. Thirty viable strains of T. gondii were successfully isolated from tissues of sheep (19/142) and chickens (11/33). In addition, 3 strains could be successfully genotyped from animal tissues for which mouse bioassay was unsuccessful. A large predominance of type II strains (n = 29) was found in the sampled regions, followed by type III (n = 3) and, for the first time in Tunisia, a single isolate of Africa 4 lineage from a sheep. Analyses of population genetics showed the presence of a divergent population of type II lineage in Tunisia, supporting limited recent migrations of strains between Tunisia and other countries of the world.Arwa LachkhemLokman GalalIbtissem LahmarKarine PasseboscHomayoun RiahiNicolas PlaultMarie-Laure DardéAurélien MercierHamouda BabbaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arwa Lachkhem Lokman Galal Ibtissem Lahmar Karine Passebosc Homayoun Riahi Nicolas Plault Marie-Laure Dardé Aurélien Mercier Hamouda Babba First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia
description	Abstract The isolation and molecular typing of Toxoplasma gondii strains provide an essential basis for a better understanding of the parasite’s genetic diversity, determinants of its geographical distribution and associated risks to human health. In this study, we isolated and genetically characterized T. gondii strains from domestic animals in Southern and coastal area of Tunisia. Blood, hearts and/or brains were collected from 766 domestic animals (630 sheep and 136 free-range chickens). Strain isolation from these samples was performed using mouse bioassay and genotyping was carried out with a multiplex PCR technique using 15 microsatellite markers. Thirty viable strains of T. gondii were successfully isolated from tissues of sheep (19/142) and chickens (11/33). In addition, 3 strains could be successfully genotyped from animal tissues for which mouse bioassay was unsuccessful. A large predominance of type II strains (n = 29) was found in the sampled regions, followed by type III (n = 3) and, for the first time in Tunisia, a single isolate of Africa 4 lineage from a sheep. Analyses of population genetics showed the presence of a divergent population of type II lineage in Tunisia, supporting limited recent migrations of strains between Tunisia and other countries of the world.
format	article
author	Arwa Lachkhem Lokman Galal Ibtissem Lahmar Karine Passebosc Homayoun Riahi Nicolas Plault Marie-Laure Dardé Aurélien Mercier Hamouda Babba
author_facet	Arwa Lachkhem Lokman Galal Ibtissem Lahmar Karine Passebosc Homayoun Riahi Nicolas Plault Marie-Laure Dardé Aurélien Mercier Hamouda Babba
author_sort	Arwa Lachkhem
title	First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia
title_short	First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia
title_full	First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia
title_fullStr	First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia
title_full_unstemmed	First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia
title_sort	first isolation and genotyping of toxoplasma gondii strains from domestic animals in tunisia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/58232386f06545c991f4a28cb6beaf15
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First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia

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